Spinal cord repair by re-myelination enhancement. Elucidation of the functional recovery mechanism after cell transplantation for spinal cord injury-

• Project/Area Number: 22791397
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: YASUDA Akimasa 慶應義塾大学, 医学部, 助教(非常勤) (40445226)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脊髄損傷 / 神経幹細胞 / 細胞移植 / 再髄鞘化 / shiverer mutant マウス

Research Abstract

We have reported that transplantation of neural stem/progenitor cells(NS/PCs) for spinal cord injury promoted functional recovery, while the precise mechanism of functional recovery still remains unclear. In the present study, we utilized NS/PCs derived from genetically myelin deficient shiverer mutant mice. We compared shiverer mouse derived NS/PCs with wild type mouse derived NS/PCs. We have shown that graft-derived re-myelination is significant in the functional recovery after neural stem/progenitor cell transplantation after spinal cord injury.

Research Products

• [Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior (2012)
  • Author(s): Hara-Miyauchi, C. C., Tsuji, O. O., Hanyu, A. A., Okada, S. S., Yasuda, A. A., Fukano, T. T., Akazawa, C. C.
  • Journal Title: Biochemical and biophysical research communications Volume: 419(2) Pages: 188-193
  • Peer Reviewed
• [Journal Article] Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord (2011)
  • Author(s): Yasuda, A., O. Tsuji, S. Shibata, S. Nori, M. Takano, Y. Kobayashi, Y. Takahashi, K. Fujiyoshi, C. M. Hara, A. Miyawaki, H. J. Okano, Y. Toyama, M. Nakamura, and H. Okano
  • Journal Title: Stem Cells Volume: 29(12) Pages: 1983-94
  • Peer Reviewed
• [Journal Article] Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition (2011)
  • Author(s): Renault-Mihara, F., H. Katoh, T. Ikegami, A. Iwanami, M. Mukaino, A. Yasuda, S. Nori, Y. Mabuchi, H. Tada, S. Shibata, K. Saito, M. Matsushita, K. Kaibuchi, S. Okada, Y. Toyama, M. Nakamura, and H. Okano
  • Journal Title: EMBO Mol Med Volume: 3(11) Pages: 682-96
  • Peer Reviewed
• [Journal Article] Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice-Their In Vitro Characterization and Therapeutic Potential (2011)
  • Author(s): Takagi, T., K. Ishii, S. Shibata, A. Yasuda, M. Sato, N. Nagoshi, H. Saito, H. J. Okano, Y. Toyama, H. Okano, and M. Nakamura
  • Journal Title: PloS one Volume: 6(6)
  • URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021497
  • Peer Reviewed
• [Journal Article] Grafted human-induced pluripotent stem-cell-derived neurospheres promote motor functional recovery after spinal cord injury in mice (2011)
  • Author(s): Nori, S., Y. Okada, A. Yasuda, O. Tsuji, Y. Takahashi, Y. Kobayashi, K. Fujiyoshi, M. Koike, Y. Uchiyama, E. Ikeda, Y. Toyama, S. Yamanaka, M. Nakamura, and H. Okano
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Pages: 1-10
  • Peer Reviewed
• [Journal Article] Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord. (2011)
  • Author(s): Yasuda A, Tsuji O, Shibata S, Nori S, Takano M, Kobayashi Y, Takahashi Y, Fujiyoshi K, Hara CM, Miyawaki A, Okano HJ, Toyama Y, Nakamura M, Okano H
  • Journal Title: Stem Cells Volume: 29(12) Issue: 12 Pages: 1983-94
  • DOI: 10.1002/stem.767
  • Peer Reviewed
• [Journal Article] 【脊髄損傷治療の現状とこれから】人工多能性幹(iPS)細胞を用いた脊髄損傷治療 (2011)
  • Author(s): 安田明正, ら
  • Journal Title: 脳21 Volume: 14 Pages: 126-132
• [Presentation] Significance of re-myelination by Neural stem/protenitor cells grafted into the injured spinal cord (2011)
  • Author(s): Yasuda A, et al
  • Organizer: 41st Annual meeting Neuroscience 2011
  • Place of Presentation: ワシントンDC、USA
  • Year and Date: 2011-11-13
• [Presentation] 脊髄損傷に対する神経幹細胞移植後の機能回復における再髄鞘化の重要性 (2011)
  • Author(s): 安田明正, ら
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア高崎(群馬)
  • Year and Date: 2011-10-20
• [Presentation] Significance of re-myelination after NS/PC transplantation to SCI (2011)
  • Author(s): Yasuda A, et al
  • Organizer: ISSCR 9th annual meeting
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2011-06-16
• [Presentation] 脊髄損傷に対する神経幹細胞移植後の機能回復における再髄鞘化の重要性 (2011)
  • Author(s): 安田明正, ら
  • Organizer: 第40回脊椎脊髄病学会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2011-04-21
• [Presentation] Significance of re-myelination by Neural stem/protenitor cells grafted into the injured spinal cord (2011)
  • Author(s): Yasuda, A., Tsuji, O., Shibata, S., Fujiyoshi, K., Takahashi, Y., Nori, S., Kobayashi, Y., Toyama, Y., Nakamura, M., Okano, H.
  • Organizer: 41st Annual meeting Neuroscience 2011
  • Place of Presentation: Washington Walter E Convention Center, Washington D. C., USA
• [Presentation] 脊髄損傷に対する神経幹細胞移植後の機能回復における再髄鞘化の重要性 (2011)
  • Author(s): 安田明正、辻収彦、芝田晋介、藤吉兼浩、高橋勇一朗、海苔聡、小林喜臣、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 高崎
• [Presentation] Significance of re-myelination after NS/PC transplantation to SCI (2011)
  • Author(s): Yasuda, A., Tsuji, O., Shibata, S., Fujiyoshi, K., Takahashi, Y., Nori, S., Kobayashi, Y., Toyama, Y., Nakamura, M., Okano, H.
  • Organizer: ISSCR 9th annual meeting
  • Place of Presentation: Metro Convention Center, Toronto, Canada
• [Presentation] 脊髄損傷に対する神経幹細胞移植後の機能回復における再髄鞘化の重要性先天性脱髄マウス由来神経幹細胞を用いた解析 (2011)
  • Author(s): 安田明正、辻収彦、芝田晋介、藤吉兼浩、高橋勇一朗、海苔聡、小林喜臣、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第40回脊椎脊髄病学会
  • Place of Presentation: 東京
• [Presentation] 損傷脊髄への神経幹細胞移植療法における再髄鞘化の重要性-先天性脱随マウス由来神経幹細胞を用いた検討- (2011)
  • Author(s): 安田明正、辻収彦、海苔聡、高橋勇一朗、藤吉兼浩、高野盛登、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] Significance of re-myelination after NS/PC transplantation to SCI. (2010)
  • Author(s): Yasuda A., et al
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] 損傷脊髄への神経幹細胞移植療法における再髄鞘化の重要性-先天性脱随マウス由来神経幹細胞を用いた検討- (2010)
  • Author(s): 安田明正, ら
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-14
• [Presentation] Significance of re-myelination in the functional recovery after NS/PC transplantation to SCI (2010)
  • Author(s): 安田明正辻収彦海苔聡高橋勇一朗藤吉兼浩戸山芳明中村雅也岡野栄之
  • Organizer: Neuroscience 2010
  • Place of Presentation: サンディエゴ、USA
• [Presentation] 損傷脊髄の再生において再髄鞘化が及ぼす影響の検討-神経幹細胞移植療法における行動機能回復メカニズムの解析- (2010)
  • Author(s): 安田明正、辻収彦、芝田晋介、海苔聡、高橋勇一朗、藤吉兼浩、戸山芳昭、中村雅也、岡野栄之
  • Organizer: Neuro2010(第33回日本神経科学大会)
  • Place of Presentation: 神戸
• [Presentation] 損傷脊髄の再生において再髄鞘化が及ぼす影響の検討-神経幹細胞移植療法における行動機能回復メカニズムの解析- (2010)
  • Author(s): 安田明正、辻収彦、海苔聡、高橋勇一朗、藤吉兼浩、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知