N-Acetylglucosamine 6-O-Sulfotransferase-1 is crucialenzyme for 5D4-reactive keratin sulfate biosynthesis which elicits expression after spinal cord injury.
| Project/Area Number |
22791371
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
ANDO Kei 名古屋大学, 医学部附属病院, 医員 (40566973)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | GlcNAc6ST-1 / 脊髄損傷 / 脊髄腫瘍 / KSPG / 5D4 / KSGal6ST-1 |
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| Research Abstract |
：5D4-reactive KSPGs were decrease as aging in wild type mice CNS, and were loss of expression in GlcNAc6ST-1-/- mice. On the other hand, BCD4-reactive KSPGs were increased as aging in wild type and GlcNAc6ST- 1-/- mice CNS. The KS enzyme b3GlcNAcT-7 was also significantly down-regulated not only GlcNAc6ST-1 in CNS of GlcNAc6ST-1-/- mice compared to that of wild type mice. 5D4-reactive KSPGs were decreased for cell lysates of GlcNAc6ST-1 siRNA transfected BV2, although BCD4-reactive KSPGs were not decreased. The enzyme b3GlcNAcT-7 was significantly downregulated not only GlcNAc6ST-1 same as in vivo study. The enzyme significantly down-regulated was only b3GlcNAcT-7. If the ablation of 5D4-reactive KS except for BCD4-reactive KS is possible, better functional recovery may be obtained. Ourdata suggested that GlcNAc6ST-1 was important enzyme for 5D4-reactive KSPGs biosynthesis, b3GlcNAcT-7 for both 5D4 and BCD4-reactive KSPGs biosynthesis. The data was supported with in vitro study used transfected microglia cell line. Interestingly, GlcNAc6ST-1 may regulate b3GclNAcT-7 by decrease of own expression. GlcNAc6ST-1 specific down-regulation treatment may be the key in CNS injury.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Differentiation of Spinal Schwannomas and Myxopapillary Ependymomas: MR Imaging and Pathologic Features2013
Author(s)
Kei Ando, Imagama S, Ito Z, Hirano K, Tauchi R, Muramoto A, Matsui H, Matsumoto T, Sakai Y, Matsuyama Y, Ishiguro N
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Examination of the influence of ossification of the anterior longitudinal ligament on symptom progression and surgical outcome of ossification of the thoracic ligamentum flavum: a multicenter study2012
Author(s)
Kei Ando, Imagama S, Wakao N, Hirano K, Tauchi R, Muramoto A, Kato F, Yukawa Y, Kawakami N, Sato K, Matsubara Y, Kanemura T, Matsuyama Y, Ishiguro N
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Journal Title
J Neurosurg Spine
Volume: 16
Pages: 147-153
Related Report
Peer Reviewed
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