Gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells

• Project/Area Number: 22791308
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Gifu University
• Principal Investigator: MATSUNO Yukihiro 岐阜大学, 医学部・附属病院, 助教 (10542409)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 遺伝子治療 / 血管リモデリング / 超音波 / ナノバブル / 平滑筋前駆細胞 / マイクロバブル

Research Abstract

We investigated gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells. In vitro study, green fluorescent protein(GFP) gene was transferred to COS-7 cells using sonoporation and the expression was confirmed by Western blotting and fluorescence microscopy. In vivo study, GFP gene was transferred to rat carotid artery and the gene expression was confirmed by fluorescence microscopy. We will prepare nano-bubbles and evaluate the further effects using a functional gene.