| Project/Area Number |
22791453
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
|
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 麻酔学 / 悪性高熱症 / リアノジン受容体 / ブピバカイン / カフェイン / ミトコンドリア / 細胞障害 / ネクローシス / 麻酔 / 単球・マクロファージ / 細胞死 / アポトーシス |
|---|
| Research Abstract |
To develop a new diagnostic method for malignant hyperthermia, utility of necrotic cytotoxicity assay provoked by agonists for ryanodine-1 receptors(RyR1) in monocytes was evaluated. Two RyR1 agonists, bupivacaine as well as caffeine, elevated intracellular Ca^<2+> concentration. However, bupivacaine but not caffeine provoked cell death. Differential effects of these RyR1 agonists on mitochondrial membrane potential mimicked those on necrotic cell death. These indicated that intracellular Ca^<2+> mobilization was not the direct cause of cell death provoked by RyR1 agonists. Further evaluation concerning the relationship between mitochondrial damage and necrosis provoked by RyR1 agonists should be required.
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