Establishment of radio sensitivity assay with a novel primary culture system from cancer patients

• Project/Area Number: 22791240
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: ENDO Hiroko 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), 研究所, 研究員 (20359300)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 放射線腫瘍学 / 癌細胞初代培養 / 放射線感受性試験

Research Abstract

We reported a novel primary culture system, Cancer Tissue-Originated S heroid (CTOS) method. In the present study, we tried to establish the radio sensitivity assay using CTOSs from lung cancer patients. CTOSs from different patients showed a variety of radiosensitivitiy, which reflected the therapeutic effect in mice tunlors. CTOS would be applicable for predicting individual radio sensitivity.

Research Products

• [Journal Article] Spheroid Culture of Primary Lung Cancer Cells with Neuregulin 1/HER3 Pathway Activation (2013)
  • Author(s): Endo H, Okami J, Okuyama H, Kumagai T, Harada J, Kondo J, Takehara T, Nishizawa Y, Imamura F, Higashiyama M, and Inoue M
  • Journal Title: Joumal of Thoracic Oncology Volume: 2 Pages: 131-9
  • Peer Reviewed
• [Journal Article] Involvement of heregulin/HER3 in the primary culture of human urothelial cancer (2013)
  • Author(s): Okuyama H, Yoshida T, Endo H, Nakayama M,Nonomura N, Nishimura K, and Inoue M
  • Journal Title: J.Urol Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Spheroid Culture of Primary Lung Cancer Cells with Neuregulin 1/HER3 Pathway Activation (2013)
  • Author(s): Hiroko Endo
  • Journal Title: Journal of Thoracic Oncology Volume: 8 Issue: 2 Pages: 131-139
  • DOI: 10.1097/jto.0b013e3182779ccf
  • Peer Reviewed
• [Journal Article] Involvement of Heregulin/HER3 in the Primary Culture of Human Urothelial Cancer (2013)
  • Author(s): Hiroaki Okuyama
  • Journal Title: Journal of Urology Volume: Epub ahead of print Issue: 1 Pages: 302-310
  • DOI: 10.1016/j.juro.2012.12.106
  • Peer Reviewed
• [Journal Article] Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from COlOreCtal CanCer (2011)
  • Author(s): Kondo J, Endo H, Okuyama H, Ishikawa O, Iishi H, Tsujii M, Ohue M, and Inoue M
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 15 Pages: 6235-40
  • DOI: 10.1073/pnas.1015938108
  • Peer Reviewed
• [Journal Article] Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer (2011)
  • Author(s): Jumpei Kondo
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 6235-6240
  • Peer Reviewed
• [Presentation] Hypoxia-induced tumor dormancy promotes cell survival through supPression of energy metabolism (2012)
  • Author(s): 遠藤洋子
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-20
• [Presentation] 低酸素によるがん細胞休眠状態の誘導 (2011)
  • Author(s): 遠藤洋子
  • Organizer: 第9回がんとハイポキシア研究会
  • Place of Presentation: 学習院大学目白キャンパス
  • Year and Date: 2011-11-26
• [Presentation] Prediction of therapeutic effect of EGFR TKI on lung cancer by a novel primary culture system (2011)
  • Author(s): 遠藤洋子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] A novel primary culture system of cancer cells from lung cancer patients (2010)
  • Author(s): 遠藤洋子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-24
• [Presentation] Hypoxia-induced tumor dormancy promotes cell survival through suppression of energy metabolism
  • Author(s): 遠藤 洋子
  • Organizer: 日本癌学会
  • Place of Presentation: ロイトン札幌
• [Presentation] Analysis of radiosensitivity mechamisms in cervical cancer CTOS
  • Author(s): 中嶋 綾
  • Organizer: 日本癌学会
  • Place of Presentation: ロイトン札幌
• [Remarks] 大阪府立成人病センター ホームページに掲載