Analysis of regulatory mechanisms of Notch signaling-induced mast cell maturation and antigen-presenting cell functions

• Project/Area Number: 22791091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Juntendo University
• Principal Investigator: NAKANO Nobuhiro 順天堂大学, 医学研究科, 助教 (30420839)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マスト細胞 / Notchシグナル / MHC class II / mast cell protease / サイトカイン

Research Abstract

Mast cells play an important role in the pathogenesis of allergic inflammatory disorders, such as atopic dermatitis and food allergy. We have previously shown that Notch receptor-mediated signaling contributes to expression of mast cell functions and mast cell maturation. In this study, we elucidated the molecular mechanisms by which Notch signaling induces the antigen-presenting cell functions and the cell maturation in mouse bone marrow-derived mast cells.

Research Products

• [Journal Article] Role of PU. 1 in MHClassI expressiothrough transcriptional regulation of class II transactivator pI in dendritic cells (2012)
  • Author(s): Kitamura N, Yokoyama H, Yashiro T, Nakano N, Nishiyama M, Kanada S, Fukai T, Hara M, Ikeda S, Ogawa H, Okumura K, Nishiyama C
  • Journal Title: J Allergy Clin Immunol Volume: 129 Pages: 814-824
  • Peer Reviewed
• [Journal Article] Notch1-mediated signaling induces MHC class II expression through activation of class II transactivator promoter III in mast cells (2011)
  • Author(s): Nakano N, Nishiyama C, Yagita H, Koyanagi A, Ogawa H, Okumura K
  • Journal Title: J Biol Chem Volume: 286 Pages: 12042-8
  • Peer Reviewed
• [Journal Article] Critical role of transcription factor PU. 1 in the expression of CD80 and CD86 on dendritic cells (2011)
  • Author(s): Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, Kitamura N, Ogawa H, Okumura K
  • Journal Title: Blood Volume: 117 Pages: 2211-22
  • Peer Reviewed
• [Journal Article] マスト細胞機能とNotch (2011)
  • Author(s): 中野信浩
  • Journal Title: 床免疫・アレルギー科 Volume: 55(6) Pages: 589-94
• [Journal Article] 抗原提示細胞としての機能をもつマスト細胞と好塩基球 (2011)
  • Author(s): 中野信浩
  • Journal Title: 化学と生物 Volume: 49(4) Pages: 224-6
• [Journal Article] Role of PU.1 in MHC class II expression through transcriptional regulation of class II transactivator pI in dendritic cells (2011)
  • Author(s): Kitamura N., et al
  • Journal Title: J Allergy Clin Immunol Volume: (未定)(in press) Issue: 3 Pages: 814-824
  • DOI: 10.1016/j.jaci.2011.10.019
  • Peer Reviewed
• [Journal Article] マスト細胞機能とNotch (2011)
  • Author(s): 中野信浩
  • Journal Title: 臨床免疫・アレルギー科 Volume: 55 Pages: 589-594
• [Journal Article] Notch1-mediated signaling induces MHC class II expression through the activation of class II transactivator promoter III in mast cells. (2011)
  • Author(s): Nakano N., et al.
  • Journal Title: J Biol Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. (2011)
  • Author(s): Kanada S., et al.
  • Journal Title: Blood Volume: 117 Pages: 2211-2222
  • Peer Reviewed
• [Journal Article] Suppressive effects of transcription factor GATA-1 on cell type-specific gene expression in dendritic cells (2010)
  • Author(s): Shimokawa N, Nishiyama C, Nakano N, Maeda K, Suzuki R, Hara M, Fukai T, Tokura T, Miyajima H, Nakao A, Ogawa H, Okumura K
  • Journal Title: Immunogenetics Volume: 62 Pages: 421-9
  • Peer Reviewed
• [Journal Article] Opposite effects of Trichostatin A on activation of mast cells by different stimulants (2010)
  • Author(s): Wang QH, Nishiyama C, Nakano N, Kanada S, Hara M, Kitamura N, Shimokawa N, Lu CL, Ogawa H, Okumura K
  • Journal Title: FEBS Lett Volume: 584 Pages: 2315-20
  • Peer Reviewed
• [Journal Article] マスト細胞による抗原提示とTh2細胞の誘導 (2010)
  • Author(s): 中野信浩
  • Journal Title: 臨床免疫・アレルギー科 Volume: 53(6) Pages: 620-5
  • NAID: 40017206588
• [Journal Article] Suppressive effects of transcription factor GATA-1 on cell type-specific gene expression in dendritic cells. (2010)
  • Author(s): Shimokawa N., et al.
  • Journal Title: Immunogenetics Volume: 62 Pages: 421-429
  • Peer Reviewed
• [Journal Article] Opposite effects of Trichostatin A on activation of mast cells by different stimulants. (2010)
  • Author(s): Wang Q.H., et al.
  • Journal Title: FEBS Lett Volume: 584 Pages: 2315-2320
  • Peer Reviewed
• [Presentation] マスト細胞のIL-4産生がNotchシグナルによって増強されるメカニズムの解析 (2012)
  • Author(s): 中野信浩
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] Antigen-presenting cell function induced in mast cells by Notch1-mediated signaling (2011)
  • Author(s): 中野信浩
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 京都
  • Year and Date: 2011-12-09
• [Presentation] Antigen-presenting cell function induced in mast cells by Notch1-mediated signaling (2011)
  • Author(s): 中野信浩
  • Organizer: 日本研究皮膚科学会第36回年次学術大会・総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-12-09
• [Presentation] Tescalcin contributes to maturation of mucosal-type mast cells (2011)
  • Author(s): 中野信浩
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] Tescalcin contributes to maturation of mucosal-type mast cells (2011)
  • Author(s): 中野信浩
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-29
• [Presentation] マスト細胞のIL-4産生がNotchシグナルによって増強される機序の解析 (2011)
  • Author(s): 中野信浩
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-12
• [Presentation] マスト細胞のIL-4産生がNotchシグナルによって増強される機序の解析 (2011)
  • Author(s): 中野信浩
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: グランドプリンスホテル新高輪(東京都)
  • Year and Date: 2011-11-12
• [Presentation] Notchシグナルによるマスト細胞のIL-4産生能の増強 (2011)
  • Author(s): 中野信浩
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 東日本大震災のため要旨集のみ発行、口頭発表中止
• [Presentation] マスト細胞の成熟に寄与する遺伝子Tescalcinの解析 (2010)
  • Author(s): 中野信浩
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/atopy_center/index.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/atopy_center/index.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/atopy_center/index.html