Transplantation of multiple type of the cells derived from olfactory mucosa for the recovery of spinal cord injury

• Project/Area Number: 22791342
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Osaka University
• Principal Investigator: ISHIHARA Masahiro 大阪大学, 医学系・研究科, 特任研究員 (00533803)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 神経再生 / 脊髄損傷 / 神経前駆細胞 / 嗅神経鞘細胞

Research Abstract

The olfactory mucosal cells(OMCs) contain neural progenitor cells(NPCs) and olfactory ensheathing cells(OECs), both of which have been reported to be effective in neuronal and axonal regeneration. We measured neurotrophins production of OMCs and revealed OMCs mainly produced NT-3 in this system. Because anti-neurotrophin antibody cocktail drastically reduced axon-promoting effects of the OMCs, NT-3 production is the key factor of axon-promoting effects of OMCs. Extending axons from cortex were mainly observed in OECs. Another possibility is that NPC in the OMCs matured to neurons. Neural cells constituted 14% of OMCs. We are now examining to identify the interaction between neurons from cotex and from OMCs.

Research Products

• [Journal Article] Disuse muscle atrophy exacerbates motor neuronal degeneration caudal to the site of spinal cord injury (2012)
  • Author(s): Ohnishi Y, Iwatsuki K, Shinzawa K, Nakai Y, Ishihara M, Yoshimine T
  • Journal Title: Neuroreport Volume: 23 Pages: 157-161
  • Peer Reviewed
• [Journal Article] A new three-dimensional axonal outgrowth assay for central nervous system regeneration (2011)
  • Author(s): M. Ishihara et al
  • Journal Title: Journal of Neuroscience Methods Volume: 198 Pages: 181-186
  • Peer Reviewed
• [Journal Article] Percutaneous Diode Laser Irradiation for Lumber Discogenic Pain : A Clinical Study (2011)
  • Author(s): Iwatsuki K, Yoshimine T, Umegaki M, Yoshimura K, Ohnishi Y, Ishihara M, Moriwaki T
  • Journal Title: Photomedicine and Laser Surgery Volume: 29 Pages: 459-462
  • Peer Reviewed
• [Journal Article] Histopathological Study of Spinal Meningioma Originating from Arachnoid Villi : A Case Report (2011)
  • Author(s): Yu-ichiro Ohnishi, Koichi Iwatsuki, Eiichi Morii, Maki Kobayashi, Yumiko Hori, Takashi Moriwaki, Masahiro Ishihara, Kazunori Yoshimura, Masao Umegaki and Toshiki Yoshimine
  • Journal Title: Brain Tumor Phathol Volume: 28 Pages: 77-81
  • Peer Reviewed
• [Journal Article] Percutaneous Diode Laser Irradiation for Lumbar Discogenic Pain : A Clinical Study (2011)
  • Author(s): K.Iwatsuki, et al
  • Journal Title: Photomedicine and Laser Surgery Volume: 29 Pages: 459-463
  • Peer Reviewed
• [Journal Article] Histopathological Study of Spinal Meningioma Originating from Arachnoid Villi : A Case Report (2011)
  • Author(s): Ohnishi Y., et al
  • Journal Title: Brain Tumor Pathol Volume: 28 Issue: 1 Pages: 77-81
  • DOI: 10.1007/s10014-010-0003-3
  • Peer Reviewed
• [Presentation] Olfactory mucosal cells promote axonal regeneration in newly three-dimensional assay (2011)
  • Author(s): M Ishihara, et al
  • Organizer: Neuroscience 2011, SfN's 41st annual meeting
  • Place of Presentation: Washington D.C.(USA)
  • Year and Date: 2011-11-14
• [Presentation] 嗅粘膜由来細胞の持つ神経軸索再生効果についての検討～in vitro 3次元培養系を用いて～ (2011)
  • Author(s): 石原正浩
  • Organizer: 第69回脳神経外科学会総会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2011-10-27
• [Presentation] 嗅粘膜由来細胞の神経栄養因子分泌能およびその形態変化についての検討 (2011)
  • Author(s): 石原正浩
  • Organizer: 第70回脳神経外科学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-10-12
• [Presentation] 初代嗅粘膜由来細胞の神経栄養因子分泌能および形態変化についての検討 (2011)
  • Author(s): 石原正浩
  • Organizer: 第26回脊髄外科学会
  • Place of Presentation: 沼津市民文化センター
  • Year and Date: 2011-06-10
• [Presentation] Olfactory mucosal cells promote axonal regeneration in newly three-dimensional assay (2011)
  • Author(s): M Ishihara
  • Organizer: Neuroscience 2011, SfN' s 41st annual meeting : November 14^<th>
  • Place of Presentation: Washington D. C.(USA)
• [Presentation] 嗅粘膜由来細胞の持つ神経軸索再生効果についての検討～in vitro 3次元培養系を用いて～ (2010)
  • Author(s): 石原正浩
  • Organizer: 脳神経外科学会第69回学術総会
  • Place of Presentation: 福岡コンベンションセンター
  • Year and Date: 2010-10-27
• [Presentation] Olfactory mucosal cells promote axonal regeneration in a newly developed vitro model. (2010)
  • Author(s): Masahiro Ishihara
  • Organizer: Asia Pacific Symposium on Neural Regeneration
  • Place of Presentation: Yonsei University(Korea)
  • Year and Date: 2010-09-17
• [Presentation] Regeneration of cortical axons into the three-dimensional gel containing olfactory cells. (2010)
  • Author(s): Masahiro Ishihara
  • Organizer: 8^<th> International Society for Stem Cell Research
  • Place of Presentation: Moscon convention center(USA)
  • Year and Date: 2010-06-17
• [Presentation] 3次元ゲルアッセイ系を用いた嗅粘膜由来細胞の持つ神経軸索再生効果の検討 (2010)
  • Author(s): 石原正浩
  • Organizer: 第25回日本脊髄外科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2010-06-09
• [Presentation] 大脳皮質-Matrigel共培養アッセイ系を用いた嗅粘膜由来細胞の持つ神経軸索再生効果の検討 (2010)
  • Author(s): 石原正浩
  • Organizer: 第25回神経組織の成長・再生・移植研究会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2010-05-22
• [Presentation] Regeneration of cortical axons into the three-dimensional gel containing olfactory cells (2010)
  • Author(s): M Ishihara
  • Organizer: International Society for Stem Cell Research 8^<th> annual meeting 16^<th>
  • Place of Presentation: San Franscisco(USA)