Research on gene copy number and resistance to EGFR-TKI therapy using silver in situ hybridization(SISH) technology for non-small-cell lung cancer

• Project/Area Number: 22791323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: YOSHIDA Koichi 東京医科大学, 医学部, 助教 (00424490)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肺癌 / EGFR / EGFR-TKI / EGFR遺伝子変異 / 上皮成長因子受容体 / 分子標的治療 / 薬剤耐性 / IGF-1R / FISH / SISH / 耐性 / IGF1R / 耐性因子 / 化学療法 / 遺伝子増幅

Research Abstract

An experimental SISH probe designed by Ventana Medical Systems(Tucson, AZ) was used to evaluate IGF1R gene copy number on 10 non-small-cell lung cancer(NSCLC) cell lines. The mean number of IGF1R gene copies/nuclei per cell line was 2.26(range : 1.12-7.56). The median copy number was 2.11.Squamous cell carcinoma had 7.56 copy number compared to non-SCC histologies. IGF1R gene copy number detected by SISH is higher in Squamous cell carcinoma cell line than in other cell line of NSCLC.

Research Products

• [Journal Article] Pathological Vascular Invasion and Tumor Differentiation Predict Cancer Recurrence in Stage IA Non-Small-Cell Lung Cancer After Complete Surgical Resection (2012)
  • Author(s): Shimada Y, Saji H, Yoshida K, Kakihana M, Honda H, Nomura M, Usuda J, Kajiwara N, Ohira T, Ikeda N
  • Journal Title: J Thorac Oncol
• [Journal Article] EGFR protein expression in non-small cell lung cancer predicts response to an EGFR tyrosine kinase inhibitor-a novel antibody for immunohistochemistry or AQUA technology (2011)
  • Author(s): Mascaux C, Wynes MW, Kato Y, Tran C, Asuncion BR, Zhao JM, Gustavson M, Ranger-Moore J, Gaire F, Matsubayashi J, Nagao T, Yoshida K, Ohira T, Ikeda N, Hirsch FR
  • Journal Title: Clin Cancer Res Volume: 17(24) Pages: 7796-807
• [Journal Article] Early experience using the da Vinci Surgical System for the treatment of mediastinal tumors (2011)
  • Author(s): Kajiwara N, Taira M, Yoshida K, Hagiwara M, Kakihana M, Usuda J, Uchida O, Ohira T, Kawate N, Ikeda N
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 59(10) Pages: 693-8
  • NAID: 10030256402
• [Journal Article] Prognostic impact of number of resected and involved lymph nodes at complete resection on survival in non-small cell lung cancer (2011)
  • Author(s): Saji H, Tsuboi M, Yoshida K, Kato Y, Nomura M, Matsubayashi J, Nagao T, Kakihana M, Usuda J, Kajiwara N, Ohira T, Ikeda N
  • Journal Title: J Thorac Oncol Volume: 6(11) Pages: 1865-71
• [Journal Article] Novel epidermal growth factor receptor mutation-specific antibodies for non-small cell lung cancer : immunohistochemistry as a possible screening method for epidermal growth factor receptor mutations (2010)
  • Author(s): Kato Y, Peled N, Wynes MW, Yoshida K, Pardo M, Mascaux C, Ohira T, Tsuboi M, Matsubayashi J, Nagao T, Ikeda N, Hirsch FR
  • Journal Title: J Thorac Oncol Volume: 5(10) Pages: 1551-8
• [Presentation] The role of IGF-1R in EGFR TKI resistance in NSCLC using IHC and AQUA technology (2011)
  • Author(s): Y. Kato, C. Mascaux, M. W. Wynes, B. Reyna Asuncion, C. Tran, K. Yoshida, J. Matsubayashi, E. Nakajima, T. Ohira, T. Nagao, K. Furukawa, N. Ikeda, F. R. Hirsch
  • Organizer: ASCO Annual Meeting