Comprehensive measurement of proteinases and proteinase inhibitors within osteoarthritic knee cartilage and synovial fluid collected from osteoarthritic knee joints

Research Project

Project/Area Number	22791411
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Orthopaedic surgery
Research Institution	Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
Principal Investigator	IWASAWA Mituyasu 独立行政法人国立病院機構(相模原病院臨床研究センター), 統括診療部・整形外科, 医師 (60574093)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	変形性関節症 / タンパク分解酵素 / 関節軟骨 / ウロキナーゼ / プラスミン
Research Abstract	In order to determine the amounts of proteinases within OA cartilage, proteins were extracted from cartilage matrix, and concentrations of various proteinases were determined by ELISA and Luminex, together with those of endogenous proteinase inhibitors. Synovial fluid was collected from OA knees, and the amounts of proteinases and endogenous inhibitors were determined as well. The results of our analysis revealed that OA cartilage contains various kinds of proteinases besides MMP-13 and ADAMTS-4 and 5, which have been supposed to be primary proteinases in OA. Again, our study has revealed that the synovial fluid from OA joints contains considerable amounts of MMP-1, 2 and 3 together with TIMP-1 and 2.