Analysis of analgesic action of remifentanil through dimerized mu-delta opioid receptors

• Project/Area Number: 22791441
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagasaki University
• Principal Investigator: MURATA Hiroaki 長崎大学, 大学病院, 助教 (90437856)
• Research Collaborator: UEZONO Yasuhito 独立行政法人国立がん研究センター, 研究所, 分野長 (20213340) ; SUDO Yuka 独立行政法人国立がん研究センター, 研究所, 研究支援者 (70646695)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: オピオイド受容体 / 二量体 / レミフェンタニル / 急性耐性

Research Abstract

Baby hamster kidney cells transfected with Venus-fused mu opioid receptors and Cerulean-fused delta opioid receptors were treated with remifentanil. To investigate the remifentanil-induced internalization and resensitization of the heterodimers, cells were fixed and examined by confocal microscopy after treatment of remifentanil. Fluorescence resonance energy transfer analysis was performed to confirm the heterodimerization of mu opioid receptors and delta opioid receptors. Remifentanil caused internalization of mu-delta heterodimers in a dose-dependent manner. Compared to our previous study using baby hamster kidney cells transfected with mu opioid receptors alone, baby hamster kidney cells transfected with mu opioid receptors and delta opioid receptors were more efficiently returned to plasma membrane after the remifentanil-induced internalization.

Research Products

• [Journal Article] GABA(B) receptors do not internalize after baclofen treatment, possibly due to a lack of ss-arrestin association: study with a real-time visualizing assay (2012)
  • Author(s): 須藤結香、北條美能留、安藤優子、高田正史、村田寛明、倉田眞治、西田教行、上園保仁
  • Journal Title: Synapse Volume: 66 Pages: 759-769
  • Peer Reviewed
• [Journal Article] GABA_B receptors do not internalize after baclofen treatment possibly due to lack of theβ-arrestin association ; a real-time visualization study (2012)
  • Author(s): Sudo, Y., Hojo, M., Ando, Y., Takada, M., Murata, H., Kurata, S., Kanaide, M., Nishida, N., Uezono, Y.
  • Journal Title: Synapse, Epub ahead of print Volume: 66 (9) Issue: 9 Pages: 759-769
  • DOI: 10.1002/syn.21565
  • Peer Reviewed
• [Journal Article] GABAB receptors do not internalize after baclofen treatment, possibly due to a lack of ss-arrestin association : study with a real-time visualizing assay
  • Author(s): 須藤結香、村田寛明、上園保仁, 他
  • Journal Title: Synapse Volume: (in press)
  • Peer Reviewed
• [Presentation] 細胞膜移行性シグナルペプチドを付加したHaloTag-GPCR の発現様式ならびにその機能的アッセイ (2012)
  • Author(s): 須藤結香、宮野加奈子、村田寛明、北條美能留、長瀬隆弘、西田教行、上園保仁
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-15
• [Presentation] 細胞移行性シグナルペプチドを付加したHaloTag-GPCRの発現様式並びにその機能的アッセイ (2011)
  • Author(s): 須藤結香、村田寛明、上園保仁, 他
  • Organizer: 第64回薬理学会西南部会
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-19