GRP78 is a novel target for radiation therapy in malignant glioma

• Project/Area Number: 22791170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: NODA Shinei 群馬大学, 医学部, 助教 (60396645)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経膠芽腫 / GRP78 / 放射線治療 / 放射線腫瘍学 / shRNA

Research Abstract

Stable knockdown cell lines of GRP78 using lentiviral based sh-RNA transduction were generated in 3 glioblastoma (GBM) cell lines. GBM cell lines were exposed 6 Gy of gamma radiation and subsequently evaluated to determine the extent of double strand DNA damage using comet and gamma-H2Ax assay and the radiosensitivity using colony formation assay and MTS assay. Apoptosis was evaluated using flowcytometry and westernblot assay.

Research Products

• [Journal Article] Dose Volume Analysis of Radiotherapy for Inoperable Patients with Stage I-II Endometrial Carcinoma (2011)
  • Author(s): Ohkubo Y, Noda SE, Nakano T.
  • Journal Title: J Radiat Res Volume: 52巻 Pages: 666-73
  • NAID: 10029456132
  • Peer Reviewed
• [Journal Article] High-dose-rate interstitial brachytherapy with computed tomography-based treatment planning for patients with locally advanced uterine cervical carcinoma (2011)
  • Author(s): Saitoh J, Noda SE, Nakano T.
  • Journal Title: J Radiat Res Volume: 52巻 Pages: 490-5
  • NAID: 10029122290
  • Peer Reviewed
• [Journal Article] Dose Volume Analysis of Radiotherapy for Inoperable Patients with Stage I-II Endometrial Carcinoma (2011)
  • Author(s): Ohkubo Y, Noda SE,, Nakano T
  • Journal Title: J Radiat Res Volume: 52 Pages: 666-73
  • NAID: 10029456132
  • Peer Reviewed
• [Journal Article] High-dose-rate interstitial brachytherapy with computed tomography-based treatment planning for patients with locally advanced uterine cervical carcinoma (2011)
  • Author(s): Saitoh J, Noda SE, Nakano T
  • Journal Title: J Radiat Res Volume: 52 Pages: 490-5
  • NAID: 10029122290
  • Peer Reviewed
• [Journal Article] Intracavitary combined with CT-guided interstitial brachytherapy for locally advanced uterine cervical cancer : introduction of the technique and a case presentation (2011)
  • Author(s): Wakatsuki M, Noda SE, Nakano T
  • Journal Title: J Radiat Res Volume: 52 Pages: 54-8
  • NAID: 10027418268
  • Peer Reviewed
• [Journal Article] Intracavitary combined with CT-guided interstitial brachytherapy for locally advanced uterine cervical cancer : introduction of the technique and a case presentation. (2011)
  • Author(s): Wakatsuki M
  • Journal Title: J Radiat Res (Tokyo). Volume: 52 Pages: 54-58
  • Peer Reviewed
• [Journal Article] The benefit of small bowel and pelvic bone sparing in excluding common iliaclymph node region from conventional radiation fields in patients with uterine cervical cancer : a dosimetric study. (2010)
  • Author(s): Oike T
  • Journal Title: J Radiat Res (Tokyo). Volume: 51 Pages: 715-721
  • Peer Reviewed
• [Journal Article] Effectiveness of carbon-ion beams for apoptosis induction in rat primaryimmature hippocampal neurons. (2010)
  • Author(s): Kaminuma T
  • Journal Title: J Radiat Res (Tokyo). Volume: 51 Pages: 627-631
  • Peer Reviewed
• [Journal Article] Influence of histological subtype on survival after combined therapy of surgery and radiation in WHO grade 3 glioma. (2010)
  • Author(s): Shirai K
  • Journal Title: J Radiat Res (Tokyo). Volume: 51 Pages: 589-594
  • Peer Reviewed
• [Journal Article] Higher pAkt expression predicts a significant worse prognosis in glioblastomas. (2010)
  • Author(s): Suzuki Y
  • Journal Title: J Radiat Res (Tokyo). Volume: 51 Pages: 343-348
  • Peer Reviewed
• [Journal Article] マイクロセレクトロンによる治療 (2010)
  • Author(s): 野田真永
  • Journal Title: PET journal Volume: 11 Pages: 29-31
• [Presentation] リフレッシャーコース(1)子宮頸癌 (2011)
  • Author(s): 野田真永
  • Organizer: 日本放射線腫瘍学会小線源治療部会第13回研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-14
• [Presentation] 早期子宮頸癌外部照射における総腸骨リンパ節領域省略の可能性 (2010)
  • Author(s): 野田真永
  • Organizer: 日本放射線腫瘍学会第23回学術大会
  • Place of Presentation: 東京ベイ舞浜ホテル
  • Year and Date: 2010-11-20