Schwann Cell Plasticity after Spinal Cord Injury Shown by Neural Crest Lineage Cells

• Project/Area Number: 22791398
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: NAGOSHI Narihito 慶應義塾大学, 医学部, 助教 (10383837)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脊髄損傷 / シュワン細胞 / 再生医療 / 脱分化 / 再分化 / 髄鞘化 / 神経堤細胞

Research Abstract

After spinal cord injury(SCI), various cell types are recruited to the lesion site, including Schwann cells, which originate in the neural crest. Here, we investigated the differentiation states, migration patterns, and roles of neural crest derivatives following SCI, using a transgenic mouse line carrying neural crest-specific reporters, P0-Cre/Floxed-EGFP. This study draws on the advantages offered by transgenic mouse lines bearing a genetic cell-lineage marker and extends previous work by describing the origins and behavior of the neural crest-derived cells that contribute to endogenous repair after SCI. This process is a novel repair mechanism for the lesioned mammalian spinal cord.

Research Products

• [Journal Article] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2011)
  • Author(s): Nagoshi N, Shibata S, Hamanoue M, Mabuchi Y, Matsuzaki Y, Toyama Y, Nakamura M, Okano H
  • Journal Title: Glia Volume: 59 Pages: 771-84
  • Peer Reviewed
• [Journal Article] Dysfunction of fibroblasts of extrarenal origin underlies renal fibrosis and renal anemia in mice (2011)
  • Author(s): Asada N, Takase M, Nakamura J, Oguchi A, Asada M, Suzuki N, Yamamura KI, Nagoshi N, Shibata S, Rao TN, Fehling HJ, Fukatsu A, Minegishi N, Kita T, Kimura T, Okano H, Yamamoto M, Yanagita M
  • Journal Title: J Clin Invest Volume: 121 Pages: 3981-90
  • NAID: 120003338854
  • Peer Reviewed
• [Journal Article] Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero (2011)
  • Author(s): Kubota Y, Takubo K, Hirashima M, Nagoshi N, Kishi K, Okuno Y, Nakamura-Ishizu A, Sano K, Murakami M, Ema M, Omatsu Y, Takahashi S, Nagasawa T, Shibuya M, Okano H, Suda T
  • Journal Title: J Exp Med Volume: 208 Pages: 949-60
  • Peer Reviewed
• [Journal Article] Schwann-Spheres Derived from Injured Peripheral Nerves in Adult Mice-Their In Vitro Characterization and Therapeutic Potential (2011)
  • Author(s): Takagi T, Ishii K, Shibata S, Yasuda A'^2, Sato M, Nagoshi N, Saito H, Okano HJ, Toyama Y, Okano H, Nakamura M
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] The dual origin of the peripheral olfactory system : placode and neural crest (2011)
  • Author(s): Katoh H, Shibata S, Fukuda K, Sato M, Satoh E, Nagoshi N, Minematsu T, Matsuzaki Y, Akazawa C, Toyama Y, Nakamura M, Okano H
  • Journal Title: Mol Brain Volume: 4 Pages: 34-34
  • Peer Reviewed
• [Journal Article] Schwann cell plasticity after spinal cord injury shown by neural crest lineage cells (2011)
  • Author(s): Nagoshi N, et al
  • Journal Title: Glia Volume: 59 Pages: 771-84
  • Peer Reviewed
• [Journal Article] Sox10-Venus mice : a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes (2010)
  • Author(s): Shibata S, Yasuda A, Renault-Mihara F, Suyama S, Katoh H, Inoue T, Inoue YU, Nagoshi N, Sato M, Nakamura M, Akazawa C, Okano H
  • Journal Title: Mol Brain Volume: 3 Pages: 31-31
  • Peer Reviewed
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture (2010)
  • Author(s): Aihara Y, Hayashi Y, Hirata M, Ariki N, Shibata S, Nagoshi N, Nakanishi M, Ohnuma K, Warashina M, Michiue T, Uchiyama H, Okano H, Asashima M, Furue MK
  • Journal Title: Int J Dev Biol Volume: 54 Pages: 1287-94
  • Peer Reviewed
• [Journal Article] Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury (2010)
  • Author(s): Tsuji O, Miura K, Okada Y, Fujiyoshi K, Mukaino M, Nagoshi N, Kitamura K, Kumagai G, Nishino M, Tomisato S, Higashi H, Nagai T, Katoh H, Kohda K, Matsuzaki Y, Yuzaki M, Ikeda E, Toyama Y, Nakamura M, Yamanaka S, Okano H
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 12704-9
  • Peer Reviewed
• [Journal Article] Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury (2010)
  • Author(s): Tsuji O, et al.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 12704-9
  • Peer Reviewed
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture (2010)
  • Author(s): Aihara Y, et al.
  • Journal Title: The International Journal of Developmental Biology Volume: 54 Pages: 1287-94
  • Peer Reviewed
• [Journal Article] Sox10-Venus mice : a new tool for real-time labeling of neural crest line age cells and oligodendrocytes (2010)
  • Author(s): Shibata S, et al.
  • Journal Title: Molecular Brain Volume: 3 Pages: 31-31
  • Peer Reviewed
• [Presentation] 神経堤幹細胞:損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2011)
  • Author(s): 名越慈人、芝田晋介、松崎有未、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第46回脊椎脊髄障害医学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-18
• [Presentation] 損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2011)
  • Author(s): 名越慈人, 他
  • Organizer: 第46回脊椎脊髄障害医学会
  • Place of Presentation: 関西空港会議場(大阪)
  • Year and Date: 2011-11-18
• [Presentation] 神経堤幹細胞:その多能性と再生医療における有用性 (2011)
  • Author(s): 名越慈人、芝田晋介、松崎有未、中村雅也、戸山芳昭、岡野栄之
  • Organizer: 第53回歯科基礎医学会学術集会サテライトシンポジウム
  • Place of Presentation: 岐阜
  • Year and Date: 2011-09-30
• [Presentation] 神経堤幹細胞:その多能性と再生医療における有用性 (2011)
  • Author(s): 名越慈人, 他
  • Organizer: 第53回歯科基礎医学会学術集会サテライトシンポジウム
  • Place of Presentation: 長良川国際会議場(岐阜)(招待講演)
  • Year and Date: 2011-09-30
• [Presentation] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2011)
  • Author(s): Nagoshi N, Shibata S, Matsuzaki Y, Okano H, Toyama Y, Nakamura M
  • Organizer: The 2nd Cervical Spine Research Society Asia Pacific Section
  • Place of Presentation: Korea
  • Year and Date: 2011-04-29
• [Presentation] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2011)
  • Author(s): Nagoshi N, et al
  • Organizer: The 2nd Cervical Spine Research Society Asia Pacific Section
  • Place of Presentation: 釜山・韓国
  • Year and Date: 2011-04-29
• [Presentation] 神経堤幹細胞:損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2010)
  • Author(s): 名越慈人、芝田晋介、松崎有未、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第64回国立病院総合医学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-11-26
• [Presentation] 損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2010)
  • Author(s): 名越慈人, 他
  • Organizer: 第64回国立病院総合医学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-11-26
• [Presentation] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2010)
  • Author(s): Nagoshi N, Shibata S, Matsuzaki Y, Okano H, Toyama Y, Nakamura M
  • Organizer: Society for Neuroscience 2010
  • Place of Presentation: USA
  • Year and Date: 2010-11-17
• [Presentation] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2010)
  • Author(s): Nagoshi N, et al.
  • Organizer: Society for Neuroscience 2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-17
• [Presentation] 神経堤幹細胞の発生と多能生～損傷脊髄の再生を目指して～ (2010)
  • Author(s): 名越慈人, 他
  • Organizer: 第83回日本整形外科学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-27
• [Presentation] 神経堤幹細胞:損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2010)
  • Author(s): 名越慈人、芝田晋介、松崎有未、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知
  • Year and Date: 2010-04-23
• [Presentation] 損傷脊髄における自己修復機構-内在性シュワン細胞の寄与- (2010)
  • Author(s): 名越慈人, 他
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知
  • Year and Date: 2010-04-23