Functional analysis of water channel in ischemic brain injury after cardiac arrest

• Project/Area Number: 22791448
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagoya City University
• Principal Investigator: SOU Mine 名古屋市立大学, 大学院・医学研究科, 助教 (60381886)
• Co-Investigator(Renkei-kenkyūsha): ASAI Kiyofumi 名古屋市立大学, 大学院・医学研究科, 教授 (70212462) ; SOBUE Kazuya 名古屋市立大学, 大学院・医学研究科, 教授 (90264738) ; ARIMA Hajime 名古屋市立大学, 大学院・医学研究科, 講師 (20254295) ; HIRATE Hiroyuki 名古屋市立大学, 大学院・医学研究科, 助教 (20363939)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 蘇生学 / 蘇生 / 水チャネル / 脳浮腫

Research Abstract

Astrocytic swelling is one of the mechanisms of brain edema. We found that AQP4 enhanced expression which rose by ischemia-reoxygenation relates to astrocytic swelling. Participation of AQP4 was suggested to the cerebral edema by brain ischemia in an animal model. When astrocyte was medicated with the inhibitory drug(SN-50) of NF-kB, or the inhibitory drug(SB203580)of p38 MAPK and ischemia-reoxygenation was caused, the tendency for the rapid revelation rise of AQP4 to be controlled was acquired. Although the further examination was required, a possibility that regulation of cerebral edema could be performed by this research was suggested.

Research Products

• [Journal Article] Diclofenac enhances proinflammatory cytokine-induced aquaporin-4 expression in cultured astrocyte (2013)
  • Author(s): Asai H, Kakita H, Aoyama M, Nagaya Y, Saitoh S, Asai K.
  • Journal Title: Cell Mol Neurobiol. Volume: 33 Issue: 3 Pages: 393-400
  • DOI: 10.1007/s10571-013-9905-z
  • Peer Reviewed
• [Journal Article] Subventricular zone-derived oligodendrogenesis in injured neonatal white matter in mice enhanced by a nonerythropoietic erythropoietin derivative. (2012)
  • Author(s): Kako E, Kaneko N, Aoyama M, Hida H, Takebayashi H, Ikenaka K, Asai K, TogariH, Sobue K, Sawamoto K.
  • Journal Title: Stem Cells Volume: 30 Issue: 10 Pages: 2234-47
  • DOI: 10.1002/stem.1202
  • Peer Reviewed
• [Journal Article] 神経保護Q&A-神経救急・集中治療的アプローチの実際‐神経救急・集中治療の重要性と必要な基礎知識および脳保護・脳蘇生の展望 (2011)
  • Author(s): 祖父江和哉、有馬一、藤田義人
  • Journal Title: 脳障害の病態と血液脳関門. 救急・集中治療 Volume: 23(7-8) Pages: 1013-8
• [Journal Article] A New Enteral Diet, MHN-02, Which Contains Abundant Antioxidants and Whey Peptide, Protects Carbon Tetrachloride-Induced Hepatitis (2011)
  • Author(s): Takayanagi T, Sasaki H, Kawashima A, Mizuochi Y, Hirate H, Sugiura T, Azami T, Asai K, Sobue K
  • Journal Title: JPEN J Parenter Enteral Nutr Volume: 35(4) Issue: 4 Pages: 516-22
  • DOI: 10.1177/0148607110381599
• [Journal Article] 水チャネル<アクアポリン>の中枢神経における多様な機能と関連疾患. (2010)
  • Author(s): 祖父江和哉、高柳猛彦、平手博之、杉浦健之、藤田義人、浅井清文
  • Journal Title: 日本歯科麻酔学会雑誌 Volume: 38(2) Pages: 145-50
  • NAID: 10026404857
• [Presentation] Evaluation of anesthetic factors on characteristics of emergence from anesthesia: Why do patients bite the orotracheal tube? (2011)
  • Author(s): So M, Arima H, Suzuki E, Kako E, Sobue K
  • Organizer: Annual Meeting of International Anesthesia Research Society(IARS) Vancouver
  • Place of Presentation: Canada
• [Presentation] アシアロエリスロポエチンの脳低酸素虚血に対する治療効果 (2011)
  • Author(s): 加古英介、金子奈穂子、青山峰芳、戸苅創、祖父江和哉、澤本和延
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸