Analysis of molecular targeting radiosensitization using DPYD depletion
| Project/Area Number |
22791207
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 放射線治療生物学 / 放射線増感剤 / 放射線 |
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| Research Abstract |
Gimeracil, an inhibitor of dihydropyrimidine dehydrogenase(DPYD), partially inhibits homologous recombination(HR) repair and has a radiosensitizing effect. We investigated the mechanisms of sensitization of radiation by DPYD inhibition using DLD-1 cells treated with siRNA for DPYD. DPYD depletion by siRNA significantly restrained the formation of radiation-induced foci of Rad51 and RPA, whereas it increased the number of foci of NBS1. The numbers of colocalization of NBS1 and RPA foci in DPYD-depleted cells after radiation were significantly smaller than in the control cells. The phosphorylation of RPA by irradiation was partially suppressed in DPYD-depleted cells, suggesting that DPYD depletion may partially inhibit DNA repair with HR by suppressing phosphorylation of RPA.
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Report
(3 results)
Research Products
(10 results)
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[Presentation] Radiosensitizing effects of Gimeracil2010
Author(s)
Masanori Someya, Masaru Takagi, Koh擁chi Sakata, Hiroshi Tauchi, Yoshihisa Matsumoto, Masato Hareyama, Masakazu Fukushima
Organizer
第56回Radiation Research Society
Place of Presentation
アメリカハワイ州
Year and Date
2010-09-25
Related Report
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