The development of tailor-made molecular target therapy by circulating tumor cells and microRNAs in melanoma patients

• Project/Area Number: 22791065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Shinshu University
• Principal Investigator: GOTO Yasufumi 信州大学, 医学部, 非常勤講師 (60467181)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 皮膚腫瘍学 / 悪性黒色腫 / microRNA / マイクロビーズ / バイオマーカー / BRAF / KIT / mir214 / 抗癌剤耐性

Research Abstract

We established the methods of isolating circulating tumor cells from melanoma patients by using the magnetic beads and melanoma specific antibodies. CTC genotyping may be useful as a biomarker of molecular-targeted therapy by using BRAF inhibitor for melanoma patients.

Research Products

• [Journal Article] Assessment of BRAF and KIT mutations in Japanese melanoma patients (2012)
  • Author(s): Ashida A, Uhara H, Kiniwa Y, Oguchi M, Murata H, Goto Y, Uchiyama A, Ogawa E, Hayashi K, Koga H, Okuyama R
  • Journal Title: J Dermatol Sci Volume: 5 Pages: 240-242
  • Peer Reviewed
• [Journal Article] Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level (2012)
  • Author(s): Sakaizawa K, Goto Y, Kiniwa Y, et al
  • Journal Title: Br J Cancer Volume: 106 Issue: 5 Pages: 939-946
  • DOI: 10.1038/bjc.2012.12
  • Peer Reviewed
• [Journal Article] Rapid progression of hidradenitis suppurativa in the lower leg of a patient with psoriasis vulgaris (2012)
  • Author(s): Tanaka A, Goto Y, Iwade M, Uhara H, Okuyama R.
  • Journal Title: Acta Derm Venereol Volume: 92(1) Pages: 105-6
  • NAID: 120007100104
  • Peer Reviewed
• [Journal Article] Rapid progression of hidradenitis suppurativa in the lower leg of a patient with psoriasis vulgaris (2012)
  • Author(s): Tanaka A, Goto Y, Iwade M, Uhara H, Okuyama R
  • Journal Title: Acta Derm Venereol Volume: 92 Issue: 1 Pages: 105-6
  • DOI: 10.2340/00015555-1182
  • NAID: 120007100104
  • Peer Reviewed
• [Journal Article] Mutation analysis of BRAF and KIT in circulating melanoma cells at the single cell level. Assessment of BRAF and KIT mutations in Japanese melanoma patients (2012)
  • Author(s): Sakaizawa K, Goto Y, Kiniwa Y, Uchiyama A, Harada K, Shimada S, Saida T, Ferrone S, Takata M, Uhara H, Okuyama R
  • Journal Title: Br J Cancer Volume: 106 Pages: 939-46
  • Peer Reviewed
• [Journal Article] Polyclonality of BRAF mutations in primary melanoma and the selection of mutant alleles during progression (2011)
  • Author(s): Lin J, Goto Y, Murata H, Sakaizawa K, Uchiyama A, Saida T, Takata M
  • Journal Title: Br J Cancer Volume: 11 Pages: 464-468
  • Peer Reviewed
• [Journal Article] THAP5 is a DNA-binding transcriptional repressor that is regulated in melanoma cells during DNA damage-induced cell death (2011)
  • Author(s): Balakrishnan MP, Cilenti L, Ambivero C, Goto Y, Takata M, Turkson J, Li XS, Zervos AS
  • Journal Title: Biochem Biophys Res Commun Volume: 404(1) Pages: 195-200
  • Peer Reviewed
• [Journal Article] Leg Ulcers Associated with Positive Lupus Anticoagulant in Two Cases of Klinefelter's Syndrome (2011)
  • Author(s): Goto Y, Uhara H, Murata H, Koga H, Kosho T, Yamazaki M, Takata M, Okuyama R.
  • Journal Title: Acta Derm Venereol Volume: 91 Pages: 90-91
  • NAID: 120007100108
  • Peer Reviewed
• [Journal Article] THAP5 is a DNA-binding transcriptional repressor that is regulated in melanoma cells during DNA damage-induced cell death (2011)
  • Author(s): Balakrishnan MP, Cilenti L, Ambivero C, Goto Y, Takata M, Turkson J, Li XS, Zervos AS
  • Journal Title: Biochem Biophys Res Commu Volume: 404 Pages: 195-200
  • Peer Reviewed
• [Journal Article] Leg Ulcers Associated with Positive Lupus Anticoagulant in Two Cases of Klinefelter' s Syndrome (2010)
  • Author(s): Goto Y, Uhara H, Murata H, Koga H, Kosho T, Yamazaki M, Takata M, Okuyama R
  • Journal Title: Acta Derm Venereol Pages: 90-91
  • Peer Reviewed
• [Journal Article] Assessment of BRAF and KIT mutations in Japanese melanoma patients
  • Author(s): Ashida A, Uhara H, Kiniwa Y, Oguchi M, Murata H, Goto Y, Uchiyama A, Ogawa E, Hayashi K, Koga H, Okuyama R
  • Journal Title: J Dermatol Sci Volume: (in press)
  • Peer Reviewed
• [Presentation] FABP7 gene expression during malignant melanoma progression : Utility as a prognostic molecular marker (2010)
  • Author(s): 後藤康文
  • Organizer: 第35回日本研究皮膚科学会総会
  • Place of Presentation: 和歌山
  • Year and Date: 2010-12-03
• [Presentation] FABP7 gene expression in association with melanoma progression : Utility as a prognostic marker (2010)
  • Author(s): 後藤康文
  • Organizer: 第35回日本研究皮膚科学会総会
  • Place of Presentation: 和歌山市
  • Year and Date: 2010-12-03
• [Presentation] Activation of Toll-Like Receptor 4 on human melanoma cells induced cell proliferation and migration (2010)
  • Author(s): 後藤康文
  • Organizer: Melanoma Congres2010
  • Place of Presentation: シドニー
  • Year and Date: 2010-11-04
• [Presentation] The activation of Toll-Like Receptor 4 on human melanoma cells induced cell proliferation and migration (2010)
  • Author(s): 後藤康文
  • Organizer: Melanoma Congres2010
  • Place of Presentation: シドニー
  • Year and Date: 2010-11-04
• [Presentation] 血液循環メラノーマ細胞におけるBRAFとKIT遺伝子の解析 (2010)
  • Author(s): 後藤康文
  • Organizer: メラノーマ班会議
  • Place of Presentation: 東京
  • Year and Date: 2010-07-31
• [Presentation] 爪甲メラノーマへの対応 (2010)
  • Author(s): 後藤康文
  • Organizer: 109回日本皮膚科学会総会・教育講演
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-16
• [Presentation] 抗リン脂質抗体症候群(APS)を合併したKlinefelter症候群に生じた下腿潰瘍の2例 (2010)
  • Author(s): 後藤康文
  • Organizer: 109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-16
• [Presentation] 爪甲メラノーマへの対応 (2010)
  • Author(s): 後藤康文
  • Organizer: 109回日本皮膚科学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-04-16
• [Presentation] 抗リン脂質抗体症候群(APS)を合併したKlinefelter症候群に生じた下腿潰瘍の2例 (2010)
  • Author(s): 後藤康文
  • Organizer: 109回日本皮膚科学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-04-16
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-hifu/index.html
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-hifu/index.html