| Project/Area Number |
22791409
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University (2011)
Japanese Foundation For Cancer Research (2010) |
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Principal Investigator |
HIKITA Atsuhiko 愛媛大学, 大学院・医学系研究科, 准教授 (60443397)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | RANKL / RANK / 破骨細胞 / インビボ蛍光イメージング / BiFC / 蛍光イメージング / 蛍光in vivoイメージング / RANKL-RANK |
|---|
| Research Abstract |
By utilizing a fluorescence detection system by binding of complementary parts of a fluorescent protein(BiFC), the genes needed for the detection of the interaction of RANK and RANKL, proteins necessary for the formation of osteoclasts whoch have a central role in bone resorption, were constructed. In a preliminary experiment in which these two genes were introduced into cells, fluorescence was detected under the condition when RANK and RANKL could bind together.
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