Molecular biological investigation about the effect of heavy-ion beam on normal brain

• Project/Area Number: 22791167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: SUZUKI Yoshiyuki 群馬大学, 大学院・医学系研究科, 准教授 (60334116)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 炭素イオン線 / 悪性脳腫瘍 / 遊走能 / 増感剤 / ニューロン / 神経幹細胞 / 放射線治療 / グリア

Research Abstract

1) Carbon-ion beams were about 10-fold more effective than X-rays in immature hippocampal neurons. X-irradiation immediately induced morphological changes in axons and dendrite. These changes seemed to be caused by x-irradiation evoking depolymerization of microfilament.
2) Under severe hypoxic condition for 1 hour, oxygen enhancement ratio (OER) of X-ray was calculated as 2.7. Also, Under severe hypoxic condition for 24 hour, OER of X-ray was calculated as 2.6. OER was decreased to 1.1 by the combination use of X-ray irradiation and mTOR inhibitor. However, OER was not changed by combination use of carbon-ion beam irradiation and mTOR inhibitor.
3) The RBE of C-beams to X-rays in the normal cerebellum of infant rats with rat organotypic slice culture system was assumed to be 1.4.

Research Products

• [Journal Article] Suppression of HIF-1α and Radiation Resistance in the Acute Hypoxic Condition (2012)
  • Author(s): Oike T, Suzuki Y, Al-Jahdari W, Mobaraki A, Saitoh JI, Torikai K, Shirai K, Nakano T.
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Pages: 141-145
  • Peer Reviewed
• [Journal Article] Evaluation of the Relative Biological Effectiveness using Rat Organotypic Slice Culture System on Cerebellum (2012)
  • Author(s): Yoshida Y, Suzuki Y, Al-Jahdari WS, Hamada N, Funayama T, Shirai K, Katoh H, Sakashita T, Kobayashi Y, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 53(1) Pages: 87-92
  • Peer Reviewed
• [Journal Article] Evaluation of the Relative Biological Effectiveness using Rat Organotypic Slice Culture System on Cerebellum (2012)
  • Author(s): Yoshida Y, Suzuki Y, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 53 Pages: 87-92
  • Peer Reviewed
• [Journal Article] Expression of hypoxia-inducible factor 1α predicts clinical outcome after preoperative hyperthermo-chemoradiotherapy for locally advanced rectal cancer (2012)
  • Author(s): Shioya M, Takahashi T, Ishikawa H, Sakurai H, Ebara T, Suzuki Y, et al.
  • Journal Title: J Radiat Res Volume: 53 Pages: 87-92
  • NAID: 10029653265
  • Peer Reviewed
• [Journal Article] Reduction of nitric oxide level enhances the radiosensitivity of hypoxic non-small cell lung cancer (2011)
  • Author(s): Saleem W, Suzuki Y, Mobaraki A, Yoshida Y, Noda S, Saitoh JI, Nakano T.
  • Journal Title: Cancer Sci Volume: 102(12) Pages: 2150-6
  • NAID: 10030245759
  • Peer Reviewed
• [Journal Article] Expression of hypoxia-inducible factor 1α predicts clinical outcome after preoperative hyperthermo-chemoradiotherapy for locally advanced rectal cancer (2011)
  • Author(s): Shioya M, Takahashi T, Ishikawa H, Sakurai H, Ebara T, Suzuki Y, Saitoh J, Ohno T, Asao T, Hiroyuki K, Nakano T
  • Journal Title: J Radiat Res (Tokyo) Volume: 52(6) Pages: 821-7
  • NAID: 10029653265
  • Peer Reviewed
• [Journal Article] High-dose-rate Interstitial Brachytherapy with Computed Tomography-based Treatment Planning for Patients with Locally Advanced Uterine Cervical Carcinoma (2011)
  • Author(s): Saitoh J, Ohno T, Sakurai H, Katoh H, Wakatsuki M, Noda SE, Suzuki Y, Sibuya K, Takahashi T, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 52(4) Pages: 490-5
  • NAID: 10029122290
  • Peer Reviewed
• [Journal Article] Development of head module for multi-head Si/CdTe Compton camera for medical applications (2011)
  • Author(s): Yamaguchi M, Kawachi N, Suzui N, Fujimaki S, Kamiya T, Odaka H, Ishikawa S, Kokubun M, Watanabe S, Takahashi T, Shimada H, Arakawa K, Suzuki Y, Torikai K, Yoshida Y, Nakano T. Nucl
  • Journal Title: Instr. Meth. Phys. Res. Volume: 648
  • Peer Reviewed
• [Journal Article] Pseudo-progression event during temozolomide combining chemoradiotherapy for malignant glioma (2011)
  • Author(s): Suzuki Y, Saleem W, Satoh Y, Wakatsuki M, Shirai K, Sugawara K, Noda SE, Okamoto M, Yoshimoto Y, Nakano T
  • Journal Title: Recent Advances and Research Updates Volume: 12 Pages: 315-9
• [Journal Article] Radiation-induced ICAM-1 expression via TGF-β1 pathway on human umbilical vein endothelial cells ; comparison between X-ray and carbon-ion beam irradiation (2011)
  • Author(s): Kiyohara H, Ishizaki Y, Suzuki Y, Katoh H, Hamada N, Ohno T, Takahashi T, Kobayashi Y, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 52(3) Pages: 287-92
  • NAID: 10028111131
  • Peer Reviewed
• [Journal Article] Intracavitary combined with CT-guided interstitial brachytherapy for locally advanced uterine cervical cancer : introduction of the technique and a casepresentation. (2011)
  • Author(s): Wakatsuki M, Ohno T, Yoshida D, Noda SE, Saitoh J, Shibuya K, Katoh H, Suzuki Y, Takahashi T, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 52(1) Pages: 54-8
  • NAID: 10027418268
  • Peer Reviewed
• [Journal Article] MK615, a prospective anti-proliferative agent, enhances CD4/CD8 ratio after exposure to irradiation (2011)
  • Author(s): Al-Jahdari WS, Sakurai H, Yoshida Y, Mobaraki A, Suzuki Y, Nakano T.
  • Journal Title: Int J Radiat Biol Volume: 87(1) Pages: 81-90
  • Peer Reviewed
• [Journal Article] Reduction of nitric oxide level enhances the radiosensitivity of hypoxic non-small cell lung cancer (2011)
  • Author(s): Saleem W, Suzuki Y, et al.
  • Journal Title: Cancer Sci Volume: 102 Pages: 2150-2156
  • NAID: 10030245759
  • Peer Reviewed
• [Journal Article] Pseudo-progression event during temozolomide combining chemoradiotherapy for malignant glioma (2011)
  • Author(s): Suzuki Y, et al.
  • Journal Title: Recent Advances and Research Updates Volume: 11 Pages: 315-319
  • Peer Reviewed
• [Journal Article] High-dose-rate Interstitial Brachytherapy with Computed Tomography-based Treatment Planning for Patients with Locally Advanced Uterine Cervical Carcinoma (2011)
  • Author(s): Saitoh J, Ohno T, Sakurai H, Katoh H, Wakatsuki M, Noda SE, Suzuki Y, et al.
  • Journal Title: J Radiat Res Volume: 52 Pages: 490-495
  • NAID: 10029122290
  • Peer Reviewed
• [Journal Article] Development of head module for multi-head Si/CdTe Compton camera for medical applications (2011)
  • Author(s): Yamaguchi M, Kawachi N, Suzui N, Fujimaki S, Kamiya T, Odaka H, Ishikawa S, Kokubun M, Watanabe S, Takahashi T, Shimada H, Arakawa K, Suzuki Y, et al.
  • Journal Title: Nucl.Instr.Meth.Phys.Res.A Volume: 648 Pages: 2-7
  • Peer Reviewed
• [Journal Article] Radiation-induced ICAM-1 expression via TGF-β1 pathway on human umbilical vein endothelial cells ; comparison between X-ray and carbon-ion beam irradiation (2011)
  • Author(s): Kiyohara H, Ishizaki Y, Suzuki Y, et al.
  • Journal Title: J Radiat Res Volume: 52 Pages: 287-292
  • NAID: 10028111131
  • Peer Reviewed
• [Journal Article] Intracavitary combined with CT-guided interstitial brachytherapy for locally advanced uterine cervical cancer : introduction of the technique and a case presentation. (2011)
  • Author(s): Wakatsuki M, Ohno T, Yoshida D, Noda SE, Saitoh J, Shibuya K, Katoh H, Suzuki Y, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 52 Pages: 54-58
  • NAID: 10027418268
  • Peer Reviewed
• [Journal Article] MK615, a prospective anti-proliferative agent, enhances CD4/CD8 ratio after exposure to irradiation. (2011)
  • Author(s): Al-Jahdari WS, Sakurai H, Yoshida Y, Mobaraki A, Suzuki Y, Nakano T.
  • Journal Title: Int J Radiat Biol. Volume: 87 Pages: 81-90
  • Peer Reviewed
• [Journal Article] The Benefit of Small Bowel and Pelvic Bone Sparing in Excluding Common Iliac Lymph Node Region from Conventional Radiation Fields in Patients with Uterine Cervical Cancer : A Dosimetric Study (2010)
  • Author(s): Oike T, Ohno T, Wakatsuki M, Noda SE, Saitoh JI, Mizukami T, Yoshimoto Y, Okonogi N, Katoh H, Shibuya K, Suzuki Y, Ishikawa H, Ebara T, Takahashi T, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(6) Pages: 715-21
  • NAID: 10026964188
  • Peer Reviewed
• [Journal Article] Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature hippocampal neurons (2010)
  • Author(s): Kaminuma T, Suzuki Y, Shirai K, Mizui T, Noda SE, Yoshida Y, Funayama T, Takahashi T, Kobayashi Y, Shirao T, Nakano T
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(6) Pages: 627-31
  • NAID: 10026963838
  • Peer Reviewed
• [Journal Article] Influence of Histological Subtype on Survival after Combined Therapy of Surgery and Radiation in WHO Grade 3 Glioma (2010)
  • Author(s): Shirai K, Suzuki Y, Okamoto M, Wakatsuki M, Noda SE, Takahashi T, Ishiuchi S, Hasegawa M, Nakazato Y, Nakano T
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(5) Pages: 589-94
  • NAID: 10026644482
  • Peer Reviewed
• [Journal Article] Protective Effect of Urinary Trypsin Inhibitor on the Development of Radiation-Induced Lung Fibrosis in Mice (2010)
  • Author(s): Katoh H, Ishikawa H, Hasegawa M, Yoshida Y, Suzuki Y, Ohno T, Takahashi T, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(3) Pages: 325-32
  • NAID: 10026471232
  • Peer Reviewed
• [Journal Article] Higher pAkt expression predicts a significant worse prognosis in glioblastomas (2010)
  • Author(s): Suzuki Y, Shirai K, Oka K, Mobaraki A, Yoshida Y, Noda SE, Okamoto M, Suzuki Y, Itoh J, Itoh H, Ishiuchi S, Nakano T.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(3) Pages: 343-8
  • NAID: 10026471315
  • Peer Reviewed
• [Journal Article] Current advancement in radiation therapy for uterine cervical cancer (2010)
  • Author(s): Nakano T, Ohno T, Ishikawa H, Suzuki Y, Takahashi T
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 1-8
  • NAID: 10025913850
  • Peer Reviewed
• [Journal Article] Survivin is a Potential Mediator of Prostate Cancer Metastasis (2010)
  • Author(s): Zhang M, Coen JJ, Suzuki Y, Siedow MR, Khor LY, Pollack A, Zietman AL, Shipley WU, Chakravarti A
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 78(4) Pages: 1095-103
  • Peer Reviewed
• [Journal Article] Higher pAkt expression predicts a significant worse prognosis in glioblastomas. (2010)
  • Author(s): Suzuki Y, Shirai K, Oka K, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 343-348
  • NAID: 10026471315
  • Peer Reviewed
• [Journal Article] Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature hippocampal neurons (2010)
  • Author(s): Kaminuma T, Suzuki Y, Shirai K, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 627-631
  • NAID: 10026963838
  • Peer Reviewed
• [Journal Article] Influence of Histological Subtype on Survival after Combined Therapy of Surgery and Radiation in WHO Grade 3 Glioma (2010)
  • Author(s): Shirai K, Suzuki Y, Okamoto M, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 589-594
  • NAID: 10026644482
  • Peer Reviewed
• [Journal Article] Radiation-induced ICAM-1 expression via TGF-β1 pathway on human umbilical vein endothelial cells ; comparison between X-ray and carbon-ion beam irradiation (2010)
  • Author(s): Kiyohara H, Ishizaki Y, Suzuki Y, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51(in press)
  • NAID: 10028111131
  • Peer Reviewed
• [Journal Article] The Benefit of Small Bowel and Pelvic Bone Sparing in Excluding Common Iliac Lymph Node Region from Conventional Radiation Fields in Patients with Uterine Cervical Cancer : A Dosimetric Study (2010)
  • Author(s): Oike T, Ohno T, Wakatsuki M, Noda SE, Saitoh JI, Mizukami T, Yoshimoto Y, Okonogi N, Katoh H, Shibuya K, Suzuki Y, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 715-721
  • NAID: 10026964188
  • Peer Reviewed
• [Journal Article] Protective Effect of Urinary Trypsin Inhibitor on the Development of Radiation-Induced Lung Fibrosis in Mice. (2010)
  • Author(s): Katoh H, Ishikawa H, Hasegawa M, Yoshida Y, Suzuki Y, et al.
  • Journal Title: J Radiat Res (Tokyo) Volume: 51 Pages: 325-332
  • NAID: 10026471232
  • Peer Reviewed
• [Journal Article] Survivin is a Potential Mediator of Prostate Cancer Metastasis (2010)
  • Author(s): Zhang M, Coen JJ, Suzuki Y, et al.
  • Journal Title: Int J Radiat Oncol Biol Phys. Volume: 78 Pages: 1095-1103
  • Peer Reviewed
• [Journal Article] 脳神経細胞の放射線感受性 (2010)
  • Author(s): 鈴木義行、吉田由香里, 他
  • Journal Title: 放射線生物研究 Volume: 45(招待) Pages: 236-248
  • NAID: 40017338094
• [Presentation] 放射線による抗腫瘍免疫の活性化～免疫放射線療法の可能性～ (2011)
  • Author(s): 鈴木義行
  • Organizer: 日本放射線腫瘍学会第24回大会
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)(招待講演)
  • Year and Date: 2011-11-18
• [Presentation] 高精度放射線治療への応用を目指した次世代RI診断機器Si/CdTeコンプトンカメラの開発 (2011)
  • Author(s): 鈴木義行, 他
  • Organizer: 日本放射線腫瘍学会第24回大会
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)
  • Year and Date: 2011-11-17
• [Presentation] Radiation induced neural sell death (apoptosis) (2011)
  • Author(s): Yoshiyuki Suzuki
  • Organizer: The first meeting of International Society for Radiation Neurobiology
  • Place of Presentation: Gunma
  • Year and Date: 2011-01-29
• [Presentation] Radiation induced neural sell death (apoptosis) (2011)
  • Author(s): Yoshiyuki Suzuki
  • Organizer: The first meeting of International Society for Radiation Neurobiology
  • Place of Presentation: To-jyo hall, Gunma Univ.
  • Year and Date: 2011-01-29
• [Presentation] 重粒子線治療 ～群馬大学重粒子線医学センターの現状、及び、中枢神経系腫瘍に対する (2011)
  • Author(s): 鈴木義行
  • Organizer: 第46回群馬脳腫瘍研究会
  • Place of Presentation: 前橋
  • Year and Date: 2011-01-15
• [Presentation] 重粒子線治療～群馬大学重粒子線医学センターの現状、及び、中枢神経系腫瘍に対する今後の展望～ (2011)
  • Author(s): 鈴木義行
  • Organizer: 第46回群馬脳腫瘍研究会
  • Place of Presentation: 前橋商工会議所
  • Year and Date: 2011-01-15
• [Presentation] 放射線による抗腫瘍免疫の活性化 (2011)
  • Author(s): 鈴木義行
  • Organizer: 日本放射線腫瘍学会第24回大会
  • Place of Presentation: 神戸
• [Presentation] 高精度放射線治療への応用を目指した次世代RI診断機器Si/CdTeコンプトンカメラの開発 (2011)
  • Author(s): 鈴木義行, 山口充孝, 小高裕和, 武田伸一郎, 吉田由香里, 神谷富裕, 河地有木, 荒川和夫, 高橋忠幸, 中野隆史
  • Organizer: 日本放射線腫瘍学会第24回大会
  • Place of Presentation: 神戸
• [Presentation] X ray Irradiation Induces Acute Depolymerization of Axonal and Dendritic Microfilaments in Cultured Neuron (2011)
  • Author(s): Suzuki Y, Hino M, Shirai K, Yoshida Y, Mizui T, Hanamura K, Shirao T, Nakano T
  • Organizer: American Society for Radiation Oncology 53th annual meeting
  • Place of Presentation: Miami Beach Convention Center, Miami Beach, FL, USA
• [Presentation] X ray Irradiation Induces Acute Depolymerization of Axonal and Dendritic Microfilaments in Cultured Neuron (2011)
  • Author(s): Suzuki Y, et al.
  • Organizer: The 53th Annual meeting of the American Sciety for Radiation Oncology
  • Place of Presentation: Miami Beach Convention Center, Miami Beach, USA
• [Presentation] Changes of Bone Mineral Densities, Serum Female Hormones and Markers of Bone Resorption after Pelvic Radiation Therapy in Patients With Uterine Cervical Carcinoma (2011)
  • Author(s): Saitoh JI, Suzuki Y, et al.
  • Organizer: The 53th Annual meeting of the American Sciety for Radiation Oncology
  • Place of Presentation: Miami Beach Convention Center, Miami Beach, USA
• [Presentation] The Effect Of HIF-1α On Radioresistance In Acute Hypoxia, In Vitro (2011)
  • Author(s): Oike T, Suzuki Y, et al.
  • Organizer: The 53th Annual meeting of the American Sciety for Radiation Oncology
  • Place of Presentation: Miami Beach Convention Center, Miami Beach, USA
• [Presentation] A role of radiosurgery for functinal preservation : from personal ezperiences of 4500 patients treated wuth microsurgery, Gamma Knife and CyberKnife radiosurgery (2011)
  • Author(s): Inoue KH, Suzuki Y, Takahashi T, Sakurai H
  • Organizer: 10th International Stereotactic Radiosurgery Society Congress
  • Place of Presentation: Paris, France
• [Presentation] 重粒子線治療 (2010)
  • Author(s): 鈴木義行
  • Organizer: 第二回日本放射線外科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 重粒子線治療 (2010)
  • Author(s): 鈴木義行
  • Organizer: 第二回日本放射線外科学会
  • Place of Presentation: 東京 ホテルジュラク
  • Year and Date: 2010-11-27
• [Presentation] Radiation-induced anti-tumor immunity and its role for therapeutic efficacy in a mouse tumor model (2010)
  • Author(s): Yoshimoto Y, Suzuki Y, et al.
  • Organizer: 52th ASTRO annual meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] ニューロンとグリアの放射線感受性 (2010)
  • Author(s): 工藤滋弘, 鈴木義行, 他
  • Organizer: 日本放射線腫瘍学会第23回学術大会
  • Place of Presentation: 東京ベイホテル東急
• [Presentation] 低酸素状態下においてHIF-1α阻害剤が放射線感受性に与える影響の検討 (2010)
  • Author(s): 尾池貴洋、鈴木義行, 他
  • Organizer: 日本放射線腫瘍学会第23回学術大会
  • Place of Presentation: 東京ベイホテル東急
• [Book] Textbook of Radiosurgery:総論5章F (2012)
  • Author(s): 白井克幸, 鈴木義行
  • Publisher: メディカ出版
• [Book] Textbook of Radiosurgery:各論2章(12) (2012)
  • Author(s): 岡本雅彦, 白井克幸, 鈴木義行
  • Publisher: メディカ出版
• [Book] Textbook of Radiosurgery (2012)
  • Author(s): 白井克幸、鈴木義行
  • Publisher: メディカ出版
• [Book] Textbook of Radiosurgery (2012)
  • Author(s): 岡本雅彦、白井克幸、鈴木義行
  • Publisher: メディカ出版
• [Book] 免疫細胞療法II:放射線治療 (2011)
  • Author(s): 鈴木義行, 吉本由哉
  • Publisher: 幻冬舎
• [Book] 免疫細胞療法II (2011)
  • Author(s): 鈴木義行、吉本由哉
  • Publisher: 幻冬舎
• [Book] がん・放射線療法:放射線生物学分子生物学的アプローチ (2010)
  • Author(s): 鈴木義行
  • Publisher: 篠原出版新社
• [Book] がん放射線治療2010 (2010)
  • Author(s): 鈴木義行(分担執筆)
  • Publisher: 篠原出版新社