Fundamental research of X-ray and carbon ion beam radiation sensitivity for the treatment of hypoxic tumor cells and investigation of its molecular biological mechanism.

• Project/Area Number: 22791171
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: SAITOH Jun-ichi 群馬大学, 大学院・医学系研究科, 講師 (70572816)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 放射線感受性 / 低酸素 / HIF-1α / 一酸化窒素

Research Abstract

Changes of the expression of hypoxic marker HIF-1αand radiation sensitivity under hypoxic conditions with HIF-1αinhibitor were investigated. Hypoxic tumor cells were exposed to radiation with or without HIF-1αinhibitor YC-1 or nitric oxide synthase inhibitor L-NMMA. The expressions of HIF-1αunder hypoxic conditions (>0.1mmHg) were inhibited with YC-1 or L-NMMA, but radiosensitizing effects with these drugs under acute hypoxic conditions were not observed.

Research Products

• [Journal Article] Suppression of HIF-1α expression and radiation resistance in acute hypoxic conditions (2012)
  • Author(s): Oike T, Suzuki Y, Wael S, Mobaraki A, Saitoh J, Torikai K, Shirai K, Nakano T.
  • Journal Title: EXPERIMENTAL AND THERAPEUTIC MEDICINE Volume: 3 Pages: 141-145
  • Peer Reviewed
• [Journal Article] Reduction of nitric oxide level enhances the radiosensitivity of hypoxic non-small cell lung cancer (2011)
  • Author(s): Wael S, Suzuki Y, Mobaraki A, Yoshida Y, Noda S, Saitoh J, Nakano T.
  • Journal Title: Cancer Science Volume: 102 Pages: 2150-2156
  • Peer Reviewed
• [Journal Article] Reduction of nitric oxide level enhances the radiosensitivity of hypoxic non-small cell lung cancer (2011)
  • Author(s): Wael Saleem, et al.
  • Journal Title: Cancer Science Volume: Vol.102 Issue: 12 Pages: 2150-2156
  • DOI: 10.1111/j.1349-7006.2011.02095.x
  • Peer Reviewed
• [Presentation] The Effect of HIF-1α on Radioresistance in Acute Hypoxia, In Vitro (2011)
  • Author(s): T. Oike, et al.
  • Organizer: ASTRO 53rd Annual Meeting
  • Place of Presentation: Miami, Florida (USA)
• [Presentation] 急性低酸素モデルにおいてHIF-1α阻害剤が放射線感受性に与える影響の検討 (2010)
  • Author(s): 尾池貴洋, 他
  • Organizer: 日本放射線腫瘍学会第23回学術大会
  • Place of Presentation: 幕張,千葉
  • Year and Date: 2010-11-20
• [Presentation] 急性低酸素モデルにおいてHIF-1α阻害剤が放射線感受性に与える影響の検討 (2010)
  • Author(s): 尾池貴洋, 他
  • Organizer: 日本放射線腫瘍学会第23回学術大会
  • Place of Presentation: 東京ベイ舞浜ホテル クラブリゾート(千葉県)
  • Year and Date: 2010-11-20