Development of a diagnostic method for malignant hyperthermia indexed by cell death provoked by stimulation of ryanodine 1 receptors in monocytes
| Project/Area Number |
22791453
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 麻酔学 / 悪性高熱症 / リアノジン受容体 / ブピバカイン / カフェイン / ミトコンドリア / 細胞障害 / ネクローシス / 麻酔 / 単球・マクロファージ / 細胞死 / アポトーシス |
|---|
| Research Abstract |
To develop a new diagnostic method for malignant hyperthermia, utility of necrotic cytotoxicity assay provoked by agonists for ryanodine-1 receptors(RyR1) in monocytes was evaluated. Two RyR1 agonists, bupivacaine as well as caffeine, elevated intracellular Ca^<2+> concentration. However, bupivacaine but not caffeine provoked cell death. Differential effects of these RyR1 agonists on mitochondrial membrane potential mimicked those on necrotic cell death. These indicated that intracellular Ca^<2+> mobilization was not the direct cause of cell death provoked by RyR1 agonists. Further evaluation concerning the relationship between mitochondrial damage and necrosis provoked by RyR1 agonists should be required.
|
Report
(3 results)
Research Products
(9 results)
