Interactions between MSC-containing bone marrow adipose tissue and osteoblasts

• Project/Area Number: 22791387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Saga University
• Principal Investigator: UCHIHASHI Kazuyoshi 佐賀大学, 医学部, 助教 (60452835)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨 / 軟骨代謝学 / 骨髄脂肪 / 骨芽細胞 / 間葉系幹細胞 / 細胞増殖 / 細胞分化

Research Abstract

Bone marrow stromal cells including a small number of lipid-laden preadipocytes and CD44+/ CD105+ mesenchymal stem cell(MSC)-like cells, developed from bone marrow adipose tissue(BMAT) in the organ culture of BMAT. We also examined BMAT-osteoblast interactions and found that osteoblasts inhibited the development of BMSCs and reduced leptin production, while BMAT inhibited the growth and differentiation of osteoblasts.

Research Products

• [Journal Article] A promising culture model for analyzing the interaction between adipose tissue and cardiomyocytes (2011)
  • Author(s): Anan M, Uchihashi K, Aoki S, Matsunobu A, Ootani A, Node K, Toda S.
  • Journal Title: Endocrinology Volume: 152 Pages: 1599-1605
  • Peer Reviewed
• [Journal Article] Interaction between adipose tissue stromal cells and gastric cancer cells in vitro (2011)
  • Author(s): Nomoto-Kojima N, Aoki S, Uchihashi K, Matsunobu A, Koike E, Ootani A, Yonemitsu N, Fujimoto K, Toda S.
  • Journal Title: Cell Tissue Res Volume: 344 Pages: 287-298
  • Peer Reviewed
• [Journal Article] 脂肪組織と脂肪細胞の基礎形態学 (2011)
  • Author(s): 戸田修二、松延亜紀、内橋和芳、山本美保子、薬師寺舞、山崎文朗、小池英介、青木茂久、杉原甫
  • Journal Title: 臨床検査 Volume: 55 Pages: 533-538
  • Peer Reviewed
• [Journal Article] FLUID FLOW STRESS AFFECTS PERITONEAL CELL KINETICS : POSSIBLE PATHOGENESIS OF PERITONEAL FIBROSIS (2011)
  • Author(s): Aoki S, Makino J, Nagashima A, Takezawa T, Nomoto N, Uchihashi K, Matsunobu A, Sanai T, Sugihara H, Toda S.
  • Journal Title: Perit Dial Int Volume: [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture (2011)
  • Author(s): Udo K
  • Journal Title: Cell Tissue Res Volume: 344 Pages: 287-298
  • Peer Reviewed
• [Journal Article] Organotypic culture of human bone marrow adipose tissue (2010)
  • Author(s): Uchihashi K, Aoki S, Shigematsu M, Kamochi N, Sonoda E, Soejima H, Fukudome K, Sugihara H, Hotokebuchi T, Toda S.
  • Journal Title: Pathol Int Volume: 60(4) Pages: 259-67
  • NAID: 10029331372
  • Peer Reviewed
• [Journal Article] メタボリック症候群における脂肪組織の病理形態と病態解析培養モデル (2010)
  • Author(s): 戸田修二、青木茂久、内橋和芳、(他6名、3番目)
  • Journal Title: 病理と臨床 Volume: 28 Pages: 959-965
  • Peer Reviewed
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture (2010)
  • Author(s): Udo K, Aoki S, Uchihashi K, Kawasaki M, Matsunobu A, Tokuda Y, Ootani A, Toda S, Uozumi J.
  • Journal Title: Kidney Int Volume: 78 Pages: 60-68
  • Peer Reviewed
• [Journal Article] The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase (2010)
  • Author(s): Yee CH, Aoki S, Uchihashi K, Matsunobu A, Yamasaki F, Misago N, Piao M, Tetsuji U, Yonemitsu N, Sugihara H, Toda S.
  • Journal Title: Acta Histochem Cytochem Volume: 43 Pages: 1-7
  • NAID: 130000158695
  • Peer Reviewed
• [Journal Article] Culture model for studying thyroid biology and disorders
  • Author(s): Toda S, Aoki S, Uchihashi K, Matsunobu A, Yamamoto M, Ootani A, Yamasaki F, Koike E, Sugihara H.
  • Journal Title: ISRN Endocrinology Volume 2011 Volume: 275782 Pages: 9-9
  • Peer Reviewed
• [Presentation] Adipose Tissue-Derived Stromal Cells Promote the Invasive Growth of Gastric Adenocarcinoma Cells, Which Accelerate the Myofibroblastic Differentiation of the Stromal Cells in Order to Develop a Cancer-Associated Cell Type (2010)
  • Author(s): Nomoto N, Yonemitsu N, Aoki S, Uchihashi K, Matsunobu A, Toda S, Fujimoto K.
  • Organizer: DDW
  • Place of Presentation: Gastroenterology
  • Year and Date: 2010-05-10
• [Presentation] Adipose Tissue-Derived Stromal Cells Promote the Invasive Growth of Gastric A denocarcinoma Cells, Which Accelerate the Myofibroblastic Differentiation of the Stromal Cells in Order to Develop a Cancer-Associated Cell Type (2010)
  • Author(s): Nomoto N
  • Organizer: Digestive disease week (DDW)
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2010-05-10
• [Presentation] A new organotypic culture of adippose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells (2010)
  • Author(s): Uchihashi K, Aoki S, Matsunobu A, Koike E, Yonemitsu N, Funatsumaru S, Sugihara h, Toda S.
  • Organizer: The 14 th International Congress of Endocrinology
  • Place of Presentation: Kyoto, Endocrine Journal
• [Presentation] A new organotypic culture of adippose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells (2010)
  • Author(s): Uchihashi K
  • Organizer: The 14 th International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan
• [Book] 特発性大腿骨頭壊死症 (2010)
  • Author(s): 内橋和芳、馬渡正明
  • Total Pages: 268
  • Publisher: 金芳堂