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Gene therapy for neuropathic pain applying SNP pace maker ion-channel (HCN channel) transduction to the spinal cord and dorsal root ganglion

Research Project

Project/Area Number 22791433
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Anesthesiology/Resuscitation studies
Research InstitutionOkayama University

Principal Investigator

KAKU Ryuji  岡山大学, 大学院・医歯薬学総合研究科, 助教 (50444659)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords遺伝子治療 / 神経因性疼痛 / 慢性痛 / ペースメーカーチャンネル / 神経障害性痛 / グリコシル化 / 慢性疼痛
Research Abstract

HCN ion channels also know as a pace-maker channel play an important role on regulating spontaneous firing of central nerve systems at various neuropathic pain state. In this study, we investigated about the mechanisms of the HCN ion channel expression change to the nerve system. And we also investigated that the possibility of dominant-negative HCN ion channel as the source for the gene therapy to chronic neuropathic pain. We found that increase of cyclic AMP concentration decreased the glycosylated HCN ion channels on the plasma membrane. Non-glycosylated (SNP) HCN channels could reach to the plasma membrane of xenopus oocyte, but it did not pass the current. These findings suggested that the modulation of HCN channel expression on the spinal cord might be useful as the new treatment of chronic neuropathic pain.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2013 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (7 results)

  • [Journal Article] Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia2013

    • Author(s)
      Mizobuchi S 、 Kanzaki H 、 Omiya H 、Matsuoka Y、Obata N、Kaku R、Nakajima H、Ouchida M、Morita K
    • Journal Title

      J Pain Res

      Volume: 6巻 Pages: 87-94

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antinociceptive effects of intrathecal landiolol injection in a rat formalin pain model2012

    • Author(s)
      Mizobuchi S、Matsuoka Y、Obata N、Kaku R、Itano Y、Tomotsuka N、Taniguchi A、Nishie H、Kanzaki H、Ouchida M、Morita K
    • Journal Title

      Acta Med Okayama

      Volume: 66巻 Pages: 285-289

    • NAID

      120004247100

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extracellular regulated kinase 5 mediated renal protection against ischemia-reperfusion injury2012

    • Author(s)
      Kawakami T、Park SW、Kaku R、Yang J
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 418巻 Pages: 603-608

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression changes of the neuregulin 1 isoforms in neuropathic pain model rats2012

    • Author(s)
      Kanzaki H、Mizobuchi S、Obata N、Itano Y、Kaku R、Tomotsuka N、Nakajima H、Ouchida M、Nakatsuka H、Maeshima K、Morita K
    • Journal Title

      Neurosci Lett

      Volume: 508巻 Pages: 78-83

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antinociceptive effects of intrathecal landiolol injection in a rat formalin pain model2012

    • Author(s)
      Mizobuchi S
    • Journal Title

      Acta Med Okayama

      Volume: 66 Pages: 285-289

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Extracellular-regulated-kinase 5-mediated renal protection against ischemia-reperfusion injury2012

    • Author(s)
      Kawakami T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 418 Issue: 4 Pages: 603-608

    • DOI

      10.1016/j.bbrc.2012.01.043

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression changes of the neuregulin 1 isoforms in neuropathic pain model rats2012

    • Author(s)
      Kanzaki H
    • Journal Title

      Neurosci Lett

      Volume: 508 Issue: 2 Pages: 78-83

    • DOI

      10.1016/j.neulet.2011.12.023

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats2011

    • Author(s)
      Obata N、Mizobuchi S、Itano Y、Matsuoka Y、Kaku R、Tomotsuka N、Morita K、Kanzaki H、Ouchida M、Yokoyama M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 408巻 Pages: 139-44

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Decoy strategy targeting the Brain-derived neurotrophic factor Exon I suppress pain behavior in rat inflammatory pain model2012

    • Author(s)
      Taniguchi A、Kaku R、Tomotsuka N、Omiya H 、 Ishii N 、 Obata N 、 Matsuoka Y 、Mizobuchi S、Morita K
    • Organizer
      American Society of Anesthesiologists Annual meeting
    • Place of Presentation
      ワシントンDC、USA
    • Year and Date
      2012-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Change of BDNF Expression in the Dorsal Root Ganglion of the Bone Cancer Pain Model2012

    • Author(s)
      Tomotsuka N、Kaku R、Taniguchi A、Obata N、Matsuoka Y、Mizobuchi S、Sato T、Ichikawa H、Morita K
    • Organizer
      American Society of Anesthesiologists Annual meeting
    • Place of Presentation
      ワシントンDC、USA
    • Year and Date
      2012-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Decoy Strategy Targeting the Brain-derived Neurotrophic Factor Exon I Suppress Pain Behavior in Rat Inflammatory Pain Model2012

    • Author(s)
      Taniguchi A
    • Organizer
      American Society of Anesthesiologists
    • Place of Presentation
      Washington, D.C.
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of the Cyclic AMP in Glycosylation and Plasma Membrane Targeting of the HCN Ion Channels2010

    • Author(s)
      Kaku R、Obata N、Kanzaki H、Yang J、Morita K
    • Organizer
      American Society of Anesthesiologists Annual meeting
    • Place of Presentation
      サンディエゴ、USA
    • Year and Date
      2010-10-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of the Cyclic AMP in Glycosylation and Plasma Membrane Targeting of the HCN Ion Channels2010

    • Author(s)
      賀来隆治
    • Organizer
      2010米国麻酔学会学術総会
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2010-10-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] HCNチャンネルの転写後調節による 細胞膜表面への発現調節に関する検討2010

    • Author(s)
      賀来隆治、小幡典彦、友塚直人、板野義太郎、溝渕知司、森田潔
    • Organizer
      日本疼痛学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-07-02
    • Related Report
      2012 Final Research Report
  • [Presentation] HCNチャンネルの転写後調節による細胞膜表面への発現調節に関する検討2010

    • Author(s)
      賀来隆治
    • Organizer
      第32回日本疼痛学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-07-02
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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