Efficient derivation of neural stem/progenitor cells from common marmoset embryonic stem cells for preclinical study of spinal cord injury.

• Project/Area Number: 22791404
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: SHIMADA Hiroko 慶應義塾大学, 医学部, 研究員 (60528644)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 脊椎脊髄病学 / ES/iPS細胞 / コモンマーモセット / 脊髄損傷 / 再生医療

Research Abstract

We established in vitro culture method to efficiently derive multipotent neural stem/progenitor cells(NS/PCs) from non-human primate, common marmoset embryonic stem cells(ESCs). These marmoset ESC-derived neurospheres could be passaged repeatedly and sequentially gave rise to neurons and glial cells, including both astrocytes and oligodendrocytes. Importantly, marmoset ESC-derived NS/PCs could generate functional neurons, and showed closer expression profiles to fetal brain-derived neurosphres by repeated passages. Thus, this culture system is applicable to derive donor cells for allograft transplantation into marmoset models.

Research Products

• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells. (2012)
  • Author(s): Hiroko Shimada, Yohei Okada, Hayao Ebise, Shin-ichi Ota, Ikuo Tomioka, Erika Sasaki, Masaya Nakamura, Hideyuki Okano
  • Organizer: Frontiers in Biomedical Researches on Marmosets as a Primate Model Japan Society for Marmoset Research Tokyo Medical and Dental University
  • Place of Presentation: Tokyo, Japan
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2012)
  • Author(s): 嶋田弘子
  • Organizer: Frontiers in Biomedical Researches on Marmosets as a Primate Model, Japan Society for Marmoset Research
  • Place of Presentation: Tokyo Medical and Dental University, Tokyo, Japan
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): 嶋田弘子
  • Organizer: Neuroscience 2011, SfN's 41st annual meeting
  • Place of Presentation: The Walter E.Washington Convention Center, Washington, DC, U.S.A
  • Year and Date: 2011-11-15
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): 嶋田弘子
  • Organizer: Neuroscience 2011、第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川県、日本
  • Year and Date: 2011-09-17
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): 嶋田弘子
  • Organizer: ISSCR 9th Annual Meeting
  • Place of Presentation: Metro Toronto Convention Center, Toronto, Ontario, Canada
  • Year and Date: 2011-06-16
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): Hiroko Shimada, Yohei Okada, Hayao Ebise, Shin-ichi Ota, Ikuo Tomioka, Erika Sasaki, Masaya Nakamura, Hideyuki Okano
  • Organizer: Neuroscience 2011, SfN's 41st annual meeting
  • Place of Presentation: The Walter E. Washington Convention Center, Washington, DC, U. S. A.
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): Hiroko Shimada, Yohei Okada, Ikuo Tomioka, Erika Sasaki, Masaya Nakamura, Hideyuki Okano
  • Organizer: Neuroscience2011
  • Place of Presentation: Yokohama
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells (2011)
  • Author(s): Hiroko Shimada, Yohei Okada, Ikuo Tomioka, Erika Sasaki, Masaya Nakamura, Hideyuki Okano
  • Organizer: ISSCR 9th Annual Meeting
  • Place of Presentation: Toronto, Canada