Therapeutic study for epidermolysis bullosa using milk with human collagen VII
| Project/Area Number |
22791047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hokkaido University |
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Principal Investigator |
ITO Kei 北海道大学, 大学院・医学研究科, 非常勤講師 (20421977)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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|---|
| Keywords | 皮膚病理学 / 蛋白補充療法 |
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| Research Abstract |
Recessive dystrophic epidermolysis bullosa(RDEB) is a congenital blistering skin disorder of which collagen VII. In this study, we aimed at transgenic mice we created in which human collagen VII is secreted into their milk. We assessed the valuable of the milk with human collagen VII as a novel therapeutic approach for RDEB. First, direct breast feed of neonatal collagen VII knockout mice by the transgenic mice was not successful due to severe blister formation of the EB model mice. Second, local cutaneous injection of purified human collagen VII did not induce adverse immune reactions, suggesting safety of the molecule. However, it was very difficult to purify enough amount of the human collagen VII protein from the milk for in vivo therapeutic study. Recombinant collagen VII may be useful as a novel therapeutic approach for RDEB patients if improved protocol for purification of the molecule will be established.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Prevalent LIPH founder mutations lead to loss of P2Y5 activation ability of PA-PLA1alpha in autosomal recessive hypotrichosis2010
Author(s)
Shinkuma S, Akiyama M, Inoue A, Aoki J, Natsuga K, Nomura T, Arita K, Abe R, Ito K, Nakamura H, Ujiie H, Shibaki A, Suga H, Tsunemi Y, Nishie W, Shimizu H
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Journal Title
Hum Mutat
Volume: 31
Pages: 602-610
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Peer Reviewed
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[Presentation] インフリキシマブ投与乾癬患者25例およびバイオロジクススイッチ5症例を含むウステキヌマブ投与患者20例の使用経験2011
Author(s)
伊藤圭,飯谷麻里,渡邊英里香,大宮若菜,豊永愛恋,小玉和郎,小林仁,安田秀美,浜坂幸吉,村本文男,大田光仁,西村真智子
Organizer
第388回日本皮膚科学会北海道地方会
Place of Presentation
札幌医科大学,札幌
Year and Date
2011-12-17
Related Report
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[Presentation] Type VII collagen deficiency causes defective tooth enamel formation due to poorly differentiated ameloblasts2011
Author(s)
Umemoto H, Akiyama M, Domon T, Nomura T, Nishie W, Shinkuma S, Ito K, Sawamura D, Uitto J, Kitagawa Y, Shimizu H
Organizer
The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
Place of Presentation
Kyoto International Conference Center, Kyoto
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[Presentation] インフリキシマブ投与乾癬患者22例および切り替え5症例を含むウステキヌマブ投与患者10例の使用経験2011
Author(s)
伊藤圭,飯谷麻里,渡邊英里香,大宮若菜,豊永愛恋,小玉和郎,小林仁,安田秀美,浜坂幸吉,村本文男,西村真智子
Organizer
第26回日本乾癬学会学術大会
Place of Presentation
大阪国際会議場,大阪
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[Presentation] 高頻度に認められるLIPH founder変異を有した常染色体劣性乏毛症の4家系2010
Author(s)
新熊悟,秋山真志,夏賀健,乃村俊史,有田賢,阿部理一郎,伊藤圭,中村秀樹,氏家英之,芝木晃彦,西江渉,清水宏
Organizer
第109回日本皮膚科学会総会
Place of Presentation
大阪国際会議場,大阪
Related Report
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