| Project/Area Number |
22791371
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
ANDO Kei 名古屋大学, 医学部附属病院, 医員 (40566973)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | GlcNAc6ST-1 / 脊髄損傷 / 脊髄腫瘍 / KSPG / 5D4 / KSGal6ST-1 |
|---|
| Research Abstract |
：5D4-reactive KSPGs were decrease as aging in wild type mice CNS, and were loss of expression in GlcNAc6ST-1-/- mice. On the other hand, BCD4-reactive KSPGs were increased as aging in wild type and GlcNAc6ST- 1-/- mice CNS. The KS enzyme b3GlcNAcT-7 was also significantly down-regulated not only GlcNAc6ST-1 in CNS of GlcNAc6ST-1-/- mice compared to that of wild type mice. 5D4-reactive KSPGs were decreased for cell lysates of GlcNAc6ST-1 siRNA transfected BV2, although BCD4-reactive KSPGs were not decreased. The enzyme b3GlcNAcT-7 was significantly downregulated not only GlcNAc6ST-1 same as in vivo study. The enzyme significantly down-regulated was only b3GlcNAcT-7. If the ablation of 5D4-reactive KS except for BCD4-reactive KS is possible, better functional recovery may be obtained. Ourdata suggested that GlcNAc6ST-1 was important enzyme for 5D4-reactive KSPGs biosynthesis, b3GlcNAcT-7 for both 5D4 and BCD4-reactive KSPGs biosynthesis. The data was supported with in vitro study used transfected microglia cell line. Interestingly, GlcNAc6ST-1 may regulate b3GclNAcT-7 by decrease of own expression. GlcNAc6ST-1 specific down-regulation treatment may be the key in CNS injury.
|
