Establish of Mesenchymal stem cell transplantation therapy for aplastic anemia animal

• Project/Area Number: 22791259
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Keio University
• Principal Investigator: SUZUKI Sadafumi 慶應義塾大学, 医学部, 研究員 (70465003)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 移植外科学 / 幹細胞移植 / 間葉系幹細胞 / 造血幹細胞 / 貧血 / 再生医療

Research Abstract

In this experiment, the goal is to establish a new therapy model for aplastic anemia. Our theory is that the transplanted Mesenchymal stem cell to disease model mice can reconstitute the niche cell, which can cure aplastic anemia.
At first, I analyzed Mesenchymal stem cell of Sca-1 knock out mouse.
I observed decrease of colony number as they get older.
Next, I did an experiment on Mesenchymal stem cell transplantation, but there is a problem that engraftment ratio is very low.
However, though there are individual differences, I observed the improvement of engraftment ratio with irradiation and administering of Substance P before the transplantation.

Research Products

• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Niibe K, Kawamura Y, Araki D, Morikawa S, Miura K, Suzuki S, Shimmura S, Sunabori T, Mabuchi Y, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: PLoS One Volume: 6(3)
  • Peer Reviewed
• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Niibe K
  • Journal Title: PLoS One Volume: 6 Issue: 3 Pages: e17610-e17610
  • DOI: 10.1371/journal.pone.0017610
  • Peer Reviewed
• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Kunimichi Niibe, Sadafumi Suzuki, et al.
  • Journal Title: Plos ONE Volume: 6(3)
  • Peer Reviewed
• [Journal Article] Inhibition of Abcg2 transporter on primitive hematopoietic stem cells by All-trans retinoic acid increases sensitivity to anthracycline (2010)
  • Author(s): Lein L, Nagai Y, Mabuchi Y, Suzuki S, Morikawa S, Matsuzaki Y
  • Journal Title: Inflammation and Regeneration Volume: 32(2) Pages: 722-31
  • NAID: 10026094290
  • Peer Reviewed
• [Journal Article] The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice (2010)
  • Author(s): Ruji Okazaki, Sadafumi Suzuki, et al.
  • Journal Title: Int J Radiat Biol. Volume: 86(11) Pages: 927-34
  • Peer Reviewed
• [Presentation] 眼慢性移植片対宿主病の発症機構におけるドナー間葉系幹細胞の役割 (2011)
  • Author(s): 小川葉子
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 東京都:東京国際フォーラム
  • Year and Date: 2011-05-13
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Yo Mabuchi, Sadafumi Suzuki
  • Organizer: International Society for Stem Cell Research (ISSCR), 8^<th> ANNUAL MEETING
  • Place of Presentation: USA・San Francisco
  • Year and Date: 2010-06-17
• [Presentation] PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLS ELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL COMPARTMENT (2010)
  • Author(s): Yo Mabuchi, Sadafumi Suzuki
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 兵庫・淡路島
  • Year and Date: 2010-05-13
• [Remarks]
  • URL: http://www.okano-lab.com/
• [Remarks]
  • URL: http://www.okano-lab.com/
• [Remarks]
  • URL: http://www.okano-lab.com/