Elucidation of the mechanism of cancer by methylation of histone and methylated genes in malignant brain tumor

• Project/Area Number: 22791350
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Saga University
• Principal Investigator: NAKAHARA Yukiko 佐賀大学, 医学部, 助教 (50380770)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: エピジェネティクス / メチル化 / 膠芽腫 / EHMT1 / 悪性脳腫瘍 / ヒストン

Research Abstract

We examined the genetic and epigenetic aberration of EHMT1 gene in the several glioblastoma cell lines and clinical specimen. Genomic quantitative PCR was performed in glioblastioma cell lines, such as U87MG, T98G, U251MG, U118MG and A 172. None of all cell lines showed the loss of heterozygosity. We did not detect the mutation of the EHMT1 gene on genomic sequence. We also examine the expression levels of mRNA and protein of EHMT1 using quantitative RT-PCR and Western blotting method in glioblastoma cell lines and clinical specimen. The expression of mRNA and protein of EHMT1 did not decrease in those materials.