Analysis of the role of transcriptional factor Prox1 using genetically modified mice

• Project/Area Number: 22791277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: OKOSHI Kae 京都大学, 医学研究科, 特定病院助教 (90534355)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 癌 / 遺伝子

Research Abstract

Eleven types of ES clones were produced by gene transfer and three chimeras were obtained. By 2 times mating with FLP mice, five male and five female prox1+/-mice were obtained and they were confirmed by genotyping. We succeeded to prepare prox1-/-mice by mating prox1+/-mice. We investigated the role of Prox1 in the drug-induced esophageal cancer model using NMBA(N-nitrosomethylbenzylamine) under lack of the expression of Prox1 by infection of the Adeno-Cre.