| Project/Area Number |
22791404
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
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| Keywords | 脊椎脊髄病学 / ES/iPS細胞 / コモンマーモセット / 脊髄損傷 / 再生医療 |
|---|
| Research Abstract |
We established in vitro culture method to efficiently derive multipotent neural stem/progenitor cells(NS/PCs) from non-human primate, common marmoset embryonic stem cells(ESCs). These marmoset ESC-derived neurospheres could be passaged repeatedly and sequentially gave rise to neurons and glial cells, including both astrocytes and oligodendrocytes. Importantly, marmoset ESC-derived NS/PCs could generate functional neurons, and showed closer expression profiles to fetal brain-derived neurosphres by repeated passages. Thus, this culture system is applicable to derive donor cells for allograft transplantation into marmoset models.
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