Functional analysis of novel proteins associated with muscleregeneration.

• Project/Area Number: 22791389
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kumamoto University
• Principal Investigator: NAKAYAMA Yuki 熊本大学, 大学院・自然科学研究科, 准教授 (30332381)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 筋ジストロフィー / 筋再生 / マウス / 骨格筋

Research Abstract

The aim of this study is to investigate the physiological functionof RAMP and Brinp3 whose expression is enhanced in skeletal muscles after injury. Toelucidate the function of RAMP and Brinp3, I tried a yeast two-hybrid screen to identifyits interacting partners. Furthermore, to elucidate the physiological function of RAMPin regenerating muscle, I produced the mice conditionally inactivated RAMP in skeletalmuscle.

Research Products

• [Journal Article] Muscle injury-induced thymosin β4acts as a chemoattractant for myoblasts. (2011)
  • Author(s): §Tokura Y.,§Nakayama Y., Fukuda S.,Nara N., Yamamoto H., Matsuda R., Hara T.
  • Journal Title: JBiochem Volume: 149(1) Pages: 43-48
  • NAID: 10030582799
  • Peer Reviewed
• [Journal Article] Muscle injury-induced thymosin β4 acts as a chemoattractant for myoblasts. (2011)
  • Author(s): Tokura Y、Nakayama Y、
  • Journal Title: J Biochem Volume: 149 Issue: 1 Pages: 43-48
  • DOI: 10.1093/jb/mvq115
  • NAID: 10030582799
  • Peer Reviewed
• [Journal Article] CXCL14deficiency in mice attenuates obesity andinhibits feeding behavior in a novelenvironment. (2010)
  • Author(s): Tanegashima K., Okamoto S., Nakayama Y.,Taya C., Shitara H., Ishii R., Yonekawa H.,Minokoshi Y., Hara T.
  • Journal Title: PLoS ONE Volume: 5(4)
  • Peer Reviewed
• [Journal Article] Antibody-assistedenhancement of biological activities ofCXCL14 in human monocytic leukemia-derivedTHP-1 cells and high fat diet-induced obesemice. (2010)
  • Author(s): Tanegashima K., Suzuki K., Nakayama Y.,Hara T.
  • Journal Title: Exp Cell Res Volume: 316(7) Pages: 1263-1270
  • Peer Reviewed
• [Journal Article] ケモカインCXCL14を介した摂食と糖代謝の調節 (2010)
  • Author(s): 原孝彦、 種子島幸祐、 岡本士毅、 中山由紀、箕越靖彦
  • Journal Title: 自律神経 Volume: 47 Pages: 248-251
  • NAID: 10026905744
• [Journal Article] CXCL14 deficiency in mice attenuates obesity and inhibits feeding behavior in anovel environment. (2010)
  • Author(s): Tanegashima K., et al.
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Antibody-assisted enhancement of biological activities of CXCL14 in human monocytic leukemia-derived THP-1 cells and high fat diet-induced obese mice. (2010)
  • Author(s): Tanegashima K., et al.
  • Journal Title: Experimental Cell Research Volume: 316 Pages: 1263-1270
  • Peer Reviewed
• [Presentation] CXCL14受容体を構成するGタンパク共役型受容体の同定 (2011)
  • Author(s): 鈴木健司
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 筋再生促進候補因子RAMPの結合分子の同定 (2011)
  • Author(s): 中山由紀
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] マウスセルトリ細胞におけるレチノイン酸を介したニューレギュリン発現機構の解析 (2010)
  • Author(s): 植村彩乃、中山由紀、江頭恒、安部眞一
  • Organizer: 第35回比較内分泌学会
  • Place of Presentation: 静岡県コンベンションアーツセンター