Acceleration of Cerebral Blood Flow and Oxygen Metabolism Measurement with Injectable 15O labeled Radiotracers and Effectiveness of Drug Treatment

• Project/Area Number: 22791180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Kanazawa University
• Principal Investigator: KOBAYASHI Masato 金沢大学, 保健学系, 助教 (30444235)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 核医学(PET) / 脳循環代謝 / 放射線 / 画像解析 / 酸素-15 / 人工赤血球 / 放射性同位元素 / 放射線技術 / 画像診断技術 / 放射性酸素-15

Research Abstract

We developed cerebral blood flow and oxygen metabolism measurement combining injectable 15O labeled artificial blood cell and noninvasive rapid steady-state method. The cerebral blood flow and oxygen metabolism of normal and cerebral ischemia-reperfusion model rat was measured and then effectiveness of drug treatment was evaluated using this method. New rapid steady-state method was developed by combining membrane plasma separator to conventional steady-state method and cerebral blood flow and oxygen metabolism of normal and cerebral occlusion region was calculated easily, rapidly and accurately. Influence of isoflurane gas anaesthesia was confirmed using the method.

Research Products

• [Journal Article] Cerebral Oxygen Metabolism of ratsusing injectable 15O-oxygen with asteady-state method (2012)
  • Author(s): Kobayashi M., Mori T., Kiyono Y., Tiwari VN., Maruyama R., Kawai K., Okazawa H.
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism Volume: 32 Pages: 33-40
  • Peer Reviewed
• [Journal Article] Cerebral Oxygen Metabolism of rats using injectable ^<15>O-oxygen with a steady-state method (2012)
  • Author(s): Kobayashi M, Mori T, Kiyono Y, Tiwari VN, Maruyama R, Kawai K, Okazawa H
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism Volume: 32 Pages: 33-40
  • Peer Reviewed
• [Journal Article] Developmentof an H215O steady-state method combining abolus and slow increasing injection witha multi-programming syringe pump (2011)
  • Author(s): Kobayashi M., Kiyono Y., Maruyama R., Mori T., Kawai K., Okazawa H.
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism Volume: 31 Pages: 527-534
  • Peer Reviewed
• [Journal Article] Development of an H_2^<15>O steady-state method combining a bolus and slow increasing injection with a multi-programming syringe pump. (2011)
  • Author(s): Kobayashi M, Kiyono Y, Maruyama R, Mori T, Kawai K, Okazawa H.
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism Volume: 31 Pages: 527-534
  • Peer Reviewed
• [Presentation] 5O酸素ガス標識ヘモグロビンを使用した脳循環代謝測定法の開発 (2011)
  • Author(s): 小林正和
  • Organizer: 第14回福井大学高エネルギー医学研究センター研究発表会
  • Place of Presentation: 福井大学高エネルギー研究センター(福井県)
  • Year and Date: 2011-07-29
• [Presentation] ^<15>O酸素ガス標識ヘモグロビンを使用した脳循環代謝測定法の開発 (2011)
  • Author(s): 小林正和, 森哲也, 清野泰, 丸山力哉, 川井恵一, 岡沢秀彦
  • Organizer: 第14回福井大学高エネルギー医学研究センター研究発表会
  • Place of Presentation: 福井大学高エネルギー医学研究センター(福開県)
  • Year and Date: 2011-07-29
• [Presentation] Development of a newsteady-state method using injectable 15O2for measurement of oxygen metabolism inthe rat brain (2011)
  • Author(s): Kobayashi M.
  • Organizer: The 25rd International Symposium on Cerebral Blood Flow and Metabolism & the 10th International Conference on Quantification of Brain Function with PET
  • Place of Presentation: Centre de ConvencionsInternacional de Barcelona(Spain)
• [Presentation] Development of a new steady-state method using injectable ^<15>O_2 for measurement of oxygen metabolism in the rat brain (2011)
  • Author(s): Kobayashi M, Mori T, Kiyono Y, Tiwari VN, Maruyama R, Kawai K, Okazawa H
  • Organizer: The 25rd International Symposium on Cerebral Blood Flow and Metabolism & the 10th Intemational Conference on Quantification of Brain Function with PET
  • Place of Presentation: Centre de Convencions Intemacional de Barcelona (Spain)
• [Presentation] ^<15>Oガス標識ヘモグロビンを用いた脳循環代謝測定の検討 (2010)
  • Author(s): 小林正和, 森哲也, 清野泰, 岡優治, 大江和代, 高橋典也, 灘村崇裕, 川井恵一, 岡沢秀彦
  • Organizer: 第50回日本核医学会学術総会
  • Place of Presentation: 埼玉県さいたま市(埼玉県)
  • Year and Date: 2010-11-12
• [Presentation] ガス標識ヘモグロビンを用いた脳循環代謝測定の検討 (2010)
  • Author(s): 小林正和
  • Organizer: 第50回日本核医学会学術総会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
• [Patent(Industrial Property Rights)] 分子イメージングにより代謝機能を測定するための検査薬 (2012)
  • Inventor(s): 川井恵一,玉井郁巳,國嶋崇隆,中西猛夫,小林正和
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2012-044231
  • Filing Date: 2012-02-29
• [Patent(Industrial Property Rights)] 分子イメージングにより代謝機能を測定するための検査薬 (2012)
  • Inventor(s): 川井惠一, 玉井郁巳, 國嶋崇隆, 中西猛夫, 小林正和
  • Industrial Property Rights Holder: 国立大学法人金沢大学
  • Industrial Property Number: 2012-044231
  • Filing Date: 2012-02-29