Cellular and molecular biological analysis of the impact of drugs used during periorerative period on cellular oxygen metabolism

• Project/Area Number: 22791428
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kyoto University
• Principal Investigator: TAKABUCHI Satoshi 京都大学, 医学研究科, 研究員 (90402841)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 薬理学 / 麻酔薬 / 低酸素応答 / 細胞生物学 / 分子生物学 / 麻酔科学

Research Abstract

We demonstrated that Glucose-stimulated insulin secretion(GSIS) elicited by 400 mg/dL glucose was inhibited by treatment with isoflurane and sevoflurane. Taken together experimental evidence that glibenclamide-stimulated insulin secretion was not affected by either isoflurane or sevoflurane and that treatment with isoflurane or sevoflurane significantly suppress glucose-induced intracellular ATP elevation, it is suggested that isoflurane and sevoflurane affect mitochondria ETC and suppresses GSIS. Our finding may delineate the molecular mechanism behind the glucose tolerance established by volatile anesthetics.

Research Products

• [Journal Article] Hydrogen sulfide inhibits hypoxia- but not anoxia-induced hypoxia-inducible factor 1 activation in a von hippel-lindau- and mitochondria-dependent manner. (2011)
  • Author(s): 甲斐慎一、田中具治、大条紘樹、原田浩、岸本俊、鈴木堅悟、高淵聡史、竹永敬三、福田和彦、広田喜一
  • Journal Title: Antioxid Redox Signal Volume: 16 Issue: 3 Pages: 203-216
  • DOI: 10.1089/ars.2011.3882
  • Peer Reviewed
• [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice under non-hypoxic conditions in a μ-opioid receptor-dependent manner. (2011)
  • Author(s): 大条紘樹、甲斐慎一、田中具治、若松拓彦、岸本俊、鈴木堅悟、原田浩、高淵聡史、足立健彦、福田和彦、広田喜一
  • Journal Title: Eur J Pharmacol Volume: 667 Issue: 1-3 Pages: 144-152
  • DOI: 10.1016/j.ejphar.2011.06.014
  • Peer Reviewed
• [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages (2010)
  • Author(s): 田中具治、高淵聡史、西憲一郎、小田聖子、若松拓彦、大条紘樹、福田和彦、広田喜一
  • Journal Title: J Anesth Volume: 24 Issue: 1 Pages: 54-60
  • DOI: 10.1007/s00540-009-0829-1
  • NAID: 10027265604
  • Peer Reviewed
• [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages. (2010)
  • Author(s): Tanaka, Tomohiro, 他
  • Journal Title: Journal of Anesthesia Volume: 24 Pages: 54-60
  • Peer Reviewed
• [Presentation] Differential role of prostaglandin E-type receptors in activation of hypoxia-inducible factor1 by prostaglandin E1 in vascular-derived cells under non-hypoxic conditions (2011)
  • Author(s): Kengo Suzuki, Satoshi Takabuchi, Hiroki Daijo, Shinichi Kai, Tomonori Matsuyama, Shin Kurosawa, Kazuhiko Fukuda, Kiichi Hirota
  • Organizer: 2011Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-16
• [Presentation] Hydrogen sulfide inhibits hypoxia-induced HIF-1 activation in a mitochondria-dependent manner (2011)
  • Author(s): Shinichi Kai, Tomoharu Tanaka, Hiroki Daijo, Shun Kishimoto, Kengo Suzuki, Satoshi Takabuchi, Takuhiko Wakamatsu, Kazuhiko Fukuda, Kiichi Hirota
  • Organizer: 2011 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-16