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Elucidation of impaired bone homeostasis mechanism by mutation of Acetaldehyde Dehydrogenase2 gene(ALDH2)

Research Project

Project/Area Number 22791396
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionKeio University

Principal Investigator

HOSHI Hiroko  慶應義塾大学, 医学部, 特任助教 (50399812)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords骨・軟骨代謝学 / アルコール分解酵素 / 骨代謝 / 骨粗鬆症 / 骨芽細胞 / 過酸化脂質 / アセトアルデヒド
Research Abstract

About half of the Japanese have a mutant ALDH2 gene, resulting to sensitivity to alcohol. In statistical analysis report, a mutation of the ALDH2 gene accelerates osteoporosis. However, the mechanism on how the mutated gene ALDH2 affects bone formation is still unknown. In this study, I try to work out the relation between the mutation of the ALDH2 gene and bone homeostasis by utilizing inactive ALDH2(ALDH2-DAL) mice. ALDH2-DAL mice exhibited osteoporosis and showed significantly reduced bone density. In morphometrical analysis, ALDH2-DAL trabecular and cortical bone thickness were thinner, and bone formation and mineralized rates were also decreased compared to that of wild type mice. Osteoblast in mice with mutated ALDH2 gene remarkably decreased compared to wild type osteoblasts by cell staining. The expression of marker genes on osteoblast differentiation of mice with mutated ALDH2-DAL gene was decreased compared to wild type osteoblasts.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (4 results)

  • [Journal Article] Aldehyde-stress resulting from Aldh2 mutation promotes osteoporosis due to impaired osteoblastogenesis2012

    • Author(s)
      Hiroko Hoshi Yoshiaki Toyama, Takeshi Miyamoto, et, al
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] OC-STAMP and DC-STAMP cooperatively modulate cell-cell fusion in osteoclasts and foreign body giant cells2012

    • Author(s)
      Hiroya Miyamoto, Hiroko Hoshi, Takeshi Miyamoto, et, al
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hiroshi Hinou and Shin-Ichro Noshimura. Molecular shuttle between extracellular and cytoplasmic space : Glycosaminoglycan biosynthesis promoted by serglycin precursor glycopeptides in human articular chondrocytes2012

    • Author(s)
      Hiroko Hoshi, Ken Shimawaki, Yasuhiro Takegawa
    • Journal Title

      Biochemica et Biophysica Acta

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aldehyde-stress resulting from Aldh2 mutation promotes osteoporosis due to impaired osteoblastogenesis2012

    • Author(s)
      Hiroko Hoshi., Takeshi Miyamoto., et, al
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: (in press)(印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OC-STAMP and DC-STAMP cooperatively modulate cell-cell fusion in osteoclasts and foreign body giant cells2012

    • Author(s)
      Hiroya Miyamoto., Hiroko Hoshi., Takeshi Miyamoto., et, al
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular shuttle between extracellular and cytoplasmic space : Glycosaminoglycan biosynthesis promoted by serglycin precursor glycopeptides in human articular chondrocytes2012

    • Author(s)
      Hiroko Hoshi., Shin-Ichro Noshimura., et, al
    • Journal Title

      Biochemica et Biophysica Acta

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization2011

    • Author(s)
      Kana Miyamoto., Hiroko Hoshi. and Takeshi Miyamoto., et, al
    • Journal Title

      Journal of Experimental Medicine

      Volume: 208(11) Pages: 2175-2181

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutation of Aldh2 gene suppresses osteoblasts differentiation by accumulation of acetaldehyde and peroxidated lipid-protein2011

    • Author(s)
      Hiroko Hoshi and Takeshi Miyamoto
    • Journal Title

      Osteoporosis International

      Volume: 22 Pages: 605-606

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization2011

    • Author(s)
      Kana Miyamoto., Hiroko Hoshi., Takeshi Miyamoto., et, al
    • Journal Title

      Journal of Experimental Medicine

      Volume: 208 Pages: 2175-2181

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mutation of Aldh2 gene suppresses osteoblasts differentiation by accumulation of acetaldehyde and peroxidated lipid-protein2011

    • Author(s)
      Hiroko Hoshi, Hao Wu, Yoshiaki Toyama and Takeshi Miyamoto
    • Organizer
      2^<nd> Asia-Pacific Osteoporosis and Bone Meeting
    • Place of Presentation
      Gold Coast, Australia
    • Year and Date
      2011-09-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Mutation of Aldh2 gene suppresses osteoblasts differentiation by accumulation of acetaldehyde and peroxidated lipid-protein2011

    • Author(s)
      Hiroko Hoshi, Takeshi Miyamoto
    • Organizer
      2^<nd> Asia-Pacific Osteoporosis and Bone Meeting
    • Place of Presentation
      Gold Coast convention center (Gold Coast, Austraria)
    • Year and Date
      2011-09-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aldh2遺伝子変異はアセトアルデヒド及び酸化脂質の蓄積により骨芽細胞の分化抑制をきたす2011

    • Author(s)
      星淡子、宮本健史, 他
    • Organizer
      第29回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2011-07-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Aldh2遺伝子変異はアセトアルデヒド及び酸化脂質の蓄積により骨芽細胞の分化抑制をきたす2011

    • Author(s)
      星淡子、宮本健史
    • Organizer
      第29回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2011-07-30
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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