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Mechanisms underlying regulation of Wnt signaling pathway against cancer stem-and glioma cells

Research Project

Project/Area Number 22791348
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionThe University of Tokushima

Principal Investigator

MIZOBUCHI Yoshifumi  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (80547993)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords脳腫瘍 / REIC-Dkk3 / Wntシグナル / REIC-DKK-3 / 遺伝子治療
Research Abstract

Reduced expression in immortalized cells/ Dikkofp-3(REIC/ DKK3, REIC), a tumor suppressor gene, has been investigated in gene therapy studies in various cancers. Elsewhere we first demonstrated that REIC was down-regulated in glioblastoma(GBM) cells, and that its over-expression with plasmid vector drastically inhibited their growth via activation of intrinsic apoptotic pathway. However, whether adenovirus-mediated overexpression of REIC(Ad-REIC) contributes to anti-tumor effect in glioma remain unclear. We performed this study to assess the applicability of Ad-REIC in GBM and to verify the molecular mechanisms underlying anti-tumor effect of Ad-REIC We first document that Ad-REIC effectively inhibited the growth of GBM in a time-and dose dependent manner and induced apoptosis. We also found that Ad-REIC reduced expression of Wnt proteins and its receptor LRP6 but not ROR2 Our data suggest that interfering the activation of both canonical-and non-canonical Wnt signaling pathway via overexpression of REIC contributes at least partly to suppress cell survival in GBM.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (1 results)

  • [Journal Article] Severe head injuries during Judo practice]2011

    • Author(s)
      Nagahiro S, Mizobuchi Y, Hondo H, Kasuya H, Kamitani T, Shinbara Y, Nimura Y, Tomatsu T.
    • Journal Title

      No Shinkei Geka

      Volume: 39 Pages: 1139-47

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical results of boron neutron capture therapy(BNCT) for glioblastoma2011

    • Author(s)
      Kageji T, Mizobuchi Y, Nagahiro S, Nakagawa Y, Kumada H
    • Journal Title

      Appl Radiat Isot

      Volume: 69 Issue: 12 Pages: 1823-5

    • DOI

      10.1016/j.apradiso.2011.05.029

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-survivors of glioblatoma treated with boron neutron capture therapy(BNCT)2011

    • Author(s)
      Kageji T, Mizobuchi Y, Nagahiro S, Nakagawa Y, Kumada H
    • Journal Title

      Appl Radiat Isot

      Volume: 69 Issue: 12 Pages: 1800-2

    • DOI

      10.1016/j.apradiso.2011.03.021

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Practical management of botulinum toxin therapy2011

    • Author(s)
      Lim EC, Bhidayasiri R, Rosales RL, Kaji R.
    • Journal Title

      Introduction. Parkinsonism & related disorders

      Volume: 17 Issue: 2 Pages: 123-5

    • DOI

      10.1016/j.parkreldis.2010.11.013

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Akt2 and Akt3 play a pivotal role in malignant gliomas2011

    • Author(s)
      Mure H, et al
    • Journal Title

      Neuro Oncol

      Volume: 12 Pages: 221-232

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] REIC/Dkk-3 gene and protein regulate medulloblastoma cell growth2012

    • Author(s)
      Mizobuchi Y, et al
    • Organizer
      AANS 2012
    • Place of Presentation
      Miami, USA
    • Year and Date
      2012-04-15
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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