A search for the interaction molecules with REIC/Dkk-3 in prostate gland.

• Project/Area Number: 22791476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Nippon Veterinary and Life Science University (2011); The University of Tokushima (2010)
• Principal Investigator: OCHIAI Kazuhiko 日本獣医生命科学大学, 獣医学部, 助教 (30550488)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 癌 / 遺伝子 / REIC/Dkk-3 / REIC / Dkk-3 / 前立腺がん / 相互作用 / Tctex-1 / Dkk3

Research Abstract

REIC/Dkk-3 acts as a tumor suppressor in multiple cancer cell lines by inducing apoptosis through endoplasmic reticulum(ER) stress signaling. However, the intracellular interaction partners of REIC/Dkk-3 have not been fully elucidated. By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3.The binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-E-X-G-R-R-X-H-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain(DIC). The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively.

Research Products

• [Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer (2012)
  • Author(s): Kawauchi, K., Watanabe, M., Kaku, H., Huang, P., Sasaki, K., Sakaguchi, M., Ochiai, K., Huh, NH., Nasu, Y., and Kumon, H.
  • Journal Title: Acta Med. Okayama Volume: 66(1) Pages: 7-16
  • URL: http://www.lib.okayama-u.ac.jp/www/acta/pdf/66_1_7.pdf
  • Peer Reviewed
• [Journal Article] Reduction of protein phosphatase 2A C・enhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix (2012)
  • Author(s): Okamura, et al
  • Journal Title: Bone Volume: 49 Issue: 3 Pages: 368-375
  • DOI: 10.1016/j.bone.2011.06.004
  • Peer Reviewed
• [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats (2011)
  • Author(s): Ochiai, K., et al.
  • Journal Title: The Veterinary Journal Volume: 190 Issue: 2 Pages: 293-295
  • DOI: 10.1016/j.tvjl.2010.11.001
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai, K., Watanabe, M., Ueki, H., Huang, P., Fujii, Y., Nasu, Y., Sakaguchi, M., Huh, NH., Kashiwakura, Y., Kaku, H., and Kumon, H.
  • Journal Title: Biochem. Biophy. Res. Com Volume: 412(2) Issue: 2 Pages: 391-395
  • DOI: 10.1016/j.bbrc.2011.07.109
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging. (2011)
  • Author(s): Watanabe M, Ueki H, Ochiai K, Huang P, Kobayashi Y, Nasu Y, Sasaki K, Kaku H, Kashiwakura Y, Kumon H
  • Journal Title: Oncol Rep. Volume: 26(4) Pages: 769-75
  • DOI: 10.3892/or.2011.1371
  • Peer Reviewed
• [Journal Article] Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 (2011)
  • Author(s): Ochiai, K., et al.
  • Journal Title: FEBS letters Volume: 585 Issue: 12 Pages: 1771-1777
  • DOI: 10.1016/j.febslet.2011.05.027
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development (2010)
  • Author(s): Zhang, K.,Watanabe, M, (他17人)
  • Journal Title: Int. J. Oncol Volume: 37 Issue: 6 Pages: 1495-1501
  • DOI: 10.3892/ijo_00000802
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. (2010)
  • Author(s): Zhang, K., et al.
  • Journal Title: International Journal of Oncology Volume: 37 Pages: 1495-1501
  • Peer Reviewed
• [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats.
  • Author(s): Ochiai, K., et al.
  • Journal Title: The Veterinary Journal Volume: (In press)
  • Peer Reviewed
• [Presentation] イヌおよびヒトBRCA2のBRC repeat 3領域におけるミスセンス変異の比較 (2011)
  • Author(s): 吉川泰永、森松正美、落合和彦、折野宏一、相田成一、垰田高広、近澤征史朗、岩井聡美、渡辺清隆
  • Organizer: 第34回日本分子生物学会年会
  • Year and Date: 2011-12-15
• [Presentation] イヌおよびヒトBRCA2のBRC repeat 3領域におけるミスセンス変異の比較 (2011)
  • Author(s): 吉川泰永, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] イヌ乳腺腫瘍関連遺伝子産物BRCA2のBRC repeat 4のアミノ酸がDNA相同組換え修復に必須なRAD51ホモ会合体形成に与える影響 (2011)
  • Author(s): 落合和彦、吉川泰永、近江俊徳、森松正美
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Presentation] イヌにおけるヒトABO組織血液型遺伝子に相同なfull length cDNAの同定と特徴 (2011)
  • Author(s): 近江俊徳、鄭英和、市東正幸、宇田川智野、河上剛、落合和彦、杉山将、土田修一
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Presentation] 癌抑制遺伝子BRCA2のPCR法による変異解析法の確立 (2011)
  • Author(s): 吉川泰永、森松正美、鈴木優、西村百合香、落合和彦、折野宏一、垰田高広、近澤征史朗、岩井聡美、島村麻子、新井敏郎、渡辺清隆
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Presentation] イヌ乳腺腫瘍関連遺伝子産物BRCA2のBRC repeat 4のアミノ酸がDNA相同組換え修復に必須なRAD51ホモ会合体形成に与える影響 (2011)
  • Author(s): 落合和彦, 他
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] 遺伝性乳がん原因遺伝子BRCA2の動物種間における機能比較 (2011)
  • Author(s): 落合和彦
  • Organizer: 日本病態生理学会第3回サテライトセミナー
  • Place of Presentation: 日本大学医学部
  • Year and Date: 2011-08-19
• [Patent(Industrial Property Rights)] REIC/Dkk-3タンパク質の部分領域ポリペプチド (2011)
  • Inventor(s): 公文裕巳、渡部昌実、二見淳一郎、藤井康之、植木英雄、落合和彦
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Filing Date: 2011-07-01
  • Overseas
• [Patent(Industrial Property Rights)] REIC/Dkk-3タンパク質の部分領域ポリペプチド (2011)
  • Inventor(s): 落合和彦, 他
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Filing Date: 2011-07-01
  • Overseas