Effect of Cholesterol on Sphingosylphosphorylcholine-Rho-kinase -mediated Cerebral Vasospasm
| Project/Area Number |
22791346
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
SHIRAO Satoshi 山口大学, 大学院・医学系研究科, 助教 (70448281)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ラフト / コレステロール / スフィンゴ脂質 / 脳血管攣縮 / 高脂血症 / Cranial Window法 |
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| Research Abstract |
Lipid rafts are cholesterol-enriched membrane microdomains that influence signal transduction. In this study, hypercholesterolemia increased vascular smooth muscle (VSM) cholesterol (but not PC, a major component of nonraft membranes) and sphingosylphosphorylcholine(SPC)-ROK-mediated VSM contraction. Depletion of VSM cholesterol (but not PC) byβ-cyclodextrin(β-CD) inhibited SPC-induced contraction. Treatment with β-CD decreased the mean % filipin III staining intensity of Human brain VSM cells. Depletion of VSM cholesterol by β -CD also decreased the raft marker ganglioside GM1 count per unit membrane length and inhibited SPC-induced contraction. These results indicate that cholesterol potentiates SPC-ROK-mediated contractions of importance in cerebral vasospasm and are compatible with a role for lipid rafts in this process.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Sphingosylphosphorylcholine-Rho-kinasemediated smooth muscle contraction in the rat basilar artery is regulated by the serum total cholesterol or vascular tissue cholesterol level: a mechanistic role for lipid rafts2011
Author(s)
Satoshi Shirao, Hiroshi Yoneda, Mizuya Shinoyama, Yoshihiko Maeda, Hiroyasu Koizumi, Hideyuki Ishihara, Fumiaki Oka, Hirokazu Sadahiro, Sadahiro Nomura, Koji Kajiwara, and Michiyasu Suzuki
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Journal Title
Related Report
Peer Reviewed
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