The establishment of induction method from iPs cells to hepatocyte:For cell transplantation
| Project/Area Number |
22791275
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
ISHII Takamichi 京都大学, 大学院・医学研究科 肝胆膵移植外科分野, 助教 (70456789)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 肝臓外科学 / iPS 細胞 / 細胞移植治療 / 肝幹細胞 / iPS細胞 |
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| Research Abstract |
Recently, orthotopic liver transplantation is unique and the most efficient treatment for end-stage liver disease. But, the therapy has the issues of donor shortage and immune rejection. Then, a hepatocyte transplantation is listed as a alternative therapy for liver transplantation, we investigated hepatocyte transplantation.First, we selected iPS cells and mesenchymal stem cells as a cell source, and we tried and succeeded to induce differentiation of human induced pluripotent stem cells and human mesenchymal stem cells to hepatocyte like cells in vitro.In particular, the cells which was provided with extra cellular matrix and cytokines, expressed albumin and other hepatocyte marker in RT -PCR and immunohistochemistry. We transplanted these hepatocyte like cells into livers of SCID rats. In rats which were transplanted hepatocyte like cells derived from mesenchymal stem cells, we detected the albumin positive cells in the liver, in some of those rats, the serum level of human albumin increased.And then, in rats which were transplanted hepatocyte like cells derived from iPS cells, the albumin positive cells were detected in the liver .This result indicated that we can use iPS cells and mesenchymal stem cells as a cell source of hepatocyte transplantation.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage2012
Author(s)
Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
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Journal Title
Cell Transplant
Volume: 21(11)
Issue: 11
Pages: 2351-62
DOI
Related Report
Peer Reviewed
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[Journal Article] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers2010
Author(s)
Ishii T, Yasuchika K, Fukumitsu K, Kawamoto T, Kawamura-Saitoh M, Amagai Y, Ikai I, Uemoto S, Kawase E, Suemori H, Nakatsuji N
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Journal Title
Cell Tissue Res
Volume: 339(3)
Issue: 3
Pages: 505-12
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 大量肝切除後の急性肝不全に対する、肝細胞自家移植を用いた治療戦略2012
Author(s)
喜多貞彦,石井隆道,小木曾 聡,片山外大,河合隆之,安田勝太郎,上村 良,梶原正俊,波多野悦朗,落合孝広,上本伸二
Organizer
第39回日本臓器保存生物医学会
Place of Presentation
福島
Year and Date
2012-11-17
Related Report
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[Presentation] In vitro differentiation of human embryonic stem cells into functional hepatocytes using a mesenchymal cell line derived from murine fetal livers2010
Author(s)
Takamichi Ishii, Kentaro Yasuchika, Ken Fukumitsu, Kawamoto T, Kawamura-Saitoh M, Amagai Y, Iwao Ikai , Shinji Uemoto, Kawase E, Hirofumi Suemori, Norio Nakatsuji
Organizer
45thEASL
Place of Presentation
(Vienna, Austria
Year and Date
2010-04-11
Related Report
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