Investigation to determine a mechanism for T cell regulatory effects induced by treatment with high-dose immunoglobulin injection after organ transplantation.

• Project/Area Number: 22791253
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Hiroshima University
• Principal Investigator: TANAKA Yuka 広島大学, 大学院・医歯薬学総合研究科, 助教 (90432666)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 免疫グロブリン / 免疫寛容 / 臓器移植

Research Abstract

In the present study, we aimed to determine a mechanism of T cell regulatory effects induced by treatment with high-dose immunoglobulin injection after organ transplantation. By use of human lymphocyte reaction assay, we have proven that the Fc-part in IgG is responsible for the inhibiting effects on alloreactive T cells. We found that Peptide 289 inhibited proliferation of alloreactive T cells. However, its inhibitory extent was not comparable to conventional immunosuppressants. In addition, we developed a novel method to analyze STAT-signaling in alloreactive T cells in vitro.

Research Products

• [Journal Article] Optimization of immunosuppressive therapy based on amultiparametric mixed lymphocyte reaction assay reduces infectious complications and mortality in living donorliver transplant recipients (2012)
  • Author(s): Tanaka Y, Tashiro H, Onoe T, Ide K, Ishiyama K, Ohdan H
  • Journal Title: TransplantProc Volume: 44巻 Pages: 555-559
  • Peer Reviewed
• [Journal Article] Optimization of immunosuppressive therapy based on a multiparametric mixed lymphocyte reaction assay reduces infectious complications and mortality in living donor liver transplant recipients (2012)
  • Author(s): Tanaka Y, Tashiro H, Onoe T, Ide K, Ishiyama K, Ohdan H
  • Journal Title: Transplant Proc Volume: 44 Issue: 2 Pages: 555-559
  • DOI: 10.1016/j.transproceed.2012.01.038
  • Peer Reviewed
• [Journal Article] 高力価HBs抗体含有免疫グロブリン大量投与のアロ免疫応答に対する影響 (2011)
  • Author(s): 田中友加、大段秀樹
  • Journal Title: 今日の移植 Volume: 24巻 Pages: 103-107
• [Presentation] アロ応答T細胞のサイトイカイン産生能とSTATシグナル解析による分子標的免疫モニタリング法の開発 (2011)
  • Author(s): 田中友加、荒木慧、大段秀樹
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-06
• [Presentation] アロ応答T細胞のサイトイカイン産生能とSTATシグナル解析による分子標的免疫モニタリング法の開発 (2011)
  • Author(s): 田中友加、荒木慧、大段秀樹
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-10-06
• [Presentation] A novel method for intracellular profilingof STAT activation pattern in T cellsresponding allostimulation (2011)
  • Author(s): Kei Araki, Yuka Tanaka, Hideki Ohdan
  • Organizer: The 12th Congress of the Asian Society ofTransplantation
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-09-26
• [Presentation] Tailoring immunosup pressivetherapy on the basis of immune monitoringby a multiparametric mixed lymphocy tereaction assay reduces infectiousco mplications and mortality inliving-donor liver transplantation (2011)
  • Author(s): Yuka Tanaka, Hirotaka Tashiro, KentaroIde, Takashi Onoe, Kohei Ishiyama, HidekiOhdan
  • Organizer: The12th Congress of the Asian Society ofTransplantation
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-09-26
• [Presentation] A novel method for intracellular profiling of STAT activation pattern in T cells responding allostimulation (2011)
  • Author(s): Kei Araki, Yuka Tanaka, Hideki Ohdan
  • Organizer: The 12th Congress of the Asian Society of Transp lantation
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-09-26
• [Presentation] Tailoring immunosuppressive therapy on the basis of immune monitoring by a multiparametric mixed Iymphocyte reaction assay reduces infectious complications and mortality in living-donor liver transplantation (2011)
  • Author(s): Yuka Tanaka, Hirotaka Tashiro, Kentaro Ide, Takashi Onoe, Kohei Ishiyama, Hideki Ohdan
  • Organizer: The 12th Congress of the Asian Society of Transplantation.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-09-26
• [Presentation] 成人生体肝移植における免疫監視下での免疫抑制剤の最小化:Operational toleranceの誘導 (2011)
  • Author(s): 田中友加、井手健太郎、尾上隆司、石山宏平、田代裕尊、大段秀樹
  • Organizer: 第29回日本肝移植研究会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-07-23
• [Presentation] 肝臓移植後における自然免疫細胞移入と獲得免疫抑制の最適化による生体防御能を保持した免疫抑制療法 (2010)
  • Author(s): 田中友加、井手健太郎、尾上隆司、番匠谷将孝、五十嵐友香、田澤宏文、MarlenDoskali, Nabin Basnet, 田代裕尊、大段秀樹
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] 肝臓移植後における自然免疫細胞移入と獲得免疫抑制の最適化による生体防御能を保持した免疫抑制療法 (2010)
  • Author(s): 田中友加、井手健太郎、尾上隆司、番匠谷将孝、五十嵐友香、田澤宏文、Marlen Doskali, Nabin Basnet, 田代裕尊、大段秀樹
  • Organizer: 第46回 日本移植学会総会
  • Place of Presentation: みやこめっせ、京都
  • Year and Date: 2010-10-21
• [Presentation] Optimization of immunosup pressive the rapy on the basis of immune monitoringby a multipara metric mixed lymphocy tereaction assay reduces in fectiouscomp lications and mortality inliving-donor liver transplantation (2010)
  • Author(s): Yuka Tanaka, Hirotaka Tashiro, HidekiOhdan
  • Organizer: American Transplant Congress2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-05-04
• [Presentation] Optimization of immunosuppressive therapy on the basis of immune monitoring by a multiparametric mixed lymphocyte reaction assay reduces infectious complications and mortality in living-donor liver transplantation. (2010)
  • Author(s): Yuka Tanaka, Hirotaka Tashiro, Hideki Ohdan
  • Organizer: American Transplant Congress 2010.
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2010-05-04