Research for personalized treatment according to the epigenetic profile of glioblastoma and glioma stem cell

• Project/Area Number: 22791334
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: The University of Tokyo
• Principal Investigator: SAITO Kuniaki 東京大学, 医学部附属病院, 助教 (50446564)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 膠芽腫 / エピジェネティクス / メチル化 / 網羅的解析 / CpG island methylator phenotype (CIMP) / Isocitrate dehydrogenase (IDH) / 腫瘍幹細胞 / CIMP / Isocitrate dehydrogenase / 膠芽種 / MGMT / 個別化治療

Research Abstract

We extracted DNA from the glioma samples including glioblastoma and performed a methylation analysis of DNA promoter region and genetic anlaysis such as mutation of IDH and 1p19q codeltion. Genome-wide DNA methylation analysis identified G-CIMP and its strong association with IDH mutation. We selected CIMP markers which were differentially methylated in CIMP positive gliomas. CIMP marker genes showed distinct patterns of specific histone modification, suggesting the possibility of personalized treatment for the new targets.

Research Products

• [Journal Article] Significance of IDH mutation varies with tumor histology,grade, and genetics in Japanese glioma patients. (2012)
  • Author(s): 武笠晃丈、高柳俊作、齊藤邦昭、柴原純二、田部井勇助、成田善孝、西川亮、植木敬介、斉藤延人.
  • Journal Title: Cancer Science Volume: 103(3) Issue: 3 Pages: 587-92
  • DOI: 10.1111/j.1349-7006.2011.02175.x
  • Peer Reviewed
• [Presentation] DNAメチル化網羅的解析により明らかとなったG-CIMPの特徴とそのsubgroup (2012)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第30回日本脳腫瘍学会学術集会
  • Place of Presentation: グランドプリンスホテル広島(広島)
  • Year and Date: 2012-11-26
• [Presentation] 神経膠腫における網羅的DNAメチル化プロファイル解析により明らかとなったG-CIMPの特徴 (2012)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第71回日本脳神経外科学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2012-10-17
• [Presentation] Identification of global methylation profiling and novel methylation markers in glioma by epigenomewide analysis (2012)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第71回日本癌学会学術集会
  • Place of Presentation: ロイトン札幌(札幌)
  • Year and Date: 2012-09-20
• [Presentation] Genome-wide analysis of methylation profiling in glioma using illumina450K methylation array(ポスタ ー ) (2012)
  • Author(s): 齊藤 邦昭
  • Organizer: The 17th Annual Scientific Meeting of the Society for Neuro-Oncology.
  • Place of Presentation: Washington D.C.(USA)
  • Year and Date: 2012-01-16
• [Presentation] Identification of global methylation profiling and novel methylation markers in glioma by epigenomewide analysis (2012)
  • Author(s): 齊藤邦昭
  • Organizer: 第71 回日本癌学会学術集会
  • Place of Presentation: ロイトン札幌(札幌)
• [Presentation] Genome-wide analysis of methylation profiling in glioma using illumina450K methylation array (2012)
  • Author(s): 齊藤邦昭
  • Organizer: The 17th Annual Scientific Meeting of the Society for Neuro-Oncology
  • Place of Presentation: Washington D.C.
• [Presentation] MGMT promoter methylation is associated with malignant transformation in WHO grade 2 diffuse astrocytoma. (2011)
  • Author(s): 齊藤 邦昭.
  • Organizer: The 16th Annual Scientific Meeting of the Society for Neuro-Oncology.
  • Place of Presentation: Orange County California,USA
  • Year and Date: 2011-11-19
• [Presentation] MGMT promoter methylation is associated with malignant transformation in WHO grade 2 diffuse astrocytoma. (2011)
  • Author(s): 齊藤邦昭
  • Organizer: The 16th Annual Scientific Meeting of the Society for Neuro-Oncology
  • Place of Presentation: Orange County, California
• [Presentation] 高齢者glioblastomaに対する放射線治療・temozolomide併用療法 (2010)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第28回 日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢プリンスホテル(軽井沢)
  • Year and Date: 2010-11-29
• [Presentation] 高齢者glioblastomaに対する放射線治療・temozolomide併用療法 (2010)
  • Author(s): 齊藤邦昭
  • Organizer: 第28回日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢プリンスホテルウエスト(長野県)
  • Year and Date: 2010-11-29
• [Presentation] MGMT promoter methylation is associated with malignant transformation in WHO grade 2 diffuse astrocytoma. (2010)
  • Author(s): 齊藤 邦昭.
  • Organizer: The 15th Annual Scientific Meeting of the Society for Neuro-Oncology.
  • Place of Presentation: Montreal,Canada
  • Year and Date: 2010-11-19
• [Presentation] MGMT promoter methylation is associated with malignant transformation in WHO grade 2 diffuse astrocytoma (2010)
  • Author(s): 齊藤邦昭
  • Organizer: The 15^<th> Annual Scientific Meeting of the society for Neuro-Oncology
  • Place of Presentation: カナダ・モントリオール
  • Year and Date: 2010-11-19
• [Presentation] 高齢者 glioblastoma に対する temozolomide 治療の有効性と安全性 (2010)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2010-10-29
• [Presentation] 高齢者glioblastomaに対するtemozolomide治療の有効性と安全性 (2010)
  • Author(s): 齊藤邦昭
  • Organizer: 第69回日本脳神経外科学会総会
  • Place of Presentation: 福岡国際会議場他(福岡県)
  • Year and Date: 2010-10-29
• [Presentation] WHO grade2,3,4のGliomaにおけるMGMTプロモーター領域のメチル化および予後の解析 (2010)
  • Author(s): 齊藤 邦昭.
  • Organizer: 第11回分子脳神経外科学会
  • Place of Presentation: 艮陵会館記念ホール(仙台)
  • Year and Date: 2010-08-28
• [Presentation] WHO grade2,3,4のGliomaにおけるMGMTプロモーター領域のメチル化および予後の解析 (2010)
  • Author(s): 齊藤邦昭
  • Organizer: 第11回分子脳神経外科学会
  • Place of Presentation: 艮陵会館記念ホール(宮城県)
  • Year and Date: 2010-08-28
• [Presentation] Prognostic significance of MGMT promoter methylation in patients with grade 2, 3, 4 glioma. (2010)
  • Organizer: 7th Meeting of Adian Society for Neuro-Oncology
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2010-06-12
• [Presentation] Prognostic significance of MGMT promoter methylation in patients with grade 2,3, and 4 glioma (2010)
  • Author(s): 齊藤邦昭
  • Organizer: 7^<th> Meeting of Asian Society for Neuro-Oncology
  • Place of Presentation: 韓国・ソウル
  • Year and Date: 2010-06-12
• [Remarks] 東京大学医学部脳神経外科研究内容