Development of novel biomaterial for intervertebral disc repair

• Project/Area Number: 22791374
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: AKEDA Koji 三重大学, 医学部・附属病院, 講師 (20422826)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 脊椎 / 椎間板変性 / 生体材料 / プロテオグリカン / グリコサミノグリカン / 糖鎖 / 成長因子 / 家兎 / グリコサミノグリカ / 血小板

Research Abstract

Aggrecan(large proteoglycan) is one of the main extracellular matrix molecules of intervertebral disc(IVD). Gellan sulfate is a polysaccharide-biomaterial, whose chemical structure is similar to glycosaminoglycan. The purpose of this study was(1) to examine the effect of gellan sulfate(GS) on the extracellular matrix metabolism of bovine intervertebral disc(IVD) cells in vitro and(2) to evaluate the effect of injectable-GS on the progression of IVD degeneration in the rabbit IVD degeneration model. GS(32% sulfation rate) significantly stimulated the cell proliferation and proteoglycan synthesis of bovine IVD cells. Intradiscal injection of injectable-GS significantly inhibited the progression of disc height narrowing in the rabbit IVD degeneration model. Histological analysis showed that the injectable-GS had a high tissue affinity and increased the activation of matrix metabolism of rabbit IVD tissue.

Research Products

• [Journal Article] Acridine orange inhibits pulmonary metastasis of mouse osteosarcoma (2011)
  • Author(s): Satonaka H., Kusuzaki K., Akeda K., Tsujii M., Iino T., Uemura T., Matsubara T., Nakamura T., Asanuma K., Matsumine A., Sudo A
  • Journal Title: Anticancer Res Volume: 31 Pages: 4163-4168
  • Peer Reviewed
• [Journal Article] Upper airway obstruction associated with flexed cervical position after posterior occipitocervical fusion (2011)
  • Author(s): Tagawa T., Akeda K., Asanuma Y., Miyabe M., Arisaka H., Furuya M., Yoshida K., Sakuraba S
  • Journal Title: J Anesth Volume: 25 Pages: 120-122
  • NAID: 10029022064
  • Peer Reviewed
• [Journal Article] Distribution and role of tenascin-C in human osteoarthritic cartilage (2010)
  • Author(s): Nakoshi Y., Hasegawa M., Akeda K., Iino T., Sudo A., Yoshida T., Uchida A
  • Journal Title: J Orthop Sci Volume: 15 Pages: 666-673
  • NAID: 10027872151
  • Peer Reviewed
• [Journal Article] Transfection of NF-kappaB decoy oligodeoxynucleotide suppresses pulmonary metastasis by murine osteosarcoma (2010)
  • Author(s): ishimura A., Akeda K., Matsubara T., Kusuzaki K., Matsumine A., Masuda K., Gemba T., Uchida A., Sudo A
  • Journal Title: Cancer Gene Ther Volume: 18 Pages: 250-259
  • Peer Reviewed
• [Journal Article] Transfection of NF-κB decoy oligodeoxynucleotide suppresses pulmonary metastasis by murine osteosarcoma. (2010)
  • Author(s): Nishimura A, Akeda K, et al.
  • Journal Title: Cancer Gene Ther Volume: 18(4) Pages: 250-9
  • Peer Reviewed
• [Journal Article] Distribution and role of tenascin-C in human osteoarthritic cartilage. (2010)
  • Author(s): Nakoshi Y, Akeda K, et al.
  • Journal Title: J Orthop Sci Volume: 15(5) Pages: 666-73
  • NAID: 10027872151
  • Peer Reviewed
• [Journal Article] 転移性脊椎腫瘍術後に巨大な偽性髄膜瘤を生じた1例 (2010)
  • Author(s): 奥野一真、明田浩司, 他
  • Journal Title: 中部日本整形外科災害外科学会雑誌 Volume: 53(6) Pages: 1443-4
  • Peer Reviewed
• [Presentation] Biological Repair of Intervertebral Disc Degeneration Using Autologous Platelet-rich Plasma (2012)
  • Author(s): Koji Akeda
  • Organizer: 第15回脊椎と神経を語る会
  • Place of Presentation: ホテルオークラ東京(招待講演)
  • Year and Date: 2012-03-11
• [Presentation] Inhibitory Effect of NF-KB using Technique of Synthetic Double-Stranded Oligodeoxynucleotide for Tissue Necrosis Following Ischemia-Reperfusion Injury (2012)
  • Author(s): Uemura T, Tujii M, Akeda K, Satonaka H, Hori K, Iino T, Fujisawa K, Sudo A
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] Effect of Tenascin-C on the repair of full-thickness osteochondral defects of articular cartilage in rabbits (2012)
  • Author(s): Ikemura S, Hasegawa M, Iino T, Akeda K, Miyamoto K, Imanaka-Yoshida K, Yoshida T, Sudo A
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: San Erancisco
• [Presentation] Intradiscal Injection of Autologous Platelet-Rich-Plasma for the Treatment of Lumbar Disc Degeneration -Preliminary Prospective Clinical Trial for Discogenic Low Back Pain Patients (2012)
  • Author(s): Akeda K, Imanishi T, Ohishi K, Masuda K, Uchida A, Sakakibara T, Kasai Y, Sudo A
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] Effect of diminished flow in rabbit lumber arteries on intervertebral disc matrix changes : MRI T2-mapping and histological study (2012)
  • Author(s): Imanishi T, Akeda K, Obata S, Iino T, Yamada T, Sudo A
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] Acridine Orange Inhibits Pulmonary Metastasis and Invasion on Mouse Oseteosarcoma (2012)
  • Author(s): Satanaka H, Akeda K, Kusuzaki K, Tujii M, Iino T, Uemura T, Nakamura T, Matsubara T, Asanuma K, Matsumine A, Sudo A
  • Organizer: Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] 椎間板変性の病態と最新研究 (2011)
  • Author(s): 明田浩司
  • Organizer: 第11回海部整形外科医会総会
  • Year and Date: 2011-02-06
• [Presentation] 多血小板血漿由来血清を用いた生物学的椎間板治療-椎間板性疼痛患者に対する臨床トライアル- (2011)
  • Author(s): 明田浩司、今西隆夫、小畑秀司、中村知樹、大石晃嗣、舛田浩一、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 群馬県前橋市
• [Presentation] 多血小板血漿を用いた生物学的椎間板内治療-若中年者の椎間板性疼痛患者に対する臨床トライアル- (2011)
  • Author(s): 明田浩司、今西隆夫、大石晃嗣、舛田浩一、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第19回日本腰痛学会
  • Place of Presentation: 札幌
• [Presentation] Effect of diminished flow in rabbit lumbar arteries on intervertebral disc matrix changes : evaluation with T2 values using 3T-MRI (2011)
  • Author(s): Imanishi T, Akeda K, Sudo A
  • Organizer: The International Society for the Study of the Lumbar Spine
  • Place of Presentation: Gothenburg, Sweden
• [Presentation] Intradiscal injection of autologous serum isolated from platelet-rich-plasma for the treatment of discogenic low back pain : Preliminary prospective clinical trial (2011)
  • Author(s): Akeda K, Imanishi T, Ohishi K, Masuda K, Uchida A, Sakakibara T, Kasai Y
  • Organizer: The International Society for the Study of the Lumbar Spine
  • Place of Presentation: Gothenburg, Sweden
• [Presentation] 多血小板血漿由来血清を用いた椎間板治療の開発-椎間板性疼痛患者に対する臨床試験- (2011)
  • Author(s): 明田浩司、今西隆夫、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第40回日本脊椎脊髄病学会
• [Presentation] 腰動脈狭窄による腰椎椎間板基質代謝への影響 (2011)
  • Author(s): 今西隆夫、明田浩司、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第40回日本脊椎脊髄病学会
• [Presentation] Effect of Platelet-rich Plasma-Serum on the Intervertebral Disc Degeneration : A Preclinical Study (2011)
  • Author(s): Akeda, Koji ; Obata, Shuji ; Masuda, Koichi ; Morimoto, Ryo ; Asanuma, Yumiko ; Kasai, Yuichi ; Sudo, Akihiro
  • Organizer: AAOS 2011 Annual Meeting
  • Place of Presentation: San Diego, Calfornia
• [Presentation] Risk Factors for Lumbar Intervertebral Disc Height Narrowing-Ten Years Retrospective Cohort Study (2011)
  • Author(s): Akeda, Koji ; Yamada, Tomomi ; Inoue, Nozomu ; Kasai, Yuichi ; Sudo, Akihiro
  • Organizer: 57th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach
• [Presentation] Effect of Autologous Platelet-rich Plasma-serum on the Rabbit Anular Needle Puncture Model : Preclinical Study for Intervertebral Disc Degeneration (2011)
  • Author(s): Obata, Shuji ; Akeda, Koji ; Masuda, Koichi ; Morimoto, Ryo ; Imanishi, Takao ; Kasai, Yuichi ; Sudo, Akihiro
  • Organizer: 57th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Long Beach
• [Presentation] 多血小板血漿由来血清を用いた生物学的椎間板治療の開発-椎間板性疼痛患者に対する臨床経験- (2010)
  • Author(s): 明田浩司、今西隆夫、小畑秀司、大石晃嗣、舛田浩一、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第74回東海脊椎脊髄病研究会学術集会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-12-04
• [Presentation] 自己多血小板血漿ゲルの作製と成長因子解析-整形外科手術への応用に向けて- (2010)
  • Author(s): 中村知樹、明田浩司、小畑秀司、大石晃嗣、辻井雅也、若林弘樹、長谷川正裕、松峯昭彦、須藤啓広
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 椎間板バイオマテリアルの開発-糖鎖高分子技術の応用- (2010)
  • Author(s): 明田浩司、小畑秀司、長谷川正裕、宮本啓一、須藤啓広
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 後頭骨頚椎固定術後に発症した上気道閉塞-後頭骨軸椎角をパラメーターとした検討- (2010)
  • Author(s): 明田浩司、浅沼由美子、榊原紀彦、笠井裕一、須藤啓広
  • Organizer: 第115回中部整形外科・災害外科学会・学術集会
  • Place of Presentation: 大阪市
• [Presentation] 腰椎椎間板高狭小化の進行に関わる危険因子解析-地域住民の追跡コホート調査- (2010)
  • Author(s): 明田浩司、笠井裕一、山田知美、井上望、須藤啓広
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知市
• [Presentation] 組織レニン-アンギオテンシン系シグナルはラット椎間板細胞の基質代謝を調節する (2010)
  • Author(s): 森本亮、明田浩司、小畑秀司、笠井裕一、須藤啓広
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知市
• [Presentation] 多血小板血漿由来血清を用いた椎間板変性治療の開発-臨床前研究としての試み- (2010)
  • Author(s): 小畑秀司、明田浩司、森本亮、笠井裕一、浅沼由美子、須藤啓広
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知市
• [Presentation] he Intradiscal Injection of Autologous Platelet-rich Plasma-serum induced the Restoration of Disc Height in the Rabbit Anular Needle Puncture Model (2010)
  • Author(s): Obata Shuji ; Akeda Koji ; Morimoto Ryo ; Asanuma Yumiko ; Masuda Koichi ; Kasai Yuichi ; Uchida Atsumasa ; Sudo Akihiro
  • Organizer: The International Society for the Study of the Lumbar Spine
  • Place of Presentation: uckland, New Zealand
• [Presentation] Risk factors for lumbar intervertebral disc height narrowing. Ten years retrospective cohort study- (2010)
  • Author(s): Koji Akeda, Yuichi Kasai, Nozomu Inoue, Tomomi Yamada, Atsumasa Uchida, Akihiro Sudo
  • Organizer: The International Society for the Study of the Lumbar Spine
  • Place of Presentation: Auckland, New Zealand
• [Presentation] Expression of the Tissue Renin-Angiotensin System in Rat Intervertebral Disc-Implication for Disc Degeneration- (2010)
  • Author(s): Morimoto R ; Akeda K ; Kasai Y ; Iida R ; Nishimura A ; Obata S ; Iino T ; Uchida A ; Sudo A
  • Organizer: The International Society for the Study of the Lumbar Spine
  • Place of Presentation: Auckland, New Zealand
• [Presentation] Function of the Tissue Renin-angiotensin System in Rat Intervertebral Disc Cells (2010)
  • Author(s): Morimoto, Ryo ; Akeda, Koji ; Kasai, Yuichi ; Nishimura, Akinobu ; Obata, Shuji ; Iino, Takahiro ; Uchida, Atsumasa
  • Organizer: 56th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans
• [Presentation] 後頭骨頸椎固定術後に発症した上気道閉塞 後頭骨軸椎角をパラメーターとした検討 (2010)
  • Author(s): 明田浩司、須藤啓広, 他
  • Organizer: 第115回中部日本整形外科災害外科学会
  • Place of Presentation: 大阪市
• [Presentation] 脊椎の1椎間固定が多椎間に与える影響 (2010)
  • Author(s): 榊原紀彦、明田浩司, 他
  • Organizer: 第115回中部日本整形外科災害外科学会
  • Place of Presentation: 大阪市
• [Presentation] 仙椎硬膜外に生じた慢性炎症性肉芽の一例 (2010)
  • Author(s): 淺沼由美子、明田浩司, 他
  • Organizer: 第115回中部日本整形外科災害外科学会
  • Place of Presentation: 大阪市
• [Presentation] 椎間板バイオマテリアルの開発 糖鎖高分子技術の応用 (2010)
  • Author(s): 明田浩司、須藤啓広, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 腰椎椎間板高狭小化の危険因子 10年間の追跡調査 (2010)
  • Author(s): 明田浩司、須藤啓広, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 高齢者転倒に関与する運動機能の検討 第7回旧宮川村検診より (2010)
  • Author(s): 西村明展、明田浩司, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 多血小板血漿由来血清を用いた椎間板再生治療 新たな腰痛治療法の開発 (2010)
  • Author(s): 小畑秀司、明田浩司, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] アクリジンオレンジはマウス骨肉腫の肺転移を抑制する (2010)
  • Author(s): 里中東彦、明田浩司, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 骨格筋の虚血/再灌流障害に対するedaravoneの効果 (2010)
  • Author(s): 堀和一郎、明田浩司, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 脊椎靱帯骨化症におけるVEGF中和抗体の骨化抑制効果 (2010)
  • Author(s): 淺沼由美子、明田浩司, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都市
• [Presentation] 腰椎椎間板高狭小化の進行に関わる危険因子解析 地域住民の追跡コホート調査 (2010)
  • Author(s): 明田浩司、須藤啓広, 他
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知市
• [Presentation] 多血小板血漿由来血清を用いた椎間板変性治療の開発 臨床前研究としての試み (2010)
  • Author(s): 小畑秀司、明田浩司, 他
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知市