A search for the interaction molecules with REIC/Dkk-3 in prostate gland.
| Project/Area Number |
22791476
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Nippon Veterinary and Life Science University (2011)
The University of Tokushima (2010) |
|---|
Principal Investigator |
OCHIAI Kazuhiko 日本獣医生命科学大学, 獣医学部, 助教 (30550488)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 癌 / 遺伝子 / REIC/Dkk-3 / REIC / Dkk-3 / 前立腺がん / 相互作用 / Tctex-1 / Dkk3 |
|---|
| Research Abstract |
REIC/Dkk-3 acts as a tumor suppressor in multiple cancer cell lines by inducing apoptosis through endoplasmic reticulum(ER) stress signaling. However, the intracellular interaction partners of REIC/Dkk-3 have not been fully elucidated. By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3.The binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-E-X-G-R-R-X-H-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain(DIC). The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively.
|
Report
(3 results)
Research Products
(18 results)
-
[Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer2012
Author(s)
Kawauchi, K., Watanabe, M., Kaku, H., Huang, P., Sasaki, K., Sakaguchi, M., Ochiai, K., Huh, NH., Nasu, Y., and Kumon, H.
-
Journal Title
Acta Med. Okayama
Volume: 66(1)
Pages: 7-16
URL
Related Report
Peer Reviewed
-
-
-
[Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-12011
Author(s)
Ochiai, K., Watanabe, M., Ueki, H., Huang, P., Fujii, Y., Nasu, Y., Sakaguchi, M., Huh, NH., Kashiwakura, Y., Kaku, H., and Kumon, H.
-
Journal Title
Biochem. Biophy. Res. Com
Volume: 412(2)
Issue: 2
Pages: 391-395
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
