| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Epithelial stem cells(EpSCs) and dermal papilla cells(DPCs) are essential for hair follicle morphogenesis. However, in vitro culture methods of those have not been established. In this study, I aimed to develop the isolation of mouse EpSCs and DPCs and their cultivation using Wnt proteins.
Mouse EpSCs were collected by sorting the cells positive for CD34 and CD49f. They could proliferate and maintain the expression of stem cell markers in the passaged cultures in the presence of Wnt-3a. The addition of Wnt-10b to the DPCs promoted their proliferation and maintained their hair follicle induction ability. These effects were observed even in DPCs after 10 passages in the presence of Wnt-10b. Furthermore, the hair-like structures were found in co-cultivation with EpSCs and DPCs in vitro.
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