Development of novel intravesical agents for the treatment of refractory superficial bladder cancer

• Project/Area Number: 22791473
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: WATANABE Masami 岡山大学, 岡山大学病院, 准教授 (70444677)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 膀胱癌 / 遺伝子治療 / 抗癌免疫 / 分化 / 癌

Research Abstract

Bladder cancer is one of the most common urogenital malignancies. The intravesical instillation of anti-cancer agent is an attractive strategy to treat a superficial lesion or floating/disseminated cancer cells after transurethral operation. An adenovirus carrying REIC/Dkk-3, a tumor suppressor gene,(Ad-REIC) exhibits cancer-specific apoptotic effects in various types of cancer cells. In this study, we demonstrated that the intravesical instillation of Ad-REIC could be an attractive therapeutic method for the treatment of human bladder cancer.

Research Products

• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1. (2012)
  • Author(s): Jin Y, Murata H, Sakaguchi M, Kataoka K, Watanabe M, Nasu Y, Kumon H
  • Journal Title: Huh NH. Oncol Rep. Volume: 27(3) Pages: 695-9
  • DOI: 10.3892/or.2011.1543
  • Peer Reviewed
• [Journal Article] Preclinical safety and efficacy of in situ REIC/Dkk-3 gene therapy for prostate cancer (2012)
  • Author(s): Kawauchi K, Watanabe M, Kaku H, Huang P, Sasaki K, Sakaguchi M, Ochiai K, Huh NH, Nasu Y, Kumon H
  • Journal Title: Acta Med Okayama Volume: 66(1) Pages: 7-16
  • NAID: 120003850603
  • Peer Reviewed
• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1 (2012)
  • Author(s): Jin Y, Murata H, et al
  • Journal Title: Oncol Rep Volume: 27 Pages: 695-9
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H
  • Journal Title: Biochem Biophys Res Commun Volume: 412(2) Pages: 391-5
  • Peer Reviewed
• [Journal Article] Single Nucleotide Polymorphism WRN Leu1074Phe Is Associated with Prostate Cancer Susceptibility in Chinese Subjects (2011)
  • Author(s): Lei Wang, Haruki Kaku, Peng Huang, Kexin Xu, Kai Yang, Jiheng Zhang, Ming Li, Liping Xie, Xiaofeng Wang, Akiko Sakai, Masami Watanabe, Yasutomo Nasu, Kenji Shimizu, Hiromi Kumon, Yanqun Na
  • Journal Title: Acta Med Okayama Volume: (In press)
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging. (2011)
  • Author(s): Watanabe M, Ueki H, Ochiai K, Huang P, Kobayashi Y, Nasu Y, Sasaki K, Kaku H, Kashiwakura Y, Kumon H
  • Journal Title: Oncol Rep
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence (2011)
  • Author(s): Huang P, Chen J, Wang L, Na Y, Kaku H, Ueki H, Sasaki K, Yamaguchi K, Zhang K, Saika T, Nasu Y, Watanabe M, Kumon H
  • Journal Title: Med Oncol
  • Peer Reviewed
• [Journal Article] Tumor growth and metastasis suppression by Glipr1 gene-modified macrophages in a metastatic prostate cancer model. (2011)
  • Author(s): Tabata K, Kurosaka S, Watanabe M, Edamura K, Satoh T, Yang G, Abdelfattah E, Wang J, Goltsov A, Floryk D, Thompson TC
  • Journal Title: Gene Ther
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai K, Watanabe M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 391-5
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. (2010)
  • Author(s): Zhang K, et al.
  • Journal Title: Int J Oncol. Volume: 37 Pages: 1495-1501
  • Peer Reviewed
• [Journal Article] Potent antitumor effects of combined therapy with a telomerase-specific, replication-competent adenovirus (OBP-301) and IL-2 in a mouse model of renal cell carcinoma. (2010)
  • Author(s): Huang P, et al.
  • Journal Title: Cancer Gene Ther. Volume: 17 Pages: 484-491
  • Peer Reviewed
• [Book] REIC/Dkk-3 gene therapy (2011)
  • Author(s): Watanabe M, Kaku H, Huan g P, Kumon H, Nasu Y
  • Publisher: Nihon Rinsho
• [Book] Suicide gene and immuno gene therapy for prostate cancer (2011)
  • Author(s): Nasu Y, Sasaki K, Kumon H, Kaku H, Watanabe M
  • Publisher: Nihon Rinsho
• [Patent(Industrial Property Rights)] 遺伝子発現を上昇させるシステム及び該システムを保持したベクター (2011)
  • Inventor(s): 公文裕巳, 他
  • Industrial Property Rights Holder: 岡山大学, 他
  • Filing Date: 2011-01-14
  • Overseas