| Project/Area Number |
22791057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUGAYA Makoto 東京大学, 医学部附属病院, 講師 (90334408)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 免疫 / リンパ / 創傷治癒 / 腫瘍免疫 |
|---|
| Research Abstract |
Wound healing was significantly delayed in the transgenic(TG) mice with severe lymphatic dysfunction when compared to wild-type(WT) mice. In wounded skin of TG mice, mast cell numbers were increased, whereas macrophage numbers were decreased. Moreover, increased IL-10 mRNA levels and decreased levels of basic fibroblast growth factor were detected in wounded skin of TG mice compared to WT mice. Similar results were obtained using a different lymphedema model, in which circumferential full thickness skin excision was performed on the tails of WT mice to remove the superficial lymphatics. With regards to tumor immunity, TG mice that had been subcutaneously injected with B16 melanoma cells did not show metastasis in the draining lymph node(LN). Expression levels of TNF-. and IFN-. were significantly lower in the draining LN of TG mice compared to WT mice. By contrast, larger tumor formation was observed in the injected site in TG mice, suggesting that sufficient immune response against tumor cells was not established in the draining LN of TG mice.
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