Structural determinant of Humanβ-defensin-3 to stimulate cytokine/chemokine secretion

• Project/Area Number: 22791075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Okayama University
• Principal Investigator: SHIRAFUJI Yoshinori 岡山大学, 岡山大学病院, 助教 (90423285)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ヒトβデフェンシン-3 / αヘリカル / アルギニン / IL-6 / IL-8 / デフェンシン / αヘリクス / サイトカイン

Research Abstract

Humanβ-defensin-3(HBD-3) possesses an ability to stimulate proinflammatory cytokine/chemokine secretion, which links innate and adaptive immunity, as well as antimicrobial activity. Although we hypothesized thatα-helics in N-terminal is a structural determinant for this function and examined the effect on normal human epidermal keratinocytes to secrete IL-6 and-8, the secretion of IL-6 and-8 was associated with two Arg residues in C-terminal unique motif of HBD-3 not withα-helics.

Research Products

• [Journal Article] Elevated expression of paneth cell CRS4C in ileitis-prone samp1/YitFc mice : Regional distribution, subcellular localization, and mechanism of action (2010)
  • Author(s): Shanahan MT, Vidrich A, Shirafuji Y, Dubois CL, Henschen-Edman A, Hagen SJ, Cohn SM, Ouellette AJ
  • Journal Title: J Biol Chem Volume: vol 285 Pages: 7493-504
  • Peer Reviewed
• [Journal Article] Elevated expression of paneth cell CRS4C in ileitis-prone sampl/YitFc mice : Regional distribution, subcellular localization, and mechanism of action. (2010)
  • Author(s): Shanahan MT, Vidrich A, Shirafuji Y, Dubois CL, Henschen-Edman A, Hagen SJ, Cohn SM, Ouellette AJ
  • Journal Title: J Biol Chem Volume: 285 Pages: 7493-7504
  • Peer Reviewed
• [Presentation] Two Arg in C-terminal of human beta-defensin-3 are essential for the stimulation of proinflammatory cytokine secretion in keratinocytes (2011)
  • Author(s): Shirafuji Y, Yasui Y, Morizane S, Iwatsuki K
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 京都
  • Year and Date: 2011-12-10
• [Presentation] Two Arg in C-terminal of human beta-defensin-3 are essential for the stimulation of proinflammatory cytokine secretion in keratinocytes (2011)
  • Author(s): Yoshinori Shirafuji
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-12-10
• [Presentation] Two Arginine residues of C-terminal are essential for the unique salt-insensitive and broad antimicrobial activities of human beta-defensin-3 (2010)
  • Author(s): Yoko Yasui, Yoshinori Shirafuji, Keiji Iwatsuki
  • Organizer: The 35th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 和歌山
  • Year and Date: 2010-12-05
• [Presentation] Two Arginine residues of C-terminal are essential for the unique salt-insensitive and broad antimicrobial activities of human beta-defensin-3 (2010)
  • Author(s): Yoko Yasui, Yoshinori Shirafuji, Keiji Iwatsuki
  • Organizer: 日本研究皮膚科学会 第35回年次学術大会・総会
  • Place of Presentation: 和歌山