Nuclear Morphometry for Cytologic Diagnosis in Adenocarcinoma of the Lung

• Project/Area Number: 22791327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Dokkyo Medical University (2011); Gunma Institute of Public Health and Environmental Sciences (2010)
• Principal Investigator: NAKAZATO Yoshimasa 獨協医科大学, 医学部, 准教授 (10372921)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 呼吸器外科学 / 肺腺癌 / 核グレード / 細胞診 / EGFR / k-ras

Research Abstract

The nuclear area of tumor cells(119.1um^2±64.4) in adenocarcinoma of the lung was significantly larger than that of normal cells(67.1um^2±17.8)(P<0.05). The ROC curve analysis showed that an NA cut-off level of 81.0um^2 had a sensitivity of 80% and specificity of 86% to detect malignant strictures. The nuclear area of tumor cells in EGFR mutant type(92.5um^2±19.6) was significantly smallar than that of tumor cells in EGFR wild type(125.3um^2±69.5)(P<0.05).

Research Products

• [Presentation] Smart Amp法を用いた微量細胞検体におけるEGFR遺伝子変異解析 (2011)
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
  • Year and Date: 2011-11-18
• [Presentation] SMAP法を用いた微量検体におけるEGFR遺伝子変異解析 (2011)
  • Author(s): 吉田勤
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
  • Year and Date: 2011-11-18
• [Presentation] SMAP法を用いた微量細胞検体における遺伝子変異解析の紹介 (2011)
  • Author(s): 神山晴美
  • Organizer: 第52回日本細胞診学会春期大会演題
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-22
• [Presentation] SMAP法を用いた気管支鏡下肺生検時の微量細胞検体におけるEGFR遺伝子変異解析 (2011)
  • Organizer: 第34回日本呼吸器内視鏡学会
  • Place of Presentation: 浜松
• [Presentation] SMAP法を用いた微量細胞検体における遺伝子変異解析 (2011)
  • Organizer: 第52回日本臨床細胞学会春期大会
  • Place of Presentation: 福岡
• [Presentation] 迅速細胞診を併用したEGFR遺伝子変異検出解析 (2010)
  • Organizer: 第48回日本癌治療学会学術集会
  • Place of Presentation: 京都