The challenge to the liver transplantation by a fatty liver.- Treatment strategy for inflammation and coagulation by ischemia reperfusion injury.

• Project/Area Number: 22791247
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Mie University
• Principal Investigator: KISHIWADA MASASHI 三重大学, 医学部附属病院, 講師 (40501961)
• Project Period (FY): 2010-10-20 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 凝固・抗凝固因子 / 肝虚血再環流障害 / 生体肝移植 / マージナルドナー / ADAMS13 / TPO / 血小板 / 可溶性トロンボモジュリン(rhsTM) / ADANMSTS13 / VWFpp / 虚血再還流障害 / トロンボモジュリン / 脂肪肝 / ADAMTS13 / 血栓性微小血管障害症 / 虚血再環流 / トロンボモジュリン(TM) / 凝固因子 / アダムTS13 / ドナー肝重量 / 肝移植 / 活性化プロテインC / プロテインS / プロテインC

Research Abstract

1)In the rat liver ischemia reperfusion model , the hepatic protection effect of recombination human soluble thrombomodulin (rhsTM) was examined.
In the rhsTM group, liver enzymeserum, ED-1, TUNEL , and PCNA immunostaining reveal effect of rhsTM administration.
2)We examined coagulation and anti-anticoagulation factor in living donor liver transplantation patient. The blood platelet low value (100,000 or less) of continuation was a prognostic factor after liver transplantation. To determine risk factors associated with prolonged thrombocytopenia following LDLT, with a focus on the activity of ADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs member 13) and the influence of splenectomy.Prolonged thrombocytopenia after LDLT was associated with not only a decrease in ADAMTS13 due to sinusoidal endothelial cell injury, but also low TPO production due to hepatocyte dysfunction, irrespective of history of splenectomy.

Research Products

• [Journal Article] Thrombomodulin administration attenuates ischemia-reperfusion injury of the remnant liver after 70% hepatectomy in rats : simulated model of small-for-size graft in living donor liver transplantation (2014)
  • Author(s): Tanemura A, Kuriyama N, Azumi Y, Ohsawa I, Kishiwada M, Mizuno S, Usui M, Sakurai H, Tabata M, Isaji S.
  • Journal Title: Transplant Proc. Volume: 46 Pages: 1107-1111
  • Peer Reviewed
• [Journal Article] Prolonged thrombocytopenia after living donor liver transplantation is a strong prognostic predictor irrespective of history of splenectomy : the significance of ADAMTS13 and graft function (2014)
  • Author(s): Nobuoka Y, Wada H, Mizuno S, Kishiwada M, Usui M, Sakurai H, Tabata M, Kobayashi T, Nobori T, Uemoto S, Isaji S.
  • Journal Title: Int J Hematol Volume: 99 Pages: 418-428
  • Peer Reviewed
• [Journal Article] Prolonged thrombocytopenia after living donor liver transplantation is a strong prognostic predictor irrespective of history of splenectomy: the significance of ADAMTS13 and graft function. (2014)
  • Author(s): Nobuoka Y1, Wada H, Mizuno S, Kishiwada M, Usui M, Sakurai H, Tabata M, Kobayashi T, Nobori T, Uemoto S, Isaji S.
  • Journal Title: Int J Hematol. Volume: 99 Issue: 4 Pages: 418-428
  • DOI: 10.1007/s12185-014-1543-9
  • Peer Reviewed
• [Journal Article] Thrombomodulin administration attenuates ischemoa-reperfusion injury of the remnant liver after 70% hepatectomy in Rats: simulated model of small-for-size graft in living donor liver transplantation (2014)
  • Author(s): Tanemura A, Kuriyama N, Azumi I, Ohsawa M, Kishiwada M, Mizuno S, Usui M, Sakurai H, Tabata M, Isaji S.
  • Journal Title: Transplantation proceedings Volume: XX
  • Peer Reviewed
• [Journal Article] Borderline resectable膵癌とは? (2011)
  • Author(s): 岸和田昌之
  • Journal Title: 胆と膵 Volume: 32 Pages: 571-577
  • Peer Reviewed
• [Journal Article] The cytoprotective effects of addition of activated protein C into preservation solution on small-for-size grafts in rats (2010)
  • Author(s): 栗山直久
  • Journal Title: Liver Transplantation Volume: 16 Pages: 1-11
  • Peer Reviewed
• [Presentation] Thrombomodulin administration attenuates ischemia-reperfusion injury of the remnant liver after 70% hepatectomy in rats : simulated model of small-for-size graft in living donor liver transplantation (2013)
  • Author(s): Tanemura A, Kuriyama N, Azumi Y, Ohsawa I, Kishiwada M , Mizuno S, Usui M, Sakurai H, Tabata M, Isaji S.
  • Organizer: 第13回アジア移植学会議
  • Place of Presentation: 京都
  • Year and Date: 2013-09-04
• [Presentation] 生体肝移植術後血小板値はなぜ低下するのか? -ADAMTS13の変動から見たグラフト肝生体肝移植における予後予測-ADAMSTS13, VWFpp, TM, Tenascin-Cに着目して, 微小循環障害と予後の観点から (2013)
  • Author(s): 高橋直樹, 臼井 正信, 種村 彰洋, 栗山直久, 水野 修吾, 安積良紀, 岸和田 昌之,大澤 一郎, 櫻井 洋至, 田端 正己, 伊佐地 秀司, 和田英夫, 吉田利通
  • Organizer: 第31回日本肝移植研究会
  • Place of Presentation: 熊本
  • Year and Date: 2013-07-04
• [Presentation] Behavior of ADAMTS13 and Von Willebrand factor levels in patients after living donor liver transplantation (2013)
  • Author(s): Takahashi N, Wada H , Usui M, Iizawa Y, Sato R, Fujii T, Tanemura A, Kuriyama N, Azumi Y, Osawa I, Kishiwada M, Mizuno S, Sakurai H, Tabata M, Isaji S
  • Organizer: American transplant congress
  • Place of Presentation: シアトル,アメリカ
  • Year and Date: 2013-05-20
• [Presentation] 生体肝移植を想定した, ラット肝切除後残存肝虚血再還流障害におけるトロンボモジュリンの肝保護効果について (2013)
  • Author(s): 種村 彰洋, 栗山 直久, 安積 良紀, 大澤 一郎, 岸和田 昌之,水野 修吾, 臼井 正信, 櫻井 洋至, 田端 正己, 伊佐地 秀司
  • Organizer: 第113回日本外科学会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-05
• [Presentation] 生体肝移植グラフトを想定した、ラット70％肝切除後残存肝虚血再還流障害におけるトロンボモジュリンの肝保護効果について (2013)
  • Author(s): 種村彰洋、栗山直久、安積良紀、大澤一郎、岸和田昌之、水野修吾、臼井正信、櫻井洋至、田端正己、伊佐地秀司
  • Organizer: 第113回日本外科学会
  • Place of Presentation: 博多
• [Presentation] 脾摘出後肝硬変患者にはなぜ門脈血栓が高率に発生するのか？ ADAMATS13に着目して (2013)
  • Author(s): 高橋直樹、臼井正信、安積良紀、栗山直久、大澤一郎、岸和田昌之、水野修吾、櫻井洋至、田端正己、伊佐地秀司
  • Organizer: 第25回日本肝胆膵外科学会学術集会
  • Place of Presentation: 宇都宮
• [Presentation] 生体肝移植において高齢ドナー肝がドナー, レシピエントに与える影響 ～術後肝再生, ADAMTS13の推移～ (2012)
  • Author(s): 種村 彰洋, 水野 修吾, 栗山 直久, 大澤 一郎, 岸和田 昌之, 濱田賢司, 臼井 正信, 櫻井 洋至, 田端 正己, 伊佐地 秀司
  • Organizer: 第67回日本消化器外科学会集会
  • Place of Presentation: 富山
  • Year and Date: 2012-07-18
• [Presentation] 生体肝移植周術期における深在性真菌感染に対する早期診断と予防-真菌PCRと周術期経腸栄養に注目して (2011)
  • Author(s): 岸和田昌之
  • Organizer: 第24回日本外科感染症学会総会
  • Place of Presentation: 三重
  • Year and Date: 2011-12-02
• [Presentation] 生体肝移植術後血小板値はなぜ低下するのか? ADAMTS13の変動から見たグラフト肝微小循環障害と予後の観点から (2011)
  • Author(s): 信岡祐, 水野修吾, 和田秀夫, 大澤一郎, 岸和田昌之, 濱田賢司, 臼井正信, 櫻井洋至, 田端正己, 伊佐地秀司
  • Organizer: 第23回日本肝胆膵外科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] 進行膵癌100例に対する術前化学放射線療法の治療成績-NCCNガイドラインのresectabilityに注目して (2011)
  • Author(s): 岸和田昌之
  • Organizer: 第23回肝胆膵外科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-08