| Project/Area Number |
22791446
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Miyazaki |
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Principal Investigator |
TAMURA Ryuji 宮崎大学, 医学部, 助教 (40549060)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 麻酔学 / 局所麻酔薬 / 腫瘍特異性 / アポトーシス / ミトコンドリア / リドカイン / HL60 / フリーラジカル |
|---|
| Research Abstract |
The aim of this study was to clarify the local anesthetics that induce apoptosis or necrosis and their induction-related factors.
Methods : Apoptosis and necrosis were analyzed by double-staining assay with propidium iodide(PI) and annexin-V and were measured by flow cytometry(FACS). DNA fragmentation was used for the analysis of apoptosis.
Conclusion : The results indicate that local anesthetics with high lipophilicity are highly toxic and induce mainly necrosis, while local anesthetics with low pKa induce more apoptosis.
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