The effect of sex hormone on canilaae metabolism
| Project/Area Number |
22791392
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
MINODA Yukihide 大阪市立大学, 大学院・医学研究科・整形外科学, 講師 (90453133)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エストロゲン / 軟骨代謝 / ノックアウトマウス / cre-loxPシステム / ATDC5 |
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| Research Abstract |
The effect of sex hormone on cartilage metabolism is unclear, and little has been reported about the effects of estrogen on the cartilage differentiation. Estrogen is essential for maintaining the balance between osteoblastic and osteoclastic activity during bone remodeling in adults. To investigate the mechanism by which estrogen enhances chondrogenic differentiation, in vitro experiments were carried out using ATDC5 cells(a mouse pre-chondrogenic cell line) and primary cultured chondrogenic cells of mouse rib. Alcian blue staining for cartilage nodules and real-time reverse-transcription polymerase chain reaction for the expression of genes encoding type II collagen. type X collagen and augrecan, were performed in cells treated with estrogen. The expression level of aggrecan and type II collagen in cells, a marker of chondrogenic differentiation, was consistently elevated by low dose of estrogen(10^<-12> M) and was suppressed by high dose(10^<-6> M). ATDC5 and primary cultured chondrogenic cells treated with certain concentration of estrogen have higher expression of Col II and aggrecan.
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Report
(3 results)
Research Products
(44 results)
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[Journal Article] In vivo comparison of wear particles between highly crosslinked polyethylene and conventional polyethylene in the same design of total knee arthroplasties.2009
Author(s)
Iwakiri K, Minoda Y, Kobayashi A, Sugama R, Iwaki H, Inori F, Hashimoto Y, Ohashi H, Ohta Y, Fukunaga K, Takaoka K.
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Journal Title
J Biomed Mater Res B Appl Biomater.
Volume: 91(2)
Pages: 799-804
Related Report
Peer Reviewed
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[Presentation] Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty2012
Author(s)
Minoda Y, Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, lwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
Organizer
Annual meeting of Orthopaedic Reserch Society(ORS)
Place of Presentation
San Francisco, CA, USA.
Year and Date
2012-02-04
Related Report
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[Presentation] The Extension Gap Becomes Tight and Valgus After Implantation of Mobile-bearing Total Knee Prosthesis.2012
Author(s)
Minoda Y Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
Organizer
Annual meeting of Orthopaedic Reserch Society(ORS)
Place of Presentation
San Francisco, CA, USA.
Year and Date
2012-02-04
Related Report
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[Presentation] Operative Technique Affected The Intra-operative Joint Kinematics Of Total Knee Arhtroplasty.2012
Author(s)
Minoda Y. Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
Organizer
Annual meeting of Orthopaedic Reserch Society(ORS)
Place of Presentation
San Francisco, CA, USA.
Year and Date
2012-02-04
Related Report
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[Presentation] Invited lecture ; Future expectation of TKA.2011
Author(s)
Minoda Y, Kadoya Y, Kobayashi A, Iwaki H, Iwakiri K, lida T, Matusi Y, Ikebuchi M, Yoshida T, Nakamura H.
Organizer
24th Annual meeting of the International Society for Technology in Arthroplasty.
Place of Presentation
Brues, Belgium.
Year and Date
2011-09-20
Related Report
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[Presentation] High incidence of anterior femoral cortex notching using navigation system in TKA.2011
Author(s)
Minoda Y, Watanabe K, Iwaki H, Ikebuchi M, Yoshida T, lida T, Ikawa T, Hashimoto Y, Nakamura H.
Organizer
Annual meeting of American Association of Orthopaedic Surgeons.
Place of Presentation
San Diego, CA, USA.
Year and Date
2011-02-15
Related Report
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[Presentation]2011
Author(s)
Minoda Y, Watanabe K, Iwaki H, Lkebuchi M, Yoshida T, Lida T, Ikawa T, Hashimoto Y, Nakamura H.
Organizer
Annual meeting of American Academy of Orthopaedic Surgeons(AAOS)
Place of Presentation
San Diego, CA, USA.
Year and Date
2011-02-07
Related Report
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[Presentation] Comparison of BMD around the Knee between Trabecular Metal and Cemented Tibial Component ; Four-year Follow-Up Study.2011
Author(s)
Minoda Y, Kobayashi A, Ikebuchi M, Iwaki H, Inori F, Hashimoto Y, Yoshida T, lida T, Ikawa T, Yamasaki S, Nakamura H.
Organizer
Annual Meeting of Orthopaedic Research Society.
Place of Presentation
Long Beach, CA, USA.
Year and Date
2011-01-13
Related Report
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[Presentation] Future expectation of TKA2011
Author(s)
Minoda Y
Organizer
24^<th> Annual meeting of the International Society for Technology in Arthroplasty, Invited Lecture
Place of Presentation
Concert Building, Brues, Belgium(招待講演)
Related Report
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