Functional analysis of MIF in brain tumor initiating cell

• Project/Area Number: 22791356
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Keio University
• Principal Investigator: FUKAYA Raita 慶應義塾大学, 医学部, 助教 (60348670)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脳腫瘍幹細胞 / グリオーマ / 脳腫瘍

Research Abstract

In this study, we performed the functional analysis of MIF in glioma and brain tumor stem cells(BTSCs). Knockdown of MIF suppressed the cell proliferation in glioma and the effect was dependent on p53 expression. MIF was shown to bind p53 and inhibit its functions as a tumor suppressor in glioma cells. Moreover, MIF knockdown inhibited not only in vitro cell growth, but also in vivo tumor growth in BTSCs. These results suggest that MIF can be a target for the treatment of BTSCs.

Research Products

• [Journal Article] Macrophage migration inhibitory factor(MIF) promotes cell survival and proliferation of neural stem/progenitor cells (2012)
  • Author(s): Ohta S, Misawa A, Fukaya R, Inoue S, Kanemura Y, Okano H, Kawakami Y, Toda M
  • Journal Title: J Cell Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] Macrophage migration inhibitory factor (MIF) promotes cell survival and proliferation of neural stem/progenitor cells (2012)
  • Author(s): Ohta S, Misawa A, Fukaya R, Inoue S, Kanemura Y, Okano H, Kawakami Y, Toda M
  • Journal Title: J Cell Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells (2011)
  • Author(s): Tabuse M, Ohta S, Ohashi Y, Fukaya R, Misawa A, Yoshida K, Kawase T, Saya H, Thirant C, Chneiweiss H, Matsuzaki Y, Okano H, Kawakami Y, Toda M
  • Journal Title: Molecular Cancer 10(60) online journal
  • Peer Reviewed
• [Journal Article] Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells (2011)
  • Author(s): Tabuse M, Ohta S, Ohashi Y, Fukaya R, Misawa A, Yoshida K, Kawase T, Saya H, Thirant C, Chneiweiss H, Matsuzaki Y, Okano H, Kawakami Y, Toda M
  • Journal Title: Mol Cancer Volume: 10 Issue: 1 Pages: 60-60
  • DOI: 10.1186/1476-4598-10-60
  • Peer Reviewed
• [Journal Article] Isolation of cancer stem-like cells from a side population of a human glioblastoma cell line, SK-MG-1 (2010)
  • Author(s): Fukaya R, Ohta S, Yamaguchi M, Fujii H, Kawakami Y, Kawase T, Toda M
  • Journal Title: Cancer Let Volume: 291(2) Pages: 150-157
  • Peer Reviewed
• [Presentation] ヒトグリオーマ及びグリオーマ癌幹細胞におけるHOXD9の機能解析 (2011)
  • Author(s): 田伏将尚、大多茂樹、大橋陽平、深谷雷太、三沢彩、吉田一成、河瀬斌、佐谷秀行、Thirant C、Chneiweiss H、松崎有未、岡野栄之、河上裕、戸田正博
  • Organizer: 第12回日本分子脳神経外科学会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-10-14
• [Presentation] Functional analysis of HOXD9 in human gliomas and glioma cancer-initiating cells (2011)
  • Author(s): 大多茂樹、田伏将尚、大橋陽平、深谷雷太、三沢彩、吉田一成、河瀬斌、佐谷秀行、Thirant C、Chneiweiss H、松崎有未、岡野栄之、河上裕、戸田正博
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光(山代温泉)(石川県)
  • Year and Date: 2011-09-27
• [Presentation] 脳腫瘍幹細胞・グリオーマ共通抗原の発現および機能解析 (2010)
  • Author(s): 戸田正博、田伏将尚、大多茂樹、深谷雷太、高橋里史、吉田一成
  • Organizer: 第28回日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢
  • Year and Date: 2010-11-29
• [Presentation] Brain tumor initiating cellとMIFの役割 (2010)
  • Author(s): 深谷雷太、大多茂樹、松崎有未、河上裕、岡野栄之、河瀬斌、吉田一成、戸田正博
  • Organizer: 日本脳神経外科学会第69回学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-28
• [Presentation] Brain tumor initiating cellとp53結合分子MIFの役割 (2010)
  • Author(s): 深谷雷太、大多茂樹、松崎有未、河上裕、岡野栄之、河瀬斌、吉田一成、戸田正博
  • Organizer: 第11回日本分子脳神経外科学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-08-27