| Project/Area Number |
22791242
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
SAITO Yukihiro 旭川医科大学, 医学部, 特任助教 (80540583)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 実験外科学 / 腫瘍血管新生 / 腫瘍リンパ管新生 / 癌幹細胞 / CSDA / アポトーシス / 癌 / 遺伝子 / 血管新生 / リンパ管新生 |
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| Research Abstract |
【Objective】Our objective was to investigate the effect of cold shock domain protein A (CSDA) on carcinogenesis and secretions of angiogenic and lymphangiogenic factors from tumor cells. 【Results】In human skeletal muscle cells (SkMC), CSDA was up-regulated during hypoxia when cells were damaged and apoptosis was induced. CSDA expression could up-regulate the c-fos and VEGF promoter activity on cancer cell lines, CT26, LLC, and AZL5G, but these results were inconsistent with previous reports. We hypothesized that CSDA expresses on cancer non-stem cells and CSDA can control the secretions angiogenic and lymphangiogenic factors from cancer stem cells. We tried to sort the cancer cell line, 4T1, and only 4% cells were recognized as the cancer stem cells.【Conclusion】Given with our in vitro data, we assessed novel hypothesis. Further experiments were required to confirm our concept.
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