Development of new efficient cancer-specific artificial viral transduction system

• Project/Area Number: 22791289
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: MANABE Tatsuya 九州大学, 医学研究院, 助教 (60546464)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膵癌 / 人工ウイルス

Research Abstract

Through the study of endocytosis pathway in pancreatic cancer, we found that adenovirus transgene expression has been declining which had been higher expression level of integrinβ3 thanβ5. In addition, we can encapsulate gemcitabine and siRNA of integrinβ3 to the protein nanocapsule Mj285(artificial virus) derived from the small heat shock protein produced by originally developed archaea Methanococcusjannaschii. Furthermore we can bind the virus to the antibody MUC1 by amide bond bioconjugation.

Research Products

• [Journal Article] Predicting the chemosensitivity of pancreatic cancer cells by quantifying the expression levels of genes associated with the metabolism of gemcitabine and 5-fluorouracil (2011)
  • Author(s): Kurata N, Fujita H, Ohuchida K, Mizumoto K, Mahawithitwong P, Sakai H, Onimaru M, Manabe T, Ohtsuka T, Tanaka M.
  • Journal Title: Int J Oncol Volume: 39(2) Pages: 473-82
  • Peer Reviewed
• [Journal Article] High EGFR mRNA expression is a prognostic factor for reduced survival in pancreatic cancer after gemcitabine-based adjuvant chemotherapy (2011)
  • Author(s): Fujita H, Ohuchida K, Mizumoto K, Itaba S, Ito T, Nakata K, Yu J, Kayashima T, Hayashi A, Souzaki R, Tajiri T, Onimaru M, Manabe T, Ohtsuka T, Tanaka M
  • Journal Title: Int J Oncol Volume: 38(3) Pages: 629-41
  • Peer Reviewed
• [Journal Article] Gene expression levels as predictive markers of outcome in pancreatic cancer after gemcitabine-based adjuvant chemotherapy (2010)
  • Author(s): Fujita H, Ohuchida K, Mizumoto K, Itaba S, Ito T, Nakata K, Yu J, Kayashima T, Souzaki R, Tajiri T, Manabe T, Ohtsuka T, Tanaka M.
  • Journal Title: Neoplasia Volume: 12(10) Pages: 807-17
  • Peer Reviewed