Involvement of microenvironment constituted by intra-spinal oxygen metablism in establishment of neuropathic pain

• Project/Area Number: 22791429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kyoto University
• Principal Investigator: WAKAMATSU Kakuhiko 京都大学, 医学研究科, 研究員 (20551451)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 慢性疼痛 / 麻酔科学 / 低酸素応答 / マイクログリア / 神経因性疼痛

Research Abstract

In response to activation of microglia, HIF-1 activation and expression of genes, which play essential roles in neuropathic pain, are observed under 20% conditions. In particular, LPS and ATP induced P2X4 expression, which is most strongly related to neuropathic pain development than P2X7, under 20% O2 conditions and much more induce under 1% O2 conditions. These findings suggest that redox environment caused by micro oxygen environment in spinal cord may affect activation of microglia and gene expression involved in neuropathic pain.

Research Products

• [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronalSH-SY5Y cells and mice under non-hypoxicconditions in a mu-opioidreceptor-dependent manner (2011)
  • Author(s): Daijo, H., Kai, S., Tanaka, T., Wakamatsu, T., Kishimoto, S., Suzuki, K., Harada, H., Takabuchi, S., Adachi, T., Fukuda, K., Hirota, K
  • Journal Title: Europeanjournal of pharmacology Volume: 667 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice under non-hypoxic conditions in a μ-opioid receptor-dependent manner. (2011)
  • Author(s): 大条紘樹、甲斐慎一、田中具治、若松拓彦、岸本俊、鈴木堅悟、原田浩、高淵聡史、足立健彦、福田和彦、広田喜一
  • Journal Title: Eur J Pharmacol Volume: 667 Issue: 1-3 Pages: 144-152
  • DOI: 10.1016/j.ejphar.2011.06.014
  • Peer Reviewed
• [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages (2010)
  • Author(s): 田中具治、高淵聡史、西憲一郎、小田聖子、若松拓彦、大条紘樹、福田和彦、広田喜一
  • Journal Title: J Anesth Volume: 24 Issue: 1 Pages: 54-60
  • DOI: 10.1007/s00540-009-0829-1
  • NAID: 10027265604
  • Peer Reviewed
• [Journal Article] Persisting mild hypothermia suppresses hypoxia-inducible factor 1{alpha} protein synthesis and HIF-1-mediated gene expression. (2010)
  • Author(s): Tanaka, Tomohiro, 他
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. Volume: 293
  • Peer Reviewed
• [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages. (2010)
  • Author(s): Tanaka, Tomohiro, 他
  • Journal Title: Journal of Anesthesia Volume: 24 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Persistingmild hypothermia suppresseshypoxia-inducible factor-1alpha proteinsynthesis and hypoxia-induciblefactor-1-mediated gene expression (2009)
  • Author(s): Tanaka, T., Wakamatsu, T., Daijo, H., Oda, S., Kai, S., Adachi, T., Kizaka-Kondoh, S., Fukuda, K., and Hirota, K
  • Journal Title: American journal of physiology Regulatory, integrative and comparative physiology Volume: 298 Pages: 661-671
  • Peer Reviewed
• [Presentation] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice under non-hypoxic conditions in aオ-opioidreceptor-dependent manner (2011)
  • Author(s): H. Daijo, K. Hirota, S. Kai1, T. Matsuyama, K. Suzuki T. Tanaka, T. Wakamatsu, K. Fukuda
  • Organizer: 2011 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-15