L-serine starvation response in osteoclast precursors and its physiological significance in the bone marrow microenvironment
Project/Area Number |
22791382
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Orthopaedic surgery
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
OGAWA Takuya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (30457147)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | アミノ酸 / セリン / 細胞・組織 / 発生・分化 / 発現制御 / シグナル伝達 / 骨・軟骨代謝学 / 破骨細胞 / L-セリン / 分化 / アミノ酸飢餓ストレス |
Research Abstract |
Osteoclasts play central roles in the development and remodeling of bone. In addition to the stimulation by RANKL, serine in the culture medium is necessary for the in vitro differentiation of osteoclasts from their precursors. We conducted a series of experiments to understand the regulatory mechanism of osteoclast differentiation by serine as well as its physiological significance, and found the activation of amino acid starvation response in the precursors by serine deprivation alone, low expression of serine biosynthetic enzymes, and importance of serine supplied by osteoblasts to osteoclasts in the bone marrow microenvironment.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Advantages and Potential of Lipid-Membrane- Incorporating Fullerenes Prepared by the Fullerene-Exchange Method2012
Author(s)
Ikeda, M. Mori, K. Kiguchi, K. Yasuhara, J. Kikuchi, K. Nobusawa, M. Akiyama, M. Hashizume, T. Ogawa, T. Takeya
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Journal Title
Chem. Asian J
Volume: Vol.7, No.3
Issue: 3
Pages: 605-613
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids2012
Author(s)
Yogo, K., Tojima, H., Ohno, J.Y., Ogawa, T., Nakamura, N., ら(計8名)
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Journal Title
Histochem.Cell Biol.
Volume: 137
Issue: 1
Pages: 53-65
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Identification and expression analysis of CX45 and CX60 as major connexins in porcine oocytes2010
Author(s)
Nitta,M., Yogo,K., Ohashi,M., Akiyama,M., Kunitomo,Y., Ogawa,T., Ishida-Kitagawa,N., Miyoshi,J., Sato,E., Takeya,T
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Journal Title
J.Anim.Sci
Volume: Vol.88, No. 10
Issue: 10
Pages: 3269-3279
DOI
Related Report
Peer Reviewed
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[Presentation] 細胞内セリン充足度と脂質代謝関連遺伝子発現の連動2011
Author(s)
月野真理子, 草田航, 佐矢野智子, 濱野桃子, 小川拓哉, 加藤久典, 平林義雄, 古屋茂樹
Organizer
日本アミノ酸学会第5回学術大会
Place of Presentation
名古屋大学シンポシオン(名古屋)(一般演題)(ポスター発表)
Year and Date
2011-11-04
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