The prevention of MRSA prosthetic vascular graft infection by using a DNA vaccine

• Project/Area Number: 22791309
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Gifu University
• Principal Investigator: MIZUNO Yoshimasa 岐阜大学, 医学部・附属病院, 助教 (60397354)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: MRSA / DNAワクチン / 人工血管感染

Research Abstract

I constructed DNA vaccine for the MRSA, and made an MRSA serious infectious disease model(mediastinitis caused by MRSA) and MRSA prosthetic vascular graft infection model. I failed to construct DNA vaccine by the method of the David M. Therefore I changed the means to the method of Hu DL. The new experimental plan goes, but I do not reach the stable composition of DNA vaccine. On the other hand, the bacteriological maneuver improved and I completed an MRSA mediastinitis model. I have been making the biofilm formation prosthetic vascular graft. The prosthetic vascular graft model is established at our department. It is necessary to realize stable supply of the DNA vaccine.